经皮植入记忆合金自体膨胀支架体外研究ppt课件

1、Implantation of Endothelial Progenitor Cells on a Self-expanding Metal Stent: an in vitro StudyHongjian Shi,MDGaojun Teng,Ph.D,MDAihong Cao,MDDeparment of Radiology, Zhongda Hospital, Southeast UniverstyContentoBackgroundoMaterials and methodsoResultsoDiscussionBackgroundoRestenosis:pseudointimal hy

2、perplasia ( PTA, stent)oHigh restenosis in TIPS(30%-50%, 1y)oBile leakage unavoidable during TIPSoBile inhibit both SMC and ECoCovered Viattor stento 1. Coldwell D, Radiology 2019o 2. Zhuang Z,AJR 2019o 3. LaBerge J,JVIR 1993o 4. Nishimine K,Radiology 2019o 5. Teng G,Radiology 2019;2019o 6. Cejna M,

3、Radiology 2019o 7. Hausegger K,JVIR 2019Background oEPC(endothelial progenitor cell) can differentiate into EC(endothelial cell) and incorporate into sites of active angiogenesis. o o Asahara, Science 2019Shantsila E, et al.JACC 2019EPC Isolation,Culture,IdentificationRepresentative relative distrib

4、ution of some of the antigens expressed inCECs, as depicted by flow cytometry. Bertolini F et al. Drug Discovery Today 2019;12:806-812Photographs of EPCEPC uptaken DiI-Ac-LDLEPC binding FITC-UEA-1Immunocytochemistry EPC cell markers CD34, KDR,vWFFibrinogen Preparationo15 ml peripheral blood from the

5、 same animaloCryoprecipitated techniqueo Centrifugation at 1250 rpm at 4 for 20 min the plasma frozen to 70, stored at -20 4 refrigerator centrifugation at 2500 rpms at 2 for 40 min o1.5ml concentrated fibriongeno DePalma L, Transfusion 1993Procedure of EPC seeding on stento10 self-expanding woven m

6、etal stents(Microtech, Nanjing): 5 in case group and 5 in control group.oFibrin gel(fibrinogen 5mg/ml+thrombin 0.1U/ml)oIncubate for 24 h (rotate every 8 h) in EGM-2 with EPC concentration 1106 /mloCell count oRinsed EPC re-identifiedo 1 Kipshidze N, JACC 2000o 2 Shirota T, Biomaterials 2019Cell cou

7、ntoRinsed cell from EPC seeded stent:o 2105DiscussionoIt is feasible to devise an EPC-seeded stent in vitrooApplicabe in TIPS as well as vascular systemoEPC-seeded stent with photocured-geloCD34 antibody capture EPC Stento 1 Shirota T, Biomaterials 2019o 2 Aoki J, JACC 2019AcknowlegementoThis study was supported by grant 30670604 of the national natural science foundation of ChinaoStents were sponsored by( Microtech Co.,Nanjing)oThanks f