大叶水团花茎枝的化学成分研究

1、 第 35 卷第 10 期 2010 年 5 月 Vol135, Issue10 May, 2010 Chem ica l con stituen ts of stem s and branches of A d i na po lycepha la ZHANG Yanling, GAN M aoluo, L I Shuai, WANG Sujuan, ZHU Chenggen, YANG Yongchun, HU J infeng, CHEN Naihong, SH I J iangong 3 ( Key L abora tory of B ioactive S ubstances and

2、R esou rces U tiliza tion of Ch inese Herba l M ed icine, Institu te of M a teria M ed ica, Ch inese A cadem y of M ed ica l S ciences and Peking U n ion M ed ica l College, B eijing 100050, Ch ina Abstract O bjective: To investigate chem ical constituents of the stem s and branches of A d ina polyc

3、epha la and their pharmaco2 logical activities . M ethod: The constituents were isolated by a combination of various chromatographic techniques including column chromatography on silica gel, Sephadex LH 2 20, and C 2 18, as well as reversed 2 phase HPLC. Structures of the isolates were identified by

4、 spectroscop ic data analysis . In vitro cytotoxic, anti2inflammatory, anti2 oxidant, anti2 HI V , neurop rotective and anti2diabetic activi2 ties were screened by using cell2based models . Result: Twenty2eight constituents were isolated. Their structures were identified as 2 clemochinenoside B ( 1

5、, kelampayoside A ( 2 , osmanthuside H ( 3 , 4 2hydroxy2 32 methoxyphenol2 D 2 6 2 O 2( 4 2hydroxy2 3, 5 2dim e2 thoxylbenzoate 2glucopyranoside ( 4 , and syringic acid 2 D 2glucopyranosyl ester ( 5 . Ten iridoidal glycosides: geniposidic acid ( 6 , geniposide ( 7 , 6 2hydroxygeniposide ( 8 , 6 2hyd

6、roxygeniposide ( 9 , ixoside ( 10 , ixoside 11 2 methyl ester ( 11 , 11 2 meth2 2hydroxysp lendoside ( 13 , gardoside ( 14 and mussaenosidic acid ( 15 , ( + 2 yl forsythide ( 12 , 7 p inoresinol ( 16 , ( + 2 med2 ioresinol ( 17 , ( + 2syringaresinol ( 18 , ( - 2lariciresinol ( 19 , evofolin2 B ( 20

7、, 2hydroxyacetovaillone ( 21 , syringic acid ( 22 , vanillin ( 23 , 3, 4, 5 2trim ethoxyphenol ( 24 , and 2, 6 2dim ethoxy2 1, 4 2benzoquinone ( 25 , 2sitosterol ( 26 , mannitol -5 -1 ( 27 , and daucosterol ( 28 . A t a concentration of 110 × 10 molL , these compounds were inactive in the assay

8、s, including cy2 totoxicity against human tumor cell lines ( HCT2 8, Bel2 7402, BGC 2 823, A549 and A2780 , anti2inflammatory activity against the re2 lease of2glucuronidase in rat polymorphonuclear leukocytes ( PMN s induced by p latelet2activating factor ( PAF , antioxidant activity in Fe 2+ 2cyst

9、ine2induced rat liver m icrosomal lip id peroxidation, anti2 HI V activity against H I V2 1 rep lication, neurop rotective activity 13 against serum dep rivation or glutamate induced neurotoxicity in cultures of PC12 cells, and the inhibitory activity against p rotein tyro2 sine phosphatase 1B ( PTP1B . Conclusion: Compounds 1 2 20 were obtained from the genus A d ina for the first tim e. The Key words Rubiaceae; A d ina spp; A d ina polycepha la; chem ical constituents; activity screening C2 NMR data of compounds 10 and