荧光染料激发和发射光谱表

1、荧光素名称激发波长 (nm)发射波长 (nm)备注1,5 IAEDANS3364901,8-ANS3724804-Methylumbelliferone3855025-carboxy-2,7-dichlorofluorescein5045295-Carboxyfluorescein (5-FAM)4925185-Carboxynapthofluorescein (pH 10)512/598563/668Ratio Dye, pH5-Carboxytetramethylrhodamine (5-TAMRA)5425685-FAM (5-Carboxyfluorescein)4925185-HAT

2、 (Hydroxy Tryptamine)370-415520-5405-Hydroxy Tryptamine (HAT)370-415520-5405-ROX (carboxy-X-rhodamine)5786045 56 67 75915-TAMRA (5-Carboxytetramethylrhodamine)5485525 54 42 25686-Carboxyrhodamine 6G5185436-CR 6G5185436-JOE5205487-Amino-4-methylcoumarin3514307-Aminoactinomycin D (7-AAD)5466477-Hydrox

3、y-4-methylcoumarin360 449, 4559-Amino-6-chloro-2-methoxyacridine412, 430471, 474ABQ344445Acid Fuchsin540630ACMA (9-Amino-6-chloro-2-methoxyacridine)412, 430471, 474Acridine Orange + DNA502526Acridine Orange + RNA460650Acridine Orange, both DNA & RNA440-480520-650Acridine Red455-600560-680Acridin

4、e Yellow470550Acriflavin436520Acriflavin Feulgen SITSA355-425460Aequorin (Photoprotein)466 PhotoproteinAFPs - AutoFluorescent Protein - (Quantum Biotechnologies) see sgGFP, sgBFPAlexa Fluor 3503464423 34 42 2441Alexa Fluor 430431540Alexa Fluor 488495, 492519, 520Alexa Fluor 532531, 532553, 554Alexa

5、Fluor 546556, 557572, 573Alexa Fluor 568577, 578603Alexa Fluor 594590, 594617, 618Alexa Fluor 633632650Alexa Fluor 647647666Alexa Fluor 660668698Alexa Fluor 680679702Alizarin Complexon530-560, 580580, 624-645Alizarin Red530-560580Allophycocyanin (APC)630, 645655, 660AMC, AMCA-S345445AMCA (Aminomethy

6、lcoumarin)3454253 34 47 7444AMCA-X353442Aminoactinomycin D555655Aminocoumarin3464423 35 50 0445Aminomethylcoumarin (AMCA)3454253 34 47 7444Anilin Blue600Anthrocyl stearate360-381446APC (Allophycocyanin)630, 645655, 660APC-Cy7625-650755APTRA-BTC = Ratio Dye, Zn2+466/380520/530Ratio Dye, Zn2+APTS42450

7、5Astrazon Brilliant Red 4G500585Astrazon Orange R470540Astrazon Red 6B520595Astrazon Yellow 7 GLL450480Atabrine436490ATTO-TAG CBQCA465560ATTO-TAG FQ486591Auramine460550Aurophosphine G450580Aurophosphine450-490515BAO 9 (Bisaminophenyloxadiazole)365395BCECF (high pH)492, 503520,528BCECF (low pH)482520

8、Berberine Sulphate430550Beta Lactamase409 447, 520BFP blue shifted GFP (Y66H)381, 382, 383445, 447, 448blue shifted GFP (Y66H)Blue Fluorescent ProteinBlue Fluorescent ProteinBFP/GFP FRETBimane398490Bisbenzamide360461Bisbenzimide (Hoechst)360461bis-BTC = Ratio Dye, Zn2+455/405529/505Ratio Dye, Zn2+Bl

9、ancophor FFG390470Blancophor SV370435BOBO -1462481BOBO -3570602Bodipy 492/515490515Bodipy 493/503533549Bodipy 500/510509515Bodipy 505/515502510Bodipy 530/550528547Bodipy 542/563543563Bodipy 558/568558569Bodipy 564/570564570Bodipy 576/589579590Bodipy 581/591584592Bodipy 630/650-X625642Bodipy 650/665-

10、X647665Bodipy 665/676605676Bodipy Fl504, 505511, 513Bodipy FL ATP505514Bodipy Fl-Ceramide505511Bodipy R6G SE528547Bodipy TMR542574Bodipy TMR-X conjugate544573Bodipy TMR-X, SE544570Bodipy TR589617Bodipy TR ATP591620Bodipy TR-X SE588616BO-PRO -1462481BO-PRO -3544570Brilliant Sulphoflavin FF430520BTC -

11、 Ratio Dye Ca2+464/401533/529Ratio Dye Ca2+BTC-5N - atio Dye, Zn2+459/417517/532Ratio Dye, Zn2+Calcein494517Calcein Blue373440Calcium Crimson588, 589611, 615Calcium Green501, 506531Calcium Green-1 Ca2+ Dye506531 Ca2+ DyeCalcium Green-2 Ca2+506/503536 Ca2+Calcium Green-5N Ca2+506532 Ca2+Calcium Green

12、-C18 Ca2+509530 Ca2+Calcium Orange549575576Calcofluor White385, 395, 405437, 440, 445Carboxy-X-rhodamine (5-ROX)576601Cascade Blue3774203 39 98 84233 39 99 9Cascade Yellow3995504 40 00 0552Catecholamine410470CCF2 (GeneBlazer)CFDA494520CFP - Cyan Fluorescent Protein430, 433, 436, (453)474, 475, 476,

13、(501)Cyan Fluorescent ProteinCFP/YFP FRETChlorophyll480650Chromomycin A436-460470Chromomycin A445575CL-NERF (Ratio Dye, pH)504/514540 Ratio Dye, pHCMFDA494520Coelenterazine Ca2+ Dye, bioluminescence-429465Ca2+ Dye, bioluminescence,native moleculeCoelenterazine cp (Ca2+ Dye,)-430442 Ca2+ Dye, biolumi

14、nescenceCoelenterazine f-437473 Ca2+ Dye, bioluminescenceCoelenterazine fcp452 Ca2+ Dye, bioluminescenceCoelenterazine h-437464 Ca2+ Dye, bioluminescenceCoelenterazine hcp-433444 Ca2+ Dye, bioluminescenceCoelenterazine ip441 Ca2+ Dye, bioluminescenceCoelenterazine n-431467 Ca2+ Dye, bioluminescenceC

15、oelenterazine O460575Coumarin Phalloidin387470C-phycocyanineCPM Methylcoumarin384469 MethylcoumarinCTC400-450602CTC FormazanCy2489506Cy3.1 8554568Cy3.5581598Cy35145665 55 52 25705 55 54 4Cy5.1 8649666Cy5.5675695Cy5649666670Cy7710, 743767, 805Cyan GFP433 (453)475 (501)cyclic AMP Fluorosensor (FiCRhR)

16、500517CyQuant Cell Proliferation Assay480520 Cell Proliferation AssayDabcyl453Dansyl340578Dansyl Amine337517Dansyl Cadaverine335518Dansyl Chloride372518Dansyl DHPE336517Dansyl fluoride356 noneDAPI359461Dapoxyl403580Dapoxyl 2374574Dapoxyl 3373574DCFDA504529DCFH (Dichlorodihydrofluorescein Diacetate)5

17、05535DDAO463607DHR (Dihydorhodamine 123)505534Di-4-ANEPPS496705Di-8-ANEPPS (non-ratio)4886054 49 98 8713DiA (4-Di-16-ASP)456591Dichlorodihydrofluorescein Diacetate (DCFH)505535DiD - Lipophilic Tracer644665 Lipophilic TracerDiD (DiIC18(5)644665DIDS341415Dihydorhodamine 123 (DHR)505535DiI (DiIC18(3)54

18、9, 551565Dinitrophenol349DiO (DiOC18(3)484, 487501, 502DiR748780 Lipophilic TracerDiR (DiIC18(7)750779DM-NERF (high pH)497/510540 Ratio Dye, pHDNP349Dopamine340 490-520DsRed558583 Red fluorescent proteinDTAF494520DY-630-NHS621660Hemicyanelabel for proteins and DNADY-635-NHS634664Hemicyanelabel for p

19、roteins and DNAEBFP383447 Enhanced Blue Fluorescent ProteinECFP436474 Enhanced Cyan Fluorescent ProteinEGFP488, 498507, 516Enhanced Green Fluorescent ProteinELF 97345530Eosin524545Erythrosin529, 532554, 555Erythrosin ITC529555Ethidium Bromide510, 523595, 605Ethidium homodimer -1 (EthD-1)528617Euchry

20、sin430540EukoLightEuropium (III) chlorideEYFP513, 520527, 532Enhanced Yellow Fluorescent ProteinFast Blue360440FDA494520Feulgen (Pararosaniline)570625FIF (Formaldehyd Induced Fluorescence)405433FITC490, 494520, 525FITC Antibody493517Flazo Orange375-530612Fluo-3480 506, 506520, 527Fluo-4494516Fluores

21、cein (FITC)490, 494520, 525Fluorescein Diacetate494520Fluoro-Emerald495524Fluoro-Gold (Hydroxystilbamidine)361536Fluor-Ruby555582FluorX494520FM 1-43479598FM 4-46515640Fura Red (high pH)572657Fura Red/Fluo-3Fura-2, high calcium335505 Excitation ratio dyeFura-2, low calcium363512 Excitation ratio dyeF

22、ura-2/BCECFGenacryl Brilliant Red B520590Genacryl Brilliant Yellow 10GF430485Genacryl Pink 3G470583Genacryl Yellow 5GF430475GeneBlazer (CCF2)GFP (S65T)498516GFP red shifted (rsGFP)498516GFP wild type, non-UV excitation (wtGFP)475509GFP wild type, UV excitation (wtGFP)395509GFPuv385508Gloxalic Acid40

23、5460Granular Blue355425Haematoporphyrin530-560580Hoechst 33258345487Hoechst 33342347483Hoechst 34580392440HPTS355465Hydroxycoumarin325-360386-455Hydroxystilbamidine (FluoroGold)361536Hydroxytryptamine400530Indo-1, high calcium330401 Emission ratio dyeIndo-1, low calcium346475 Emission ratio dyeIndod

24、icarbocyanine (DiD)644665Indotricarbocyanine (DiR)748780Intrawhite Cf360430JC-1514529JO-JO-1530545JO-PRO-1532544LaserPro795812Laurodan355460LDS 751 (DNA)543712LDS 751 (RNA)590607Leucophor PAF370430Leucophor SF380465Leucophor WS395465Lissamine Rhodamine572, 577591, 592Lissamine Rhodamine B577592LIVE/

25、DEAD Kit Animal Cells494517 for more details referCalcein/Ethidium homodimer528617 to LOLO-1566580LO-PRO-1568581Lucifer Yellow425, 428528, 536, 540Lyso Tracker Blue373422Lyso Tracker Blue-White466536Lyso Tracker Green504, 534511, 551Lyso Tracker Red490516Lyso Tracker Yellow551576LysoSensor Blue37442

26、4LysoSensor Green442505LysoSensor Yellow/Blue384540Mag Green507531Magdala Red (Phloxin B)524600Mag-Fura Red483/427659/631Ratio Dye, Mg2+Mag-Fura-2369/329508 Ratio Dye Ca2+369/330511/491Ratio Dye Mg2+Mag-Fura-5369/330505/500Ratio Dye, Ca2+369/332505/482Ratio Dye, Mg2+Mag-Indo-1349/328480/390Ratio Dye

27、, Ca2+349/330480/417Ratio Dye, Mg2+Magnesium Green506, 507531Magnesium Orange550575Malachite Green628Marina Blue362459Maxilon Brilliant Flavin 10 GFF450495Maxilon Brilliant Flavin 8 GFF460495Merocyanin555578Methoxycoumarin360410Mitotracker Green FM490516Mitotracker Orange551576Mitotracker Red578599M

28、itramycin450470Monobromobimane398490Monobromobimane (mBBr-GSH)398500Monochlorobimane380461MPS (Methyl Green Pyronine Stilbene)364395NBD466539NBD Amine450530Nile Red515-555, 559590, 640Nitrobenzoxadidole465 510-650Noradrenaline340 490-520Nuclear Fast Red289-530580Nuclear Yellow365495Nylosan Brilliant

29、 Iavin E8G460510Oregon Green503522Oregon Green 488-X494517Oregon Green503522Oregon Green 488490, 493514, 520Oregon Green 500497517Oregon Green 514506526Pacific Blue405455Pararosaniline (Feulgen)570625PBFI340/380420 Excitation ratio dyePE-Cy5488670PE-Cy7488 755, 767PerCP488675PerCP-Cy5.5488710PE-Texa

30、sRed Red 613488613Phloxin B (Magdala Red)524600Phorwite AR360430Phorwite BKL370430Phorwite Rev380430Phorwite RPA375430Phosphine 3R465565PhotoResist365610Phycoerythrin B PE546-565575Phycoerythrin R PE565578PKH26 (Sigma)551567PKH67496520 ChromaPMIA341376Pontochrome Blue Black535-553605POPO-1433457POPO

31、-3533574PO-PRO-1435455PO-PRO-3539567Primuline410550Procion Yellow470600Propidium Iodid (PI)(305), 536, 538617PyMPO412, 415561, 564, 570Pyrene360387Pyronine410540Pyronine B540-590560-650Pyrozal Brilliant Flavin 7GF365495QSY 7560Quinacrine Mustard440510Red 613 PE-TexasRed488613Resorufin571 584, 585RH

32、414532716Rhod-2552576Rhodamine550573Rhodamine 110496, 497520Rhodamine 123507529Rhodamine 5 GLD470565Rhodamine 6G525555Rhodamine B540625Rhodamine B 200523-557595Rhodamine B extra550605Rhodamine BB540580Rhodamine BG540572Rhodamine Green502527Rhodamine Phallicidine558, 542575, 565Rhodamine Phalloidine5

33、42565Rhodamine Red570590Rhodamine WT530555Rose Bengal525, 540550-600R-phycocyanineR-phycoerythrin (PE)565578rsGFP473509 red shifted GFP (S65T)S65A471504S65C479507S65L484510S65T488511Sapphire GFP395511SBFI340/380420 Excitation ratio dyeSerotonin365 520-540Sevron Brilliant Red 2B520595Sevron Brilliant

34、 Red 4G500583Sevron Brilliant Red B530590Sevron Orange440530Sevron Yellow L430490sgBFP387450sgBFP (super glow BFP)387450Quantums SuperGloGFPAFPssgGFP474488sgGFP (super glow GFP)474509Quantums SuperGloGFPAFPsSITS336436 Ion ChannelsSITS (Primuline)395-425450SITS (Stilbene Isothiosulphonic Acid)365460S

35、NAFL calcein506/535535/620Ratio Dye, pHSNAFL-1508/540543/623Ratio Dye, pHSNAFL-2514/543546/630Ratio Dye, pHSNARF calcein552/574590/629Ratio Dye, pHSNARF1576/548635/587Excitation and emission ratio dyeSodium Green506, 507532 Na+, K+SpectrumAqua433,/53480/55VysisSpectrumGreen497/30, 509/31538/44, 524/

36、56VysisSpectrumOrange559/38, 560588/48VysisSpectrum Red587, 587/35612, 612/51SPQ (6-methoxy-N-(3-sulfopropyl)quinolinium)344443Stilbene335440Sulphorhodamine B can C520595Sulphorhodamine G Extra470570SYTO 11508, 510527, 530Dye for DNA, RNASYTO 12499, 500522, 519Dye for DNA, RNASYTO 13488, 491509, 514

37、Dye for DNA, RNASYTO 14517, 521549, 547Dye for DNA, RNASYTO 15516, 518546, 555Dye for DNA, RNASYTO 16488, 494518, 525Dye for DNA, RNASYTO 17621634 Dye for DNASYTO 18490, 493507, 527Dye for DNA, RNASYTO 20512530 Dye for DNASYTO 21494517 Dye for DNASYTO 22515535 Dye for DNASYTO 23499520 Dye for DNASYTO 24490515 Dye for DNASYTO 25521556 Dye for DNASYTO 40420441 Dye for DNASYTO 41430454 Dye for DNASYTO 42433460 Dye for DNASYTO 43436467 Dye for DNASYTO 44446471 Dye for DNASYTO 45452484 Dye for DNASYTO 59622645 Dye fo