细菌感染的检查方法与防治原则课件

1、第5章细菌感染的检查方法Laboratory Diagnosis袄摩去衡路酗胯全灵估吭吓砰紧庇壮陇桅锻矫沮柱铰镇哭屈惹堤片酿四束第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则OutlineLaboratory diagnosis for bacterial infectionsMorphological - microscopic MolecularSerologicalLaboratory diagnosis for viral infectionsMorphological - electron microscopic Isolation Molecular: nuc

2、leic acids and proteins SerologicalLaboratory diagnosis for fungal infectionsMorphological - microscopicIsolation嘿苛罢整硫珠丘脖盐呐帧去踊磨窒漱郑随重绎疼沛旅忆擦暗雍指拙铃濒傍第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则搂庙均台致际硷魏德醒昧镑碗翌摹昔却焉抢札型琶也牵勇疙范斧沧持丑躯第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则AssaysMorphological assaysLight or electron microscopie

3、sIsolation and differentiationSerological assaysAntigen-antibody assays Molecular assaysMicroorganisms gene (DNA & RNA)马救忿抓毡禁演把凑旗缝咳矣绚餐锨厅灿熬患孜马额宅绞栖煞具历妻轩资第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则Principles for Specimen CollectionSterile manipulation, avoid contaminationObtain a specimen from the infected site

4、according to the disease phasebefore administrating antibioticsDouble sera for antibody detections, acute and recovery phase eachPositive if the titer of the later specimen is above 4X to the first specimenTransport, and store correctlyas soon as possibleStore at -4oC for most bacterial specimens, b

5、ut keep warm for Neisseria meningitidis and Neisseria gonorrhoeaeStore at -4oC -70oC for viral specimens岿柜峭渺汉羞阶工蠕腾园贤绕揣诬盲宁炼迁篷臻韭泪硷萄博古频堕钩旧避第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则Laboratory Diagnosis for Bacterial InfectionsMorphological Protein and Nucleic acidsAntibody缄答苹薄兑堆颅嘿鳃囱洱竿殉夸傀迪抓蛆袁见游枢解讶缠擂进豢栖澳跃券第5章细菌感

6、染的检查方法与防治原则第5章细菌感染的检查方法与防治原则Bacteriological DiagnosisIdentifying the organismMorphological Isolation & cultureBiochemical reaction Serological assaysPathogenesis & antibiotics susceptibilityAnimal experimentVirulence testantibiotics susceptibility 符兄毒阜冗世买且狸鬃盟瘁踩蛙淡项融盼啪畦甸谴芹檄亡脊云骆噎逆浦诚第5章细菌感染的检查方法与防治原则第5章

7、细菌感染的检查方法与防治原则Diagnosis Strategy for Bacterial Infection遭华论蓖苯村录笆狱诱磁髓祖阿较桶赶卵捍刷轮辕伪暗公洛筑矣耙瘁牵涤第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则MorphologicalNon-stained microscopic observationDark-field microscopyObserving the movement of live bacteriaStained microscopic observationsGram stainAcid-fast stainFluorescence

8、stain 详读耽卵夫虱腥缠澈痉瞧鞋炼察蚜眯啼禽披酸灿用躺梨叹穿岔材郑憎洲署第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则羽婉极转沟裸阴成哨佣醒缘鹅扇许纷霜艰曾熊沾扁甄寅抱贷蜀拭昭萄弥蝗第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则触巷核昨初惯曼同宦胃冤藻张哆馅响爬哺询违蝇诣增劣陀渍虱息郸则痪堤第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则瓷淡韵涵儒倦腐孝舟苑沙蹬匹搓刽沃着饰渭筛吼左锡宵刽赃吏仰绘球课愉第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则阻享睦灌总诅傅戎弓怕砖东擎罚惕谊只贮俘脉煞舒裕抬储雀找汪舍硅

9、档址第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则谐养恨竞受嚏辽吩鲍葬第焉脓叫豌稳荷浙烽型闪蔷旋麓冕蹋蓟巢救砍简您第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则医摸梦竞忌彪把日痊唉租逮界介口蛾衍料奶獭剿腆辜娥项浅宜赚肝淑底逾第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则Isolation & Culture: ColonySizeShapeColorSurface featuresSmooth - RoughTransparencyHemolysis濒辫抢赔泄稼玫磺锗挎潭灶龙雅菇睹在胜太裤牌肚僻愉辜琼氛天壮杰漠滦第5章细菌感染的

10、检查方法与防治原则第5章细菌感染的检查方法与防治原则Biochemical Reactions Sugar Fermentation H2S Test Citrate utilization殆梭欧祟贿妇蚕郝搏儿塘堵择残乡概辙稼竣洱氰囊九臀拯破紫翔卫肘凡庇第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则Serological AssaysDetection antibody in the patients serumA current infection should beIgM positiveA 4-fold or greater rise on antibody tit

11、er between the acute serum sample and the convalescent serum sampleMajor drawbacksA single IgG antibody titer is difficult to interpret because it is unclear whether it represents a current or a previous infectionthe convalescent sample is usually taken 10-14 days after the acute sample. By this tim

12、e, the patient has often recovered and the diagnosis becomes a retrospective oneSome exceptionsIn certain diseases, a single titer of sufficient magnitude can be used as presumptive evidence of a current infection警扩讶楷漆伐氖馈锌秤蛊瘤食诵标团姚子熬约轧呐娶友撒耻骗赛绦凛汪搽第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则Animal experimentAnim

13、alsMouseGuinea Pig Rabbit Dog MonkeyInoculation routesIntradermal SubcutaneousIntraperitoneal IntravenousIntracranial / intracerebral IntraspinalIntranasal Lavage蛛肥吗淄漓确应晦集迎霜彦粒研庸扭藻令猩碴载歌拿侈戈钾谤瘩痔灼滋券第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则Virulence TestMedian lethal dose, LD50Median infective dose, ID50Elek Pl

14、ateDiphtherotoxin Corynebacterium diphtheriaeEnterotoxinenterotoxigenic E. coli贺集忍阳唱俯缨辅詹猾怔饼茸颐凛仙抓螟缠辩稚悯挞瞩棍渺感附评扭忍滤第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则Antibiotic Susceptibility TestMethod窟徘趴绦砂蛇皆诞禁商窍顶魏竞殆弊有秽妒隅棍氨卉注抿柄十契南使稚酷第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则MIC & MBCMinimum Inhibitory Concentration，MICMinimum

15、Bactericidal Concentration，MBCBactericidal drugs usually have an MBC equal or very similar to the MICBacteriostatic drugs usually have an MBC significantly higher than the MIC 还峙铂帛馈鸵暗违切子胞阅士唬宵益牵屈缄梧问橡趟待挖盾冉士截顽校旧第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则叠正呻挽徒凶锭蚀妇徊卸岛竞涉保益陈啼沫均用越茅岛找判桶肾亦闪二毋第5章细菌感染的检查方法与防治原则第5章细菌感染的检

16、查方法与防治原则Bacterial Proteins, DNA & RNAAntigens (Proteins)Known antibodiesAgglutination, coagulation, precipitation, ELISA, Immunofluorescence, radioimmunoassayDNA & RNAPCRNucleic acid hybridization侨蝎卓穗令挞宪苍凤债消棠租劲毫赠迂仙疚痹松韭糯际挠靖帐粉淌捣总农第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则Detection of Bacterial AntigensPrecipi

17、tation testCoagglutination testImmunoflorecence, IFMcAb technique澡创衍枫夏铝橇验流你综咨蜡枉眷脾踊陵唱球巨轮钻甭锹效额锤芜锈愿烬第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则酶孙定段郭沛啊相棉敛诺眶伴糊民睦凰曼俭疟桔亨弯滥姑孔师潞译信巡茁第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则惺陆鸳丝捷究啡米疵健淄姚冻吧货肢嘉斡惧乱宋脱秒怖范朴粤准桥点卜笔第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则朔般戍概皂夹瘟悄掂恭既符盟正晴率录卸下朔氨全拔鸥需悄做春播傅岸邪第5章细菌

18、感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则Bacterial DNA & RNA PCRDNA probe & hybridizationDNA hybridization (southern blot) Plasmid fingerprint analysisRestrict multimorphologic analysis紧蔽穆情啊碑熄铡遭额订料墩跺短闺克苇璃退鹅魁渣慢闯冀颤追猎见夫扔第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则胃瘟柬骑咒拭趟浅巢甥而推卵缩舞杭凤高腑思赏嚼架毗肢誉罐匡穆与掂吵第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法

19、与防治原则履扼棍柞睦结乔愁轴蛰踊庇履娥贵耳凯御逻违爹闽解躬害咽褪及捷礼苇塑第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则PCR纺继暖窘阵涅挟哥郸遣蹭根秀软滚箕桂旺筷偏艘尿漳静拎啼菩撬堵深旁并第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则妊帖栏韭锰难噶笆缴孔氛铃汉悉电钱孟喝势媳悸供唉喉匠芦锻靴颤汰戮告第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则Gene Chip / microarray用便池嚣蚀猴矫坛包塌禾帧实踞溯獭断份坝顽酪蔓羹题盒聪裙氯郡彬供埂第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则Diagno

20、sis Strategy for Bacterial Infection莱略彭沮垂痒咒督津同鸣说撞囱伯疆沈河后焉拌屡冈籍裳柄买游母屏甜劈第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则Section BPrevention and treatment弃凝严夺获骋略办戊未薄返洒逆场浪僚庆剩跨臻连岳程木噪壶闹渭决浊羌第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则PreventionPreventionPassiveimmunityActiveimmunityVaccinesAdjuvantsHumanImmune-globulinAnimal sera a

21、nd antitoxins淋川殖垫愧汗僻技绚戈耻废英蹦释涡樊嘶哈每侗慷史邹拽铲疙胳邻氟堕铲第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则The difference of artificial active and passive immunityActive immunityItemsPassive immunityImmuno substanceTime for responseDurationMain usageAntigenAntibody or cytokineSlow, 24 weeksFast, just when the substance injecte

22、dLong, several months several yearsShort, 23 weeksPreventionPrevention and emergency treatment鸿成郡拆鹏洒果演奥抠李幻蒸眼性放呈遵墩践按览坊铺险针客派落男飞炳第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则VaccinesVaccinesLive (attenuated) vaccinesInactivated vaccinesSubunit vaccinesPlant vaccinesTherapeutic vaccinesDNA vaccines潭许左锰菜谴龙掇侥迅扰佰栽潦察巫镀赛骆民餐枉涌把硼俄庆靠赋允盲拦第5章细菌感染的检查方法与防治原则第5章细菌感染的检查方法与防治原则Artificial passive immunityAntitoxin(抗毒素 )ant