微藻培养过程中一氧化二氮的定量测定

1、Quantitative Measurement of Direct Nitrous Oxide Emissions from Microalgae CultivationLife Cycle Assessments (LCA) of microalgae-based biofuels have suggested net reductions of greenhouse gas emissionsa kind of potent greenhouse gasisnt considered in LCA BACKGROUNDMETHODS AND METERIALSCultivate the

2、microalgae with light-dark cycling213Simulate PBR (photobioreactor)and ORP(open raceway pond)conditionsUsing Fourier transform infrared(FTRI) mearsure N2O quantitativelyMicroalgae species Nannochloropsis salina (1776)4DNA extraction andPCR amplificationRESULTSORP Condition The total emission mass of

3、 N2O 2.3510-5kgORP ConditionRESULTSPBR Condition The total emission mass of N2O4.3810-4kg PBR ConditionN2O production route？Hypothesis：RESULTSDenitrifying BacterianorB genes was detected in the experiment culturesencoding for nitric oxide reductase, which catalyzes the reduction of NO to N2ON2O emis

4、sions observed in these studies are a byproduct of the anoxic bacterial denitrification pathwayRESULTSSUMMARY1.Using FTIR measure the N2Oemission, get the quantitative date of N2O emmision4.N2O emissions are a byproduct of the anoxic bacterial denitrification pathway5.Based on the data presented in this study, the LCAfor microalgae LCA in the future must consider the potential for direct N2O emissions from the cultivation stage2.Light periods were significantly lower than the emissions for the dark periods both in PBR and ORP condition3.Cumulative N2O emissions in the PBR is substantially h