牛磺酸对大鼠肢体缺血再灌注后肝脏损伤的保护效应

1、牛磺酸对大鼠肢体缺血再灌注后肝脏损伤的保护效应            牛磺酸对大鼠肢体缺血再灌注后肝脏损伤的保护效应    2008-5-9 12:13:17                       

2、60;                                作者：王银环，董淑云，张娜，赵利军，李宏杰，孔小燕，刘丽华，张连元     【关键词】  再灌注损伤；肝功能；四肢；牛磺酸    Prote

3、ctive effect of taurine against liver injury after limb ischemiareperfusion in rats    【Abstract】 AIM: To observe the liver injury and investigate the protective  effect of taurine on the liver function after limb ischemiareperfusion.  METHODS: Thirty Wistar rats were r

4、andomly divided into control group，ischemiareperfusion(IR) group and taurine + ischemiareperfusion (TR) group (n=10 per group). The values of XOD, LDH, MDA, AST, ALT and SOD in plasma were respectively measured after 4 h limb ischemia  and 4 h reperfusion  in each group. XOD, MDA, ROS, MPO

5、 and Ca2+ in the liver tissues and GSHPX, Ca2+ in the liver mitochondria were observed. RESULTS:  In IR group compared with the control group, the values of LDH (190.16±13.36) kat/L vs (122.39±14.87) kat/L, XOD (758.82±151.53)  nkat/L vs (543.61±43.51) nkat/L, MDA (5.19

6、±0.67) nmol/L vs  (1.27±0.21) nmol/L , ALT (78.40±6.45) nkat/L vs  (20.50±3.70)  nkat/L, AST (47.70±4.47) nkat/L vs (25.25±2.98) nkat/L  in plasma increased, but SOD (1.30±0.15) kat/L vs (1.80±0.16) kat/L   reduced, XOD (104.69±12

7、.34) kat/kg vs (59.01±10.50) kat/kg, MDA (2.66±0.08) nmol/L vs (1.29±0.14) nmol/L, ROS (771.65±100.69) kat/L vs (606.45±52.01) kat/L, MPO (0.47±0.04 vs 0.28±0.06), Ca2+(0.248±0.050) mmol/L vs (0.123±0.014) mmol/L in the liver tissues increased, GSHPx (20.

8、34±4.67) nkat/L vs (31.17±11.50) nkat/L in the liver mitochondria  were obviously reduced, but Ca2+(0.38±0.06) mmol/L vs (0.14±0.03) mmol/L increased; In TR group compared with the IR group, the values of LDH (158.29±4.87) kat/L, XOD (758.82±151.53)  nkat/L, M

9、DA (2.81±0.19)  nmol/L, ALT (64.40±9.05)  nkat/L, AST (38.70±8.10) nkat/L were  reduced, but SOD (1.50±0.17)  kat/L in plasma of TR group increased;  the values of XOD (94.19±13.50) kat/L, MDA (1.67±0.12) nmol/L, ROS (710.81±55.34) kat/ L,

10、MPO (0.36±0.04), Ca2+ (0.192±0.426)  mmol/L in the liver tissues decreased, the values of GSHPx(22.50±3.17)  nkat/L in the liver mitochondria of TR group  obviously increased, but Ca2+ (0.31±0.06) mmol/L reduced. CONCLUSION: The taurine could protect the liver func

11、tion of  rats after limb ischemiareperfusion.    【Keywords】 reperfusion injury; liver function; extremities; taurine    【摘要】目的： 观察大鼠肢体缺血再灌注后肝脏的损伤性变化，以及牛磺酸对肝脏损伤性变化的保护效应，探讨牛磺酸对大鼠肢体缺血再灌注后肝脏功能保护作用的可能机制. 方法： 实验用Wistar大鼠30只，随机分为对照(control, C)组，缺血再灌注(ischemia re

12、perfusion, IR)组和牛磺酸+缺血再灌注(taurine+ ischemia reperfusion, TR)组，每组10只. 观察缺血4 h再灌注4 h各组大鼠血浆中XOD, LDH, MDA, AST, ALT和SOD的变化；观察肝组织XOD, MDA, ROS, MPO和Ca2+的变化；观察肝线粒体GSHPX和Ca2+的变化. 结果： 单纯肢体缺血再灌注组大鼠血浆中LDH (190.16±13.36) kat/L,  XOD (758.82±151.53) nkat/L, MDA (5.19±0.67) nmol/L, ALT (78.4

13、0±6.45) nkat/L, AST (47.70±4.47) nkat/L 等较正常对照组血浆中LDH(122.39±14.87) kat/L, XOD(543.61±43.51) kat/kg, MDA(1.27±0.21) nmol/L, ALT(20.50±3.70) nkat/L, AST(25.25±2.98) nkat/L明显增加，而SOD（1.30±0.15) kat/L较对照组 (1.80±0.16) kat/L明显降低；单纯肢体缺血再灌注组大鼠肝组织XOD (104.69±

14、12.34) kat/Kg, MDA (2.66±0.08) nmol/L, ROS (771.65±100.69) kat/ L, MPO (0.47±0.04), Ca2+ (0.248±0.050) mmol/L较正常对照组肝组织XOD(59.01±10.50) kat/kg, MDA (1.29±0.14) nmol/L,  ROS (606.45±52.01) kat/L, MPO(0.28±0.06), Ca2+ (0.123±0.014) mmol/L 均明显增加；缺血再灌注组大鼠

15、肝线粒体GSHPx活性(20.34±4.67) nkat/L与正常对照组(31.17±11.50) nkat/L比较明显降低，而Ca2+浓度(0.38±0.06) mmol/L则高于正常对照组(0.14±0.03) mmol/L. 牛磺酸+缺血再灌注组大鼠血浆中LDH(158.29±4.87) kat/L, XOD (758.82±151.53)  nkat/L, MDA (2.81±0.19) nmol/L, ALT (64.40±9.05) nkat/L, AST (38.70±8.10)

16、nkat/L较单纯缺血再灌注组明显降低，而SOD (1.50±0.17) kat/L则增加；肝组织XOD(94.19±13.50) kat/L,  MDA (1.67±0.12) nmol/L, ROS (710.81±55.34) kat/L,  MPO (0.36±0.04), Ca2+ (0.192±0.426) mmol/L等指标也较单纯缺血再灌注组明显降低，此外牛磺酸+缺血再灌注组大鼠肝线粒体GSHPx活性(22.50±3.17) nkat/L与单纯肢体缺血再灌注组相比明显增加，而Ca2+浓度(

17、0.31±0.06) mmol/L则降低，损伤减轻. 结论： 牛磺酸可以减轻大鼠肢体缺血4 h再灌注4 h后所致的肝损伤.    【关键词】 再灌注损伤；肝功能；四肢；牛磺酸    0引言    牛磺酸是体内含量最丰富的氨基酸，具有广泛的生物学作用. 已有很多实验资料2-6证明牛磺酸具有清除自由基和抗脂质过氧化作用等. 本实验我们在大鼠肢体缺血再灌注模型上观察肝脏的损伤性变化以及观察预先给予牛磺酸对这一变化的影响，旨在探讨肢体缺血再灌注时远隔器官损伤及其可能的发生机制，以及牛磺酸的保护效应，为避免或延缓肢体缺血再灌注损伤的发生、发展提供理论依据.    1材料和方法    1.1材料健康雄性Wistar大鼠30只，体质量（250±50）g，购自河南医科大