各种缓冲液的配制方法大全

1、磷酸氢二钠书檬酸缓冲液0.2mol/L0.1mol/L0.2mol/L0.1mol/LPHNa2HPO4柠檬酸PHNa2HPO4柠檬酸(<#)(<#)(<#)(<#)2.20.4010.605.210.729.282.41.2418.765.411.158.852.62.1817.825.611.608.402.83.1716.835.812.097.913.04.1115.896.012.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906

2、.815.454.554.07.7112.297.016.473.534.28.2811.727.217.392.614.48.8211.187.418.171.834.69.3510.657.618.731.274.89.8610.147.819.150.855.010.309.708.019.450.55Na2HPO4分子量=14.98,0.2mol/L溶液为28.40克/升。Na2HPO42H2O分子量=178.05,0.2mol/L溶液含35.01克/升。C4H2O7-H2O分子量=210.14,0.1mol/L溶液为21.01克/升20%盐酸溶液如何配置：取浓盐酸（质量百分比浓度为3

3、6.5%）一个体积（如100毫升），加入到96.5毫开水中就可以了。克）所需水的质量为;213.5-117=96.5克，也就是96.5毫升水。磷酸盐缓冲液配制方法（PEJ）磷酸盐缓冲液(PhosphateBuffer,PB)试齐I：NaH2PO42H2ONa2HPO412H2O配制方法：配制时，常先配制0.2mol/L的NaH2Po4和0.2mol/L的Na2HPO4,两者按一定比例混合即成0.2mol/L的磷酸盐缓冲液(PB),根据需要可配制不同浓度的PB和PBS。(1)0.2mol/L的NaH2P。4；称取NaH2PO4.2H2O31.2g(或NaH2P04-H2O27.6g)加重蒸水至1

4、000ml溶解。(2) 0.2mol/L的Na2HPO4：称取Na2HPO4.12H2O71.632g(或Na2HP047H2053.6g或Na2HP042H2035.6g)加重蒸水至1000ml溶解。(3) 0.2mol/LpH7.4的PB的配制：取19ml0.2mol/L的NaH2Po4和81ml0.2mol/L的Na2HPO412H2O,充分混合即为0.2mol/L的PB(pH约为7.47.5)。若pH偏高或偏低，可通过改变二者的比例来加以调整，室温保存即可。0.2mol/L磷酸盐缓冲液(pH5.78.0)pH0.2mol/LNaH2P04(ml)0.2mol/LNa2HPO4(ml)5

5、.7 93.56.55.8 92.08.05.9 90.010.06.1 87.712.36.1 85.015.06.2 81.518.56.3 77.522.56.4 73.526.56.5 68.531.56.6 62.537.56.7 56.543.56.851.049.06.945.055.07.039.061.07.133.067.07.228.072.07.323.067.07.419.081.07.516.084.07.613.087.07.710.589.57.88.591.57.97.093.08.05.394.70.05mol/L磷酸盐缓冲液(pH7.0)如何配制?0.05

6、mol/LNaH2PO4(ml)0.05mol/LNa2HPO4(ml)5.793.56.55.892.08.05.090.010.06.G87.71236.185.015.06.281.518.56.377.522.56.473.526.56.568.531.56.662.537.56.756.543.56.851.049.06.945.055.07.039.061.07.133.067.07.228.072.07323.067.07.419.081.07.516.084.07.613.087.07.710.589.57.88.591.57.97.093.08.05.394.725工下0.1

7、m。I/L僦酸钾耍冲液的纪制pH0©5moi/LK2HPO4(ml)0.05mol/LKH2PO4(ml)5.88.59158。13-286.86.219280.86.427s72.26.638.161.96.849750.37.06L538.57.271728.37.480-219.87.686.613.47.890-8928.0946。PH3-10缓冲溶液的配制方法i.磷酸氢二钠-柠檬酸缓冲液PH0.2mol/LNa2HPO4(<#)0.1mol/L柠檬酸(<#)PH0.2mol/LNa2HPO4(<#)0.1mol/L柠檬酸(<#)2.20.4010.6

8、05.210.729.282.41.2418.765.411.158.852.62.1817.825.611.608.402.83.1716.835.812.097.913.04.1115.896.012.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.473.534.28.2811.727.217.392.614.48.8211.187.418.171.834.69.3510.657.618.731.2

9、74.89.8610.147.819.150.855.010.309.708.019.450.55Na2HPO4分子量=14.98,0.2mol/L溶液为28.40克/升。Na2HPO42H2O分子量=178.05,0.2mol/L溶液含35.01克/升。C4H2O7-hO分子量=210.14,0.1mol/L溶液为21.01克/升。2.柠檬酸-氢氧化钠-盐酸缓冲液PH钠离子浓度（mol/L）柠檬酸（克）C6H8。7-出。氢氧化钠（克）NaOH97%盐酸（毫升）HCl（浓）最终体积（升）2.20.2021084160103.10.2021083116103.30.2021083106104.3

10、0.202108345105.30.3524514468105.80.45285186105106.50.3826615612610使用时可以每升中加入1克克酚，若最后pH值有变化，再用少量50%氢氧化钠溶液或浓盐酸调节，冰箱保存。3.甘氨酸-盐酸缓冲液（0.05mol/L）X毫升0.2mol/L甘氨酸+Y毫升0.2mol/LHCI,再加水稀释至200毫升PHXYPHXY2.05044.03.05011.42.45032.43.2508.22.65024.23.4506.42.85016.83.6505.0甘氨酸分子量=75.07,0.2mol/L甘氨酸溶液含15.01克/升。4.邻苯二甲酸-

11、盐酸缓冲液（0.05mol/L）X毫升0.2mol/L邻苯二甲酸氢钾+0.2mol/LHCl,再加水稀释到20毫升pH(20C)XYpH(20C)XY2.254.0703.251.4702.453.9603.450.9902.653.2953.650.5972.852.6423.850.2633.052.022邻苯二甲酸氢钾分子量=204.23,0.2mol/L邻苯二甲酸氢溶液含40.85克/升5.柠檬酸-柠檬酸钠缓冲液（0.1mol/L）PH0.1mol/L柠檬酸0.1mol/L柠檬酸钠PH0.1mol/L柠檬酸0.1mol/L柠檬酸钠3.018.61.45.08.211.83.217.22

12、.85.27.312.73.416.04.05.46.413.63.614.95.15.65.514.53.814.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.48.66.42.018.04.610.39.76.61.418.64.89.210.8柠檬酸C6H8。7H2O：分子量210.14,0.1mol/L溶液为21.01克/升。柠檬酸钠Na3C6H5O7-2H2O:分子量294.12,0.1mol/L溶液为29.41克/升。柠檬酸C6H8。7-dO:2.1g柠檬酸钠Na3C6H5O7.2H2O:2.94gH2O:1

13、00ml6.乙酸-乙酸钠缓冲液(0.2mol/L)pH(18C)0.2mol/LNaAc(<#)0.3mol/LHAc(<#)pH(18C)0.2mol/LNaAc(<#)0.3mol/LHAc(<#)2.60.759.254.85.904.103.81.208.805.07.003.004.01.808.205.27.902.104.22.657.355.48.601.404.43.706.305.69.100.904.64.905.105.89.400.60Na2Ac3H2O分子量=136.09,0.2mol/L溶液为27.22克/升。7 .磷酸盐缓冲液(1)磷酸氢

14、二钠-磷酸二氢钠缓冲液(0.2)PH0.2mol/LNa2HPO4(<#)0.2mol/LNaH2PO4(<#)PH0.2mol/LNa2HPO4(<#)0.2mol/LNaH2PO4(<#)5.88.092.07.061.039.05.910.090.07.167.033.06.012.387.77.272.028.06.115.085.07.377.023.06.218.581.57.481.019.06.322.577.57.584.016.06.426.573.57.687.013.06.531.568.57.789.510.56.637.562.57.891.

15、58.56.743.556.57.993.07.06.849.551.08.094.75.36.955.045.0Na2HPO42H2O分子量=178.05,0.2mol/L溶液为85.61克/升。Na2HPO412H2O分子量=358.22,0.2mol/L溶液为71.64克/升。Na2HP042H2O分子量=156.03,0.2mol/L溶液为31.21克/升。（2）磷酸氢二钠-磷酸二氢钾缓冲液（1/15mol/L）PHmol/L/15Na2HPO4(<#)mol/L/15KH2PO4(<#)PHmol/L/15Na2HPO4(<#)mol/L/15KH2PO4(<

16、#)4.920.109.907.177.003.005.290.509.507.388.002.005.911.009.007.739.001.006.242.008.008.049.500.506.473.007.008.349.750.256.644.006.008.679.900.106.815.005.008.1810.0006.986.004.00Na2HP042H2O分子量=178.05,1/15M溶液为11.876克/升。KH2PO4分子量=136.09,1/15M溶液为9.078克/升。8 .磷酸二氢钾-氢氧化钠缓冲液（0.05M）X毫升0.2MK2PO4+Y毫升0.2NNaO

17、H加水稀释至29毫升pH(20C)X(<#)Y(<#)pH(20C)X(<#)Y(<#)5.850.3727.052.9636.050.5707.253.5006.250.8607.453.9506.451.2607.654.2806.651.7807.854.5206.852.3658.054.6809.巴比妥钠-盐酸缓冲液（18C）PH0.04M巴比妥钠溶液0.2V盐酸PH0.04M巴比妥钠溶液（毫升）0.2N盐酸（毫升）6.810018.48.41005.217.010017.88.61003.827.210016.78.81002.527.410015.39.0

18、1001.657.610013.49.21001.137.810011.479.41000.708.01009.399.61000.358.21007.21100巴比妥钠盐分子量=206.18;0.04M溶液为8.25克/升10.Tris-盐酸缓冲液（0.05M,25C）50毫升0.1M三羟甲基氨基甲烷（Tris）溶液与X毫升0.1N盐酸混匀后，加水稀释至100毫升。PHX(<#)PHX(<#)7.1045.78.1026.27.2044.78.2022.97.3043.48.3019.97.4042.08.4017.27.5040.38.5014.77.6038.58.6012.

19、47.7036.68.7010.37.8034.58.808.57.9032.08.907.08.0029.2三羟甲基氨基甲烷(Tris)HOCH2CH2OHCHOCH2NH2分子量=121.14;0.1M溶液为12.114克/升。Tris溶液可从空气中吸收二氧化碳，使用时注意将瓶盖严。11.硼酸-硼砂缓冲液（0.2M硼酸根）PH0.05M硼砂(<#)0.2M硼砂(<#)PH0.05M硼砂(<#)0.2M硼酸(<#)7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂Na2B

20、407H2O,分子量=381.43;0.05M溶液（=0.2M硼酸根）含19.07克/升。硼酸H2BO3,分子量=61.84,0.2M溶液为12.37克/升。硼砂易失去结晶水，必须在带塞的瓶中保存。11.硼酸-硼砂缓冲液（0.2M硼酸根）PH0.05M硼砂0.2M硼酸(<#)PH0.05M硼砂(<#)0.2M硼酸7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂Na2B40710H2O,分子量=381.43;0.05M溶液（=0.2M硼酸根）含19.07克/升。硼酸H2BO3,分子量=61.84,0.2M溶液为12.37克/升。硼砂易失去结晶水，必须在带塞的瓶中保存。12.甘氨酸-氢氧化钠缓