iCRT-14 _Wnt 抑制剂 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEiCRT 14Cat. No.: HY-16665CAS No.: 677331-12-3分式: CHNOS分量: 375.44作靶点: Wnt作通路: Stem Cell/Wnt储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 29 mg/mL (77.24 mM)* means soluble, but saturation

2、unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 2.6635 mL 13.3177 mL 26.6354 mL5 mM 0.5327 mL 2.6635 mL 5.3271 mL10 mM 0.2664 mL 1.3318 mL 2.6635 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL ACTIVITY物活性 iCRT 14种 -连环蛋应答转录（CRT）抑制剂，能够抑制 Wnt 信号通路，IC50 值为 40.3 nM。IC50 & Target IC50

3、: 40.3 nM (Wnt responsive STF16 luciferase) 1体外研究iCRT14 can interfere with TCF binding to DNA in addition to its ability to influence TCF-cat interaction 1.iCRT14 (10, 25, 50 M) effectively inhibits cell proliferation in BT-549 cells in a dose- and time-dependent1/2 Master of Small Molecules 您边的抑制剂师

4、www.MedChemEmanner, but still less potent than iCRT3 2.体内研究 iCRT14 (50 mg/kg, i.p.) markedly decreases CycD1, proliferation of the tumors in HCT116 xenografts 1.PROTOCOLCell Assay 2 Cells are seeded at 20,000 cells/well into 96-well plates. After overnight incubation, cells are treated withDMSO or e

5、ach Wnt inhibitor (iCRT-3, 75 M; iCRT-5, 200 M; iCRT-14, 50 M; IWP-4, 5 M and XAV-939,10 M) for 48 hours. Cell viability is determined using the Cell Titer-Glo luminescent cell viability assay kit.Luminescence is measured using FLUOstar microplate reader. All treatments are performed in triplicate,

6、andeach experiment is repeated three times.MCE has not independently confirmed the accuracy of these methods. They are for reference only.户使本产品发表的科研献 Cell Death Dis. 2018 Apr 1;9(4):433.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Gonsalves FC, et al. An RNAi-based chemical

7、genetic screen identifies three small-molecule inhibitors of the Wnt/wingless signalingpathway. Proc Natl Acad Sci USA. 2011 Apr 12;108(15):5954-63.2. Bilir B, et al. Wnt signaling blockage inhibits cell proliferation and migration, and induces apoptosis in triple-negative breast cancer cells.J Transl Med. 2013 Nov 4;11:280.McePdfHeightCaution: Product has not been fully validated for medical applications.For research