汉黄芩素作用机制 - Medchemexpress - MCE中国

1、Product Data SheetWogoninCat. No.: HY-N0400CAS No.: 632-85-9分式: CHO分量: 284.26作靶点: CDK; Wnt; Autophagy; Apoptosis作通路: Cell Cycle/DNA Damage; Stem Cell/Wnt; Autophagy; Apoptosis储存式: 4C, protect from light* In solvent : -80C, 6 months; -20C, 1 month (protect from light)溶解性数据体外实验 DMSO : 48 mg/mL (168.86

2、 mM)* means soluble, but saturation unknown.SolventMass1 mg 5 mg 10 mgConcentration制备储备液1 mM 3.5179 mL 17.5895 mL 35.1791 mL5 mM 0.7036 mL 3.5179 mL 7.0358 mL10 mM 0.3518 mL 1.7590 mL 3.5179 mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存式和期限：-80C, 6 months; -20C, 1 month (protect fro

3、m light)。-80C 储存时，请在 6 个内使，-20C 储存时，请在 1 个内使。BIOLOGICAL ACTIVITY物活性 Wogonin种天然黄酮类物质，能够抑制 CDK8 和 Wnt 的活性，具有抗炎、抗肿瘤等功效。IC & Target CDK8 Wnt体外研究 Wogonin (0-200 M) exhibits a dose- and time- dependent reduces in cell viability of caco-2, SW1116 and HCT116cells. Wogonin (10-40 M) induces G1 phase arrest i

4、n HCT-116 cells. Wogonin also supresses Wnt signaling pathwayin HCT116 cells. Wogonin interfers in the activity of transcription factor TCF/Lef family. Moreover, Wogonin inhibits -catenin-mediated transcription through suppressing the activity of CDK81. Wogonin shows cytotoxic andantiproliferative e

5、ffects on HeLa cells. Wogonin (90 M) induces cell cycle arrest at G0-G1 phase, and suppresses thelevels of cyclin D1 and Cdk4 markedly in HeLa cells2. Wogonin (1.25, 2.5, 5, 10, 20 g/ml) suppresses EtOH-inducedinflammatory response in RAW264.7 cells3.体内研究Wogonin (30, 60 mg/kg) reduces tumor growth o

6、f HCT116 cells in a xenograft model1. Wogonin (25, 50, 100 mg/kg)Page 1 of 2 www.MedChemEprotects against liver injury and pathological characteristics of ALD in mice. Wogonin activates PPAR- expression inmice with ALD and EtOH induced RAW264.7 cells3.PROTOCOLCell Assay 1 HCT116 cells are planted on

7、 a 96-well plate (1 105 cells per well). Different concentrations of wogonin are addedand incubated for 24 h. Subsequently, 20 L of MTT solution (5 mg/mL) is transferred to each well and the plates areincubated for 4 h at 37C and 5% CO2. The supernatant is aspirated off and 100 L DMSO is added to di

8、ssolve theformazan crystal. The mixture is shaken and measured at 570 nm using a universal microplate reader.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Animal C57BL/6 mice, male, 6-8 weeks old, weighing 18-22 g mice are housed at comfortable enviro

9、nment and areAdministration 3 acclimatized for 3 days before the experiment. A total of 48 mice are randomLy divided into six groups of 8 animals,respectively control diet (CD)-fed mice, EtOH-fed mice, wogonin-treated mice at the dose of 25, 50, 100 mg/kg/dayand the positive (dexamethasone, 1 mg/kg/

10、day)-treated mice. Modeling process has a total of 16 days including aliquid diet adaptation period for 3 days and modeling for 13 days. The EtOH-fed mice are fed containing 5% v/vethanol liquid diets adding certain vitamin and choline for 16 days, and mice are gavaged with a single binge ethanoladm

11、inistration (5 g/kg, body weight, 20% ethanol) at last day. At the same time, the wogonin-treated mice and thepositive-treated mice are not only plus the ethanol administration, but also plus the medicines by gavage daily,whereas the CD-fed mice are fed with control liquid diets and gavaged with iso

12、caloric maltose-dextrin at last day. Alldiets are prepared fresh daily. 9 h after the last gavage alcohol, mice are sacrificed under anaesthesia, the liver tissuesand blood are collected for further analysis.MCE has not independently confirmed the accuracy of these methods. They are for reference on

13、ly.户使本产品发表的科研献 Pharmacol Res. 2020 Mar. Exp Cell Res. 2020 May 3:112054. Eur J Pharmacol. 2019 Apr 5;848:121-130 Xenobiotica. 2020 Feb 12:1-7.See more customer validations on HYPERLINK www.MedChemE www.MedChemEREFERENCES1. He L, et al. Wogonin induced G1 cell cycle arrest by regulating Wnt/-catenin

14、signaling pathway and inactivating CDK8 in human colorectal cancercarcinoma cells. Toxicology. 2013 Oct 4;312:36-47.2. Yang L, et al. Wogonin induces G1 phase arrest through inhibiting Cdk4 and cyclin D1 concomitant with an elevation in p21Cip1 in human cervicalcarcinoma HeLa cells. Biochem Cell Biol. 2009 Dec;87(6):933-42.3. Li HD, et al. Wogonin attenuates inflammation by activating PPAR- in alcoholic liver disease. Int Immunopharmacol. 2017 Sep;50:95-106.McePdfHeightPage 2 of 3 www