Vanillin-p-Vanillin-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEVanillinCat. No.: HY-N0098CAS No.: 121-33-5Synonyms: p-Vanillin; m-Methoxy-p-hydroxybenzaldehyde; p-Hydroxy-m-methoxybenzaldehyde分式: CHO分量: 152.15作靶点: Endogenous Metabolite作通路: Metabolic Enzyme/Protease储存式: 4C, stored under nitr

2、ogen* In solvent : -80C, 6 months; -20C, 1 month (stored undernitrogen)溶解性数据体外实验 DMSO : 100 mg/mL (657.25 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 6.5725 mL 32.8623 mL 65.7246 mL5 mM 1.3145 mL 6.5725 mL 13.1449 mL10 mM 0.6572 mL 3.2862 mL 6.5725 m

3、L请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。体内实验请根据您的实验动物和给药式选择适当的溶解案，配制前请先配制澄清的储备液，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的作液，建议您现现配，当天使；澄清的储备液可以根据储存条件，适当保存；以下溶剂前的百分 指该溶剂在您配制终溶液中的体积占)：1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (16.43 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 9

4、0% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (16.43 mM); Clear solution1/3 Master of Small Molecules 您边的抑制剂师www.MedChemE3. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (16.43 mM); Clear solutionBIOLOGICAL ACTIVITY物活性 Vanillin从草中提取的单个分，也 在，品和药物中泛使的味。IC50 & Target Human Endogenous Metabolit

5、e体外研究 Vanillin recovers UVA-induced reduction of proliferation in a dose dependent manner. Vanillin has noapoptotic effects at the tested concentrations. In addition, the reduced expression levels of OCT4, NANOG,and SOX2 caused by UVA irradiation are allincreased by Vanillin treatment, suggesting th

6、at Vanillinattenuates the effects of UVA irradiation on hAMSCs. Based on a luciferase reporter assay, Vanillinincreases the reduced activity of HRE-luciferase reporter caused by UVA irradiation. In addition, treatmentwith Vanillin attenuates the reduced expression of HIF-1 caused by UVA irradiation.

7、 The results reveal thattreatment of hAMSCs with Vanillin results in significant decrease in the production of PGE2 and cAMP whencompare to UVA-irradiated controls 1.体内研究 Following antidepressant treatment for 4 weeks, immobility time in the stress+Vanillin and stress+fluoxetinegroups is significant

8、ly decreased F(4,42)=34.73, P 2.PROTOCOLKinase Assay 1 hAMSCs are irradiated with the indicated doses of UVA and then incubated with the indicated concentrationsof Vanillin for three days under serum-free conditions. After the incubation, the concentrations of PGE2 orcAMP in the culture supernatant

9、are measured using ELISA kits. Culture supernatants are added into 96 wellplates. Alkaline phosphatase conjugated PGE2 or cAMP and antibodies to PGE2 or cAMP are added tosample wells and incubated at room temperature for 2 h. The sample wells are then washed with PBS and p-nitrophenyl phosphate (pNp

10、p) substrate solution is added. Finally, the samples are incubated at roomtemperature for 1 h and their absorbance values are read according to the manufacturers instructions 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Cell Assay 1 hAMSCs are irra

11、diated with the indicated doses of UVA and then incubated with 1 to 100 M of Vanillin forthree days under serum-free conditions (in DMEM devoid of serum, at 37C with 5% CO2). After three days,cell proliferation is measured using BrdU incorporation assay 1.MCE has not independently confirmed the accu

12、racy of these methods. They are for reference only.Animal Male Sprague-Dawley rats (200 to 250 g) are used in this study. The animals are divided into three groupsAdministration 2 with 8 to 10 rats per group: the stress+fluoxetine group; the stress+Vanillin aromatherapy group and thestress (untreate

13、d) group. For the stress+fluoxetine group, the animals are administered a daily oral dose (10mg/kg/d, diluted in distilled water) of the SSRI fluoxetine each morning. For the stress+Vanillin group and thebulbectomy+Vanillin group, Vanillin is administrated in a Plexiglas cylinder 50 cm tall and 35 c

14、m diameter2/3 Master of Small Molecules 您边的抑制剂师www.MedChemEwith two layers separated by a porous Plexiglas board. The rat still in its cage is gently placed on the upperlayer, and 5 mL of 600 mg/L Vanillin (in distilled water) sprayed on to the floor of the lower layer. Rats in thestress and the con

15、trol groups receive similar handling to the stress+Vanillin group, but without any odoradministrated 2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Lee SY, et al. Vanillin attenuates negative effects of ultraviolet A on the stemness of human adipose tissue-derived mesenchymal stemcells. Food Chem Toxicol. 2016 Oct;96:62-9.2. Xu J, et al. Vanillin-induced amelioration of depression-like behaviors in rats by modulating monoamine neurotransmitters in the brain.Psychi