标注蛋白说明

1、CLProtein NameAccession No.Protein MWProtein PIProteinScoreProteinScoreC. I. %蛋白名称-3.305130 chloroplast sedoheptulose-1,7-bisphosphatase Morus alba var. multicaulisgi|11817592942824.96.06356100景天庚酮糖-1,7-二磷酸酶桑树-2.893090 PREDICTED: 30S ribosomal protein 2, chloroplastic-like Fragaria vesca subsp. vesc

2、agi|47014157826303.89.157799.826核糖体蛋白30S野草莓-2.893090 PREDICTED: ribulose-phosphate 3-epimerase,chloroplastic-like Fragaria vesca subsp. vescagi|470126730300558.92165100核酮糖-磷酸-3-差向异构酶-2.797190 Probable protein disulfide-isomerase A6 OS=Medicago sativa PE=1 SV=1 40808.85.447999.287二硫键异构酶-2.797190 Prot

3、ease Do-like 1, chloroplastic OS=Arabidopsis thaliana GN=DEGP1 PE=1 SV=2 46816.56.007698.789Protease Do-like 1-2.781850 glyeraldehyde 3-phosphate dehydrogenase Rosa multifloragi|35325970336539.17.68234100甘油醛-3-磷酸脱氢酶野草莓-2.722660 chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase activase Mor

4、us alba var. multicaulisgi|11985547527345.54.76675100核酮糖-1,5-二磷酸羧化酶/加氧酶-2.344520 PREDICTED: cysteine synthase-like Fragaria vesca subsp. vescagi|470132276345415.498799.983半胱氨酸合成酶白梨-2.344520 PREDICTED: succinyl-CoA ligase ADP-forming subunit alpha-1, mitochondrial-like Fragaria vesca subsp. vescagi|4

5、7012698935348.59.06204100琥珀酰基辅酶A白梨-2.313030 chloroplast Mn-superoxide dismutase 1B-d Prunuspersicagi|38338610726083.68.579699.998SOD酶桃-2.236500 PREDICTED: 26S protease regulatory subunit 6A homolog A-like Fragaria vesca subsp. vescagi|47013304947833.65.02627100蛋白酶调控亚基26S野草莓-2.158890 PREDICTED: serin

6、e/arginine-rich splicing factor 33-like Fragaria vesca subsp. vescagi|47011772230062.211.497099.166精氨酸-丝氨酸富集剪接因子33野草莓-1.992800 PREDICTED: 2-dehydro-3-deoxyphosphooctonate aldolase 1-like Fragaria vesca subsp. vescagi|47014166732073.86.61210100醛缩酶型 2脱氢-3-脱氧 野草莓-1.84426methionine sulfoxide reductase M

7、orus alba var. multicaulisgi|37286410021874.76.3362100蛋氨酸硫氧化物还原酶桑树-1.77162PREDICTED: soluble inorganic pyrophosphatase-like Fragaria vesca subsp. vescagi|47014177824206.45.67149100可溶性无机焦磷酸酶野草莓-1.77162PREDICTED: gamma carbonic anhydrase 1,mitochondrial-like Fragaria vesca subsp. vescagi|4701112532963

8、3.46.19299.995碳酸酐酶1野草莓-1.67692PREDICTED: 20 kDa chaperonin, chloroplastic-like Fragaria vesca subsp. vescagi|47010243826123.19.03152100伴侣蛋白20 kDa野草莓-1.59956PREDICTED: regulator of ribonuclease-like protein 3-like Fragaria vesca subsp. vescagi|47011744526953.78.599499.997核糖核酸调节酶-3野草莓-1.559810 hypothe

9、tical protein PRUPE_ppa006841mg Prunus persicagi|46241995043337.95.68460100S-腺苷甲硫氨酸合成酶桃-1.52408glutathione peroxidase 6, partial Fragaria x ananassagi|47649085724730.79.25153100谷胱甘肽过氧化酶草莓-1.51375actin, partial Malus domesticagi|35532994440369.45.67651100肌动蛋白亚基苹果1.62532porin Prunus armeniacagi|503127

10、929764.47.057799.822孔蛋白大扁杏1.95422Deoxyuridine 5'-triphosphate nucleotidohydrolase OS=Arabidopsis thaliana GN=DUT PE=1 SV=117603.25.34106100脱氧尿苷5-三磷酸，核苷酸水解酶拟南芥1.967800 Nucleoside diphosphate kinase OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) GN=ndk1 PE=3 SV=11

11、7035.77.8183100核苷二磷酸激酶A粗糙脉孢菌1.967800 Adenylosuccinate synthetase OS=Aquifex aeolicus (strain VF5) GN=purA PE=3 SV=149051.56.027095腺苷酸琥珀酸合成酶桃2.086590 PREDICTED: ATP synthase subunit beta,mitochondrial-like Fragaria vesca subsp. vescagi|47012718359266.96.068699.981ATP合成酶亚基野草莓2.086590 PREDICTED: non-sp

12、ecific lipid-transfer protein-like Fragaria vesca subsp. vescagi|47010965713608.29.576697.704脂转移蛋白非特异性野草莓2.310140 Bifunctional DNA-directed RNA polymerase subunit beta-beta' OS=Wolbachia pipientis wMel GN=rpoBC PE=3 SV=1319977.06.027899.123RNA聚合酶野草莓2.310140 Xylose isomerase OS=Rhizobium loti (st

13、rain MAFF303099) GN=xylA PE=3 SV=1 49383.86.116892.184木糖异构酶野草莓2.496840 PREDICTED: DEAD-box ATP-dependent RNA helicase 37-like Fragaria vesca subsp. vescagi|47010486170313.86.435678.573DEAD-box RNA解旋酶水葛2.496840 maturase K Pouzolzia mixtagi|37210009829662.59.525362.763成熟酶K2.496840 PREDICTED: mitochond

14、rial dicarboxylate/tricarboxylate transporter DTC-like Fragaria vesca subsp. vesca gi|47011774332144.69.455784.831二/三羧酸转运蛋白酶野草莓2.964970 Translation initiation factor IF-2 OS=Leuconostoc citreum (strain KM20) GN=infB PE=3 SV=191089.29.188399.71 翻译起始因子桃2.964970 Mitochondrial inner membrane protease AT

15、P23 OS=Ustilago maydis (strain 521 / FGSC 9021) GN=ATP23 PE=3 SV=1 25174.27.17094.071线粒体内膜蛋白酶ATP23玉米黑粉菌2.964970 Kinesin-like protein KIF20B OS=Mus musculus GN=Kif20b PE=1 SV=3204985.75.617296.666纺锤体驱动蛋白3.128760 mitochondrial NAD-dependent malic acid dehydrogenase Pyrus pyrifoliagi|31544025635604.98.

16、988099.919苹果酸脱氢酶沙梨3.221110 PREDICTED: ATPase 10, plasma membrane-type-likeFragaria vesca subsp. vescagi|470135969105284.65.346396.01质膜ATP酶野草莓3.286490 PREDICTED: protein SEC13 homolog Fragaria vesca subsp. vesca persicagi|47013304133072.15.27183100 SEC13桃从 2 个桑椹蛋白质的2DE 凝胶中，切取差异表达的45个蛋白斑点，经胶内酶切、质谱分析及数

17、据库检索，结果成功鉴定了其中的38个蛋白斑点。在对照与感病桑椹中，38个差异表达蛋白斑点上调表达17个，下调表达21个。下调蛋白为，光合作用：景天庚酮糖-1,7-二磷酸酶，Protease Do-like 1，核酮糖-1,5-二磷酸羧化酶/加氧酶，碳酸酐酶1，表达翻译：核糖体蛋白30S，二硫键异构酶，蛋白酶调控亚基26S，伴侣蛋白20 kDa，代谢过程：核酮糖-磷酸-3-差向异构酶，甘油醛-3-磷酸脱氢酶，半胱氨酸合成酶，琥珀酰基辅酶A，可溶性无机焦磷酸酶，抗氧化：SOD酶，蛋氨酸硫氧化物还原酶，谷胱甘肽过氧化酶，转录调控：精氨酸-丝氨酸富集剪接因子33，核糖核酸调节酶-3，水解酶：醛缩酶，逆

18、境相关：S-腺苷甲硫氨酸合成酶（参与乙烯形成），结构蛋白：肌动蛋白亚基，能量提供减少。光合作用减弱，表达翻译减弱，抗氧化能力降低，结构蛋白减少，果实成熟相关基因表达降低。上调表达蛋白主要为，孔蛋白：结构蛋白1（细胞膜通透性），SEC13，质膜ATP酶，核苷酸水解酶，脱氧尿苷5-三磷酸，糖代谢：苹果酸脱氢酶，木糖异构酶，细胞分裂：纺锤体驱动蛋白。代谢过程：线粒体内膜蛋白酶ATP23，二/三羧酸转运蛋白酶，ATP合成酶亚基，核苷二磷酸激酶A，翻译：翻译起始因子，转录调控：成熟酶K ，DEAD-box RNA解旋酶，RNA聚合酶，抗性：脂转移蛋白非特异性，生物合成：腺苷酸琥珀酸合成酶细胞膜通透性增加

19、，糖被降解利用，核苷酸水解，转录调控增加，细胞分裂加速，部分代谢过程增强， 说明在病原菌侵染过程中，桑葚光合作用，细胞骨架，部分的代谢过程，及抗氧化能力降低。而细胞的通透性大大提高，转录活性增强，细胞分裂加快，部分代谢途径加快（糖代谢），抗逆信号增强。 同时还有部分蛋白没有确定其性能。Protein NameAccession No.Protein MWProtein PIProteinScoreProteinScoreC. I. %蛋白名称PREDICTED: enolase-like Fragaria vesca subsp. vesca vescagi|47013438848251.65

20、.76365100烯醇化酶（野草莓）ATP synthase CF1 beta subunit Morus indicagi|11480427253815.15.46985100ATP合成酶亚基NAD-dependent malate dehydrogenase Prunus armeniacagi|30770711034811.85.92292100NAD依赖苹果酸脱氢酶（大扁杏）cytosolic malate dehydrogenase Malus domesticagi|7821649335970.56.01171100胞内苹果酸脱氢酶（苹果）PREDICTED: pentatrico

21、peptide repeat-containing protein At4g20740-like Fragaria vesca subsp. vescagi|470139321125589.38.756294.618PPR蛋白（野草莓）PREDICTED: oxygen-evolving enhancer protein 1, chloroplastic-like Fragaria vesca subsp. vescagi|470129332353546.1413100放氧增强子蛋白(野草莓)OEE1ribulose bisphosphate carboxylase Malus sp.gi|1

22、958120718.49.048199.932核酮糖-1,5-二磷酸羧化酶（苹果）chloroplast photosynthetic water oxidation complex 33kDa subunit precursor Morus nigragi|15214364028477.55.48788100叶绿体光合作用水氧化33KDa亚基前体（黑桑茎皮）光合作用水分解反应蛋白前体PREDICTED: thioredoxin reductase 2-like Fragaria vesca subsp. vescagi|47010252235769.15.84192100硫氧还蛋白还原酶（野

23、草莓）PREDICTED: coproporphyrinogen-III oxidase, chloroplastic-like Fragaria vesca subsp. vescagi|47013753743834.66.15287100粪卟啉原氧化酶 III (野草莓)PREDICTED: 60S acidic ribosomal protein P2B-like Fragaria vesca subsp. vescagi|470134336114484.5621510060s酸性核糖体蛋白 P2B(野草莓)PREDICTED: 1-acyl-sn-glycerol-3-phosphat

24、e acyltransferase 2-like Fragaria vesca subsp. vescagi|47014014844110.18.026698脂酰甘油3磷酸酰基转移酶（野草莓）LPAATenoyl-ACP reductase Malus domesticagi|8256944641610.38.728199.931烯酰酸性磷酸酶还原酶（苹果）cytochrome cgi|22947112149.69.886193.961细胞色素 cPREDICTED: cytochrome c oxidase subunit 6b-1-like Fragaria vesca subsp. ve

25、scagi|47012742820119.44.33137100细胞色素c氧化酶亚基(野草莓)PREDICTED: quinone oxidoreductase-like protein At1g23740, chloroplastic-like Fragaria vesca subsp.vescagi|47012323642124.48.73181100醌氧化还原酶蛋白(野草莓)PREDICTED: 2-Cys peroxiredoxin BAS1-like,chloroplastic-like Fragaria vesca subsp. vescagi|47013540228882.96.

26、9199100过氧化物氧化还原酶BAS1(野草莓)putative CC-NBS-LRR resistance protein Malus domesticagi|86438846108432.88.136192.905 CC-NBS-LRR（苹果）Lea protein precursor Glycine maxgi|35172792349483.77.089399.95lea蛋白前体（大豆）PREDICTED: cysteine synthase-like Fragaria vesca subsp. vescagi|47012627534381.16.06291100半胱氨酸合成酶（野草莓

27、）eugenol synthase 2 Fragaria x ananassagi|54006609934507.16.629699.998丁香酚合成酶2（草莓）chitinase-like protein LA-a, partial Morus albagi|353677822450946.28117100几丁酶（桑）PREDICTED: thaumatin-like protein-like Fragaria vesca subsp. vescagi|47013792325581.88.327599.724类甜蛋白基因(野草莓)PREDICTED: isoflavone reductase

28、-like protein-like Fragaria vesca subsp. vescagi|47010662862652.16.679399.996类异黄酮还原酶基因蛋白（野草莓）PREDICTED: lipoxygenase homology domain-containing protein 1-like Fragaria vesca subsp. vescagi|47010358519632.54.527699.781脂氧合酶类1。LOXHD1 PREDICTED: NADP-dependent alkenal double bond reductase P2-like Fraga

29、ria vesca subsp. vescagi|47010142941644.75.38299.945 NADP-dependent烯基双键还原酶P2（野草莓）PREDICTED: S-adenosylmethionine synthase 3-like isoform 3 Fragaria vesca subsp. vescagi|47012210343396.95.5497100S-腺苷甲硫氨酸合成酶3亚型3（野草莓）PREDICTED: stromal 70 kDa heat shock-related protein, chloroplastic-like Fragaria vesc

30、a subsp. vescagi|47012944374291.65.13631100热应激蛋白，叶绿素间质70（野草莓）PREDICTED: poly(rC)-binding protein 1-like Fragaria vesca subsp. vescagi|470132392576264.8169100poly(rC）结合蛋白（野草莓）PREDICTED: probable mediator of RNA polymerase II transcription subunit 37e-like Fragaria vesca subsp.vescagi|47013373571279.3

31、5.11708100RNA聚合酶II介质转录亚基（野草莓）retrotransposon protein, putative, Ty3-gypsy subclassgi|77556709150106.68.747496.213反转录转座子蛋白PREDICTED: LOW QUALITY PROTEIN: probable histone-lysine N-methyltransferase ATXR3-like Fragaria vesca subsp. vescagi|470102953274074.56.636193.378组蛋白赖氨酸甲基转移酶（野草莓）PREDICTED: polyadenylate-binding protein RBP45-like isoform 1 Fragaria vesca subsp. vescagi|47011640246768.35.68123100多聚腺苷酸结合蛋白 RBP45putative glycine-rich RNA-binding protein Prunus aviumgi|3485112417373.97.82101100富含甘氨酸RNA结合蛋白（樱桃）PREDICTED: nascent polypeptide-associated complex subunit alpha-like protein-li