常用缓冲溶液的配制和PH计校正溶液配置方法之欧阳历创编

1、常用缓冲溶液的配制方法时间：创作人：欧阳历1.甘氨酸盐酸缓冲液(0.05mol/L)X毫升0.2 mol /L甘氨酸+Y毫升0.2 mol/L HCL再加 水稀释至200毫升PHXYPHXY2.05()44.03.05011.42.45032.43.2508.22.65024.23.4506.42.85()16.S3.65()5.0甘氨酸分子量二75.07, 0.2mol/L甘氨酸溶液含15.01克/升2.邻苯二甲酸盐酸缓冲液(0.05 mol/L)X毫升0.2 mol/L邻苯二甲酸氢钾+ 0.2 mol/L HC1,再加水稀释到20毫升pH(20C)XYpH(20 °)XY2.25

2、4.0703.251.4702.453.9603.450.9902.653.2953.650.5972.852.6423.850.2633.052.022邻苯二甲酸氢钾分子量二 204.23, 0.2 mol/L邻苯二 甲酸氢溶液含40.85克/升3?磷酸氢二钠柠檬酸缓冲液PH0.2n iol/LNa2HPO4(亳升)0.1 mol/L 柠椽酸(亳升)PH0.2mol/LNa2HPO4(亳升)0mol/L柠檬酸 (亳升)2.2Oft10.605.210.729.282.41.241&765.411.158.852.62817.825.611.608.402.83.1716.835.81

3、2.097.913.04.1115.896.012.637373.24.9415.066.213.226.783.45.7014306.413.85653.66.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.473.534.28.2811.727.217392.614.48.8211.187.418.171.834.69.3510.657.618.731.274.89.8610.147.819.150.855.010.309.7()&019.450.55Na2HP04分子量二14.98, 0.2 mol/L 溶

4、液为 28.40 克/Na2HP04? 2H2O 分子量=178.05,02 mol/L 溶液含 35.01C4H207-H20 分子量=210.14, 0.1 mol/L 溶液为 21.01克/升。4.柠檬酸氢氧化钠盐酸缓冲液PH钠离子浓度mol/L柠掾酸克C6H8O7H2O氢氧化钠克NaOH 97%盐酸亳升HC1 浓最终体积升2.20.20210841601030.2021083116103.30.202108310610430.202108345105.303524514468105.80.45285186105106.50.3826615612610 使用时可以每升中参加1克克酚，假设

5、最后pH值有变化，再用少量50%氢氧化钠溶液或浓盐酸调节，冰 箱保 存。PH0.1 mol/L 柠檬酸亳升0.1 mol/L柠檬酸钠亳升PH0.1 mol/L 柠檬酸亳升0.1 mol/L柠檬酸钠亳升3.01&61.45.08.211.83.217.22.85.27312.73.416.04.05.46.413.63.614.955.65.514.53.814.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.4&66.42.018.04.610.39.76.61.418.64.89.2KI.S柠檬酸C6H8

6、O7-H2O：分子量210.14, 0.1 mol/L溶液为21.01 克 / 升。柠檬酸钠 Na3 C6H5O7-2H2O：分子量 294.12, 0.1 mol/L 溶液为29.41克/毫升。6.乙酸乙酸钠缓冲液 0.2 mol/LpH 18°C0.2 mol/LNaAc亳升0.3 mol/LHAc亳升pH 18 °C0.2 mol/LNaAc亳升0.3 mol/LHAc亳升2.60.759.254.85.904.103.81.208.805.07.003.004.01.808.205.27.902.104.22.657355.4&601.404.43.7063

7、05.69.100.904.64.9 5.105.89.400.60Na2Ac-3H2O 分子量=136.09, 0.2 mol/L 溶液为 27.22 克/ 升7 ?磷酸盐缓冲液1磷酸氢二钠磷酸二氢钠缓冲液0.20.2 mol/L0.2 mol/L0.2 mol/L0.2 mol/LPHNa2HPO4NaH2PO4PHNa2HPO4NaH2PO4亳升毫升亳升亳升5.88.092.07.061.039.05.910.090.07.167.033.06.012.387.77.272.028.0615.085.07377.023.06.21&581.57.481.019.06322.577

8、.57.584.016.06.426.573.57.687.013.06.531.56&57.789.510.56.637.562.57.891.58.56.743.556.57.993.07.06.849.551.0&094.75.36.955.()45.0Na2HP04 - 2H20 分子量=178.05,02 mol/L 溶液为 85.61 克/Na2HP04-12H20 分子量二 358.22, 0.2 mol/L 溶液为71.64 克 / 升。Na2HP04 - 2H20 分子量=156.03,0.2 mol/L 溶液为 31.21 克/升。2磷酸氢二钠磷酸二氢钾缓冲

9、液1/15 mol/LPHmol/L/15Na2HPOmol/L/15KH2PO4(亳升)PHmol/L/15Na2HPOmol/L/15KH2PO4(亳升)4毫升4毫升4.92009.90777.003.005.290.509.507.38&002.005.911.009.007.739.001.006.242.00&00&049.500.506.473.007.00&349.750.256.644.006.00&679.90006.815.005.008.1810.0006.986.0 ()4.00Na2HPO4-2H20 分子量=178.05, 1

10、/15M 溶液为 11.876KH2P04 分子量二 136.09，1/15M 溶液为 9.078 克/ 升。?磷酸二氢钾氢氧化钠缓冲液0.05MX毫升0.2M K2P04 + Y毫升0.2N NaOH加水稀释至29毫升pH(20C)X 毫升Y 亳升pH(20 °)X 亳升Y 亳升5.850.3727.052.9636.050.5707.253.5006.250.8607.453.9506.451.2607.654.2806.651.7807.854.5206.852.365s.o54.6809.巴比妥钠盐酸缓冲液 18 °CPH0.04M巴比妥钠溶液0.2V盐酸PH0.0

11、4M巴比妥0.2N盐酸亳升钠溶液亳升6.81001&48.41005.217.010017.8&61003.827.210016.78.81002.527.41001539.01001.657.610013.49.21007.810011.479.41000.708.01009399.61000.358.210()7.21100巴比妥钠盐分子量=206.1 &0.04M溶液为8.25克/升10. Tris 盐酸缓冲液(0.05M, 25 C)50毫升0.1M三疑甲基氨基甲烷TMs溶液与X毫升0.1N盐酸混匀后，加水稀释至100毫升PHX 亳升PHX 亳升7.1045.7

12、8.1026.27.2044.7&2022.97.3043.48.3019.97.4042.0&4017.27.5040.38.5014.77.603&5&6012.47.7036.6&701037.8034.58.80&57.9032.()X.907.08.0029.2三疑甲基氨基甲烷 Tris H0CH2 CH20HCH0CH2 NH2分子量二121.14;0.1M溶液为12.114克/升。Tris溶液可从空气中吸收二氧 化碳，使用时注意将瓶盖严。11.硼酸硼砂缓冲液0.2M硼酸根PH0.05M硼砂亳升0.2M硼砂亳升PH0.05M硼砂亳升0

13、.2M硼酸亳升7.41.()9.08.23.56.57.61.58.58.44.55.57.82.0&08.76.04.08.03.07.05)S.02.0硼砂 Na2B407? H20,分子量=381.43;0.05M 溶液(=0.2M 硼酸 根)硼砂含19.07克/升硼酸H2BO3，分子量二61.84,0.2M溶液为12.37克/升。易失去结晶水，必须在带塞的瓶中保存。硼酸硼砂缓冲液0.2M硼酸根PH0.05M硼砂亳升0.2M硼酸毫升PH0.05M硼砂亳升0.2M硼酸亳升7.41.09.08.23.56.57.61.58.5&44.55.57.82.0&08.76.

14、04.08.03.07.()9.()S.02.0硼砂 Na2B407? 10H20,分子量=381,43;0.05M 溶液(二 0.2M 硼 酸根含 19.07 克/ 升。硼酸H2BO3,分子量=61.84, 0.2M溶液为12.37克/升。硼砂易失去结晶水，必须在带塞的瓶中保存12.甘氨酸氢氧化钠缓冲液 0.05MX毫升0.2M甘氨酸+Y毫升0.2Nna0H加水稀释至200毫升PHXYPHXY8.6504.09.65()22.48.8506.09.85027.29.0508.810.05032.09.25012.010.4503&69.45016.S10.65045.5甘氨酸分子量二

15、75? 07;0.2M溶液含15.01克/升13.硼砂氢氧化钠缓冲液0.05M硼酸根X毫升0.05M硼砂+Y毫升0.2NNa0H加水稀释至200毫升PHXYPHXY9.35()6.09.85()34.09.45011.010.05043.09.65()23.01().15046.0硼砂 Na2B407? 10H2O,分子量二 381.43;0.05M 溶液为 19.07 克14?碳酸钠碳酸氢钠缓冲液 0.1MCa2+、驱2+存在时不得使用PH0.1 MNa2CO3(亳升)0MN2HCO3 亳升20 C37 C969.409.519.789.9010.1410.2810.5310.838.77

16、929.409.509.729.9010.0810.2810.57123456789987654321Na2CO2?10H2O 分子量二 286.2;0.1 M 溶液为 28.62 克/ 升。N2HCO3分子量二84,0;0.1 M溶液为8? 40克/升15? PBS缓冲液PH7.67.47.27.0H2O100010001000100mlNaCl8.58.58.5&5gNa2HPO42.22.22.22? 2gNaH2PO4().10.20.30.4gPH计校正溶液配置的标准方法1) pH4,邻苯二甲酸氢钾标准缓冲液：精密称取在115±5°C枯燥2? 3小时的邻苯二甲酸氢钾1000mloKHC8H40410.12g,加水使溶解并稀释至2) pH7,磷酸盐标准缓冲液(pH7.4):精密称取在115&