ML348-GNF-Pf-1127-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEML348Cat. No.: HY-100736CAS No.: 899713-86-1Synonyms: GNF-Pf-1127分式: CHClFNO分量: 415.79作靶点: Phospholipase作通路: Metabolic Enzyme/Protease储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 25 mg/m

2、L (60.13 mM; Need ultrasonic)Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 2.4051 mL 12.0253 mL 24.0506 mL5 mM 0.4810 mL 2.4051 mL 4.8101 mL10 mM 0.2405 mL 1.2025 mL 2.4051 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。体内实验 请根据您的实验动物和给药式选择适当的溶解案，配制前请先配制澄清的储备液，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的作液，建议您现现配

3、，当天使；澄清的储备液可以根据储存条件，适当保存；以下溶剂前的百分 指该溶剂在您配制终溶液中的体积占)：1. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (6.01 mM); Clear solutionBIOLOGICAL ACTIVITY1/2 Master of Small Molecules 您边的抑制剂师www.MedChemE物活性 ML348个选择性的和可逆的 LYPLA1 抑制剂, IC50 值为 210 nM 1。IC50 & Target IC 50: 210 nM (LYPLA1)体外研究 ML348 (5

4、M; 3 hours ) selectively inhibits LYPLA1 in cultured cells 1.Cell Viability Assay 1Cell Line: HEK293T cells, murine T-cellsConcentration: 5 MIncubation Time: 3 hoursResult: Show potent, selective inhibition of LYPLA1.体内研究 ML348 (50 mg/kg, i.p., 3 hours) shows potent and selective activity in vivo, i

5、nhibits LYPLA1 enzymes in heart,kidney and lung in mice 1.Animal Model: C57BL6 male mice (3-4 months) 1Dosage: 50 mg/kgAdministration: Intraperitoneal injection; 3 hoursResult: Potent and selective inhibited LYPLA1 enzymes in heart, kidney and lung in vivo.户使本产品发表的科研献 Nat Biomed Eng. 2019 Mar.See mo

6、re customer validations on HYPERLINK / www.MedChemEREFERENCES1. Adibekian A et al. Characterization of a Selective, Reversible Inhibitor of Lysophospholipase 1 (LYPLA1). National Center forBiotechnology Information (US); 2010- 2013 Apr 08.McePdfHeightCaution: Product has not been fully validated for medical applications.For research use only.Tel: 400-8