限制性酶的酶切位点

1、各种限制性酶的酶切位点Enzyme Name Sequence AatI AGG!CCT AatII GACGT!C AccI GT!MKAC AccII CG!CG AccIII T!CCGGA AcyI GR!CGYC AflI G!GWCC AflII C!TTAAG AflIII A!CRYGT AhaI CC!SGG AhaII GR!CGYC AhaIII TTT!AAA AluI AG!CT AlwI GGATCNNNN! AlwI !NNNNNGATCC AlwNI CAGNNN!CTG AocI CC!TNAGG AocII GDGCH!C AosI TGC!GCA AosI

2、I GR!CGYC ApaI GGGCC!C ApaLI G!TGCAC ApyI CC!WGG AquI C!YCGRG AseI AT!TAAT AspI GAANN!NNTTC AspI G!GTACC AspI GACN!NNGTC AsuI G!GNCC AsuII TT!CGAA AvaI C!YCGRG AvaII G!GWCC AvaIII ATGCA!T AvrI !CYCGRG AvrII C!CTAGG AxyI CC!TNAGG BalI TGG!CCA BamHI G!GATCC BanI G!GYRCC BanII GRGCY!C BanIII AT!CGAT Bb

3、eI GGCGC!C BbiII/AcyI GR!CGYC BbvI GCAGCNNNNNNNN! BbvI !NNNNNNNNNNNNGCTGC BbvII GAAGACNN! BbvII !NNNNNNGTCTTC BcefI ACGGCNNNNNNNNNNNN! BcefI !NNNNNNNNNNNNNGCCGT BclI T!GATCA BcnI CC!SGG BglI GCCNNNN!NGGC BglII A!GATCT BinI !NNNNNGATCC BinI GGATCNNNN! BsePI !GCGCGC BsmAI !GTCTC BsmAI !GAGAC BsmI GAAT

4、GCN! BsmI G!CATTC BspI GDGCH!C BspHI T!CATGA BspMI ACCTGCNNNN! BspMI !NNNNNNNNGCAGGT BspMII T!CCGGA BsrI ACTGGN! BsrI C!CAGT BssHII G!CGCGC BstBI TT!CGAA BstEII G!GTNACC BstI G!GATCC BstNI CC!WGG BstPI G!GTNACC BstUI CG!CG BstXI CCANNNNN!NTGG BstYI R!GATCY BsuI CC!TNAGG CcrI C!TCGAG CfoI GCG!C CfrI

5、R!CCGGY CfrI G!GNCC CfrI Y!GGCCR ClaI AT!CGAT CviJI RG!CY CvnI CC!TNAGG DdeI C!TNAG DpnI GA!TC DraI TTT!AAA DraII RG!GNCCY DraIII CACNNN!GTG DsaI C!CRYGG EaeI Y!GGCCR EagI C!GGCCG EarI !CTCTTC EarI !GAAGAG EclXI C!GGCCG EcoI TAC!GTA EcoI GGTCTCN! EcoI !NNNNNGAGACC EcoI G!GWCC EcoIII AGC!GCT EcoI C!G

6、GCCG EcoI !CTGAAG EcoI !CTTCAG EcoI CC!TNAGG EcoNI CCTNN!NNNAGG EcoOI RG!GNCCY EcoRI G!AATTC EcoRII !CCWGG EcoRV GAT!ATC EcoTI C!CWWGG EcoTI ATGCA!T EcoTI GRGCY!C EheI GGC!GCC EspI GC!TNAGC FinI !GTCCC FinI !GGGAC FnuHI GC!NGC FokI GGATGNNNNNNNNN! FokI !NNNNNNNNNNNNNCATCC FspI TGC!GCA GdiII !NNNNNYG

7、GCCG GdiII CGGCCRN! GsuI !CTCCAG GsuI !CTGGAG HaeI WGG!CCW HaeII RGCGC!Y HaeIII GG!CC HapII C!CGG HgaI GACGCNNNNN! HgaI !NNNNNNNNNNGCGTC HgiAI GWGCW!C HgiEII !ACCNNNNNNGGT HhaI GCG!C HinI GR!CGYC HinPI G!CGC HincII GTY!RAC HindIII A!AGCTT HinfI G!ANTC HpaI GTT!AAC HpaII C!CGG HphI GGTGANNNNNNNN! Hph

8、I !NNNNNNNTCACC KpnI GGTAC!C KspI CTCTTCN! KspI !NNNNGAAGAG MaeI C!TAG MaeII A!CGT MaeIII !GTNAC MboI !GATC MboII GAAGANNNNNNNN! MboII !NNNNNNNTCTTC MfeI !CAATTG MflI R!GATCY MluI A!CGCGT MmeI !TCCRAC MmeI !GTYGGA MnlI CCTCNNNNNNN! MnlI !NNNNNNNGAGG MroI T!CCGGA MseI T!TAA MspI C!CGG MstI TGC!GCA Ms

9、tII CC!TNAGG MvaI CC!WGG NaeI GCC!GGC NarI GG!CGCC NciI CC!SGG NcoI C!CATGG NdeI CA!TATG NdeII !GATC NheI G!CTAGC NlaIII CATG! NlaIV GGN!NCC NotI GC!GGCCGC NruI TCG!CGA NsiI ATGCA!T Nsp()I RCATG!Y Nsp()V TT!CGAA NspBII CMG!CKG NspII GDGCH!C NspIII C!YCGRG NspIV G!GNCC NunII GG!CGCC PaeR C!TCGAG PalI

10、 GG!CC PflMI CCANNNN!NTGG PleI GAGTCNNNN! PleI !NNNNNGACTC PmaCI CAC!GTG PpuMI RG!GWCCY PstI CTGCA!G PvuI CGAT!CG PvuII CAG!CTG RsaI GT!AC RsrI G!AATTC RsrII CG!GWCCG SacI GAGCT!C SacII CCGC!GG SalI G!TCGAC SauAI !GATC SauI G!GNCC SauI CC!TNAGG ScaI AGT!ACT ScrFI CC!NGG SduI GDGCH!C SecI C!CNNGG Sex

11、I !CTCGAG SfaNI GCATCNNNNN! SfaNI !NNNNNNNNNGATGC SfiI GGCCNNNN!NGGCC SinI G!GWCC SmaI CCC!GGG SnaBI TAC!GTA SnaI !GTATAC SpeI A!CTAGT SphI GCATG!C SplI C!GTACG SspI AAT!ATT SstI GAGCT!C SstII CCGC!GG SstIII !ACGT StuI AGG!CCT StyI C!CWWGG StySJI !GAGNNNNNNGTRC StySJI !GYACNNNNNNCTC TaqI T!CGA TaqII GACCGANNNNNNNNNNN! TaqII !NNNNNNNNNTCGGTC TaqII CACCCANNNNNNNNNNN! TaqII !NNNNNNNNNTGGGTG ThaI CG!CG TspI !GTSAC TspEI !AATT TthI GACN!NNGTC TthI