斑马鱼的性腺发育9

1、1会计学斑马鱼的性腺发育斑马鱼的性腺发育9SB产卵板繁殖期，IV期卵巢放入隔板及斑馬魚上盒與普通下盒組裝上盒底以網狀材料代替123490% Leibovitz L-15，pH990% Leibovitz L-15Leibovitzs Mediumspecifically designed to growncells in a CO2 free atmosphere. The standard sodium bicarbonate/ CO2 buffering system is replaced by combination of free basic amino acids, phospha

2、te buffers and higher levels of galactose and sodium pyruvate. As a result, the medium does not require supplementation with sodium bicarbonate and can be used under conditions of free gaseous exchange with the atmosphere. The medium can be used to grow human tumor cells and embryonic cells and also

3、 established cell lines like HeLa and Hep-2. The medium is frequently used in diagnostic virology where tissue cell lines or strains need to be grown in closed systems. Leibovitzs medium obviates the need of frequent medium change.Composition :nIngredients mg/LnINORGANIC SALTSnCalcium chloride dihyd

4、rate 185.000nMagnesium chloride hexahydrate 200.000nMagnesium sulphate anhydrous 97.720nPotassium chloride 400.000nPotassium phosphate, monobasic 60.000nSodium chloride 8000.000nSodium phosphate, dibasic anhydrous 190.120nAMINO ACIDSnDL-Alpha alanine 450.000nGlycine 200.000nL-Arginine (free base) 50

5、0.000nL-Asparagine 250.000nL-Cysteine (free base) 120.000nL-Glutamine 300.000nL-Histidine (free base) 250.000nL-Isoleucine 250.000nL-Leucine 125.000nL-Lysine hydrochloride 94.000nL-Methionine 75.000nL-Phenylalanine 125.000nL-Serine 200.000nL-Threonine 300.000nL-Tryptophan 20.000nL-Tyrosine Disodium

6、Salt 276.160nL-Valine 100.000nVITAMINSnCholine chloride 1.000nD-Ca-Pantothenate 1.000nFolic acid 1.000nNicotinamide 1.000nPyridoxine hydrochloride 1.000nRiboflavin-5-phosphate, Na 0.100nThiamine monophosphate 1.000ni-Inositol 2.000nOTHERSnD-Galactose 900.000nPhenol red Sodium Salt 11.000nSodium pyru

7、vate 550.000nDirections :n1. Suspend 14.0gms in 900ml tissue culture grade water with constant, gentle stirring until the powder is completelyndissolved. Do not heat the water. Note: Presence of slight haziness in the medium is due to inherent nature of some ofnthe ingredients in the composition. Ho

8、wever, this will not affect the performance of the medium.n2. Adjust the pH to 0.2-0.3 pH units below the desired pH using 1N HCl or 1N NaOH since the pH tends to rise duringnfiltration.n3. Make up the final volume to 1000ml with tissue culture grade water.n4. Sterilize the medium immediately by filtering through a sterile membrane filter wit