常用蛋白质分子量标准参照物(精)

1、十、常用蛋白质分子量标准参照物高分子量标准参照中分子量标准参物低分子量标准参照蛋白质Mr蛋白质Mr蛋白质Mr肌球蛋白212000磷酸化酶B97400碳酸酐酶310000-半乳糖苷酶116000牛血清清蛋白66200人豆胰蛋白酶制剂21500磷酸化酶B97400谷氨酸脱氢酶55000马心肌球蛋白16900牛血清清蛋白66200卵清蛋白42700溶菌酶14400过氧化氢酶57000醛缩酶40000肌球蛋白（F1）8100醛缩酶40000碳酸酐酶31000肌球蛋白（F2）6200人豆胰蛋白酶抑制剂21500肌球蛋白（F3）2500溶菌酶14400十一、层析法常用数据表及性质（一）离子交换纤维素目前常

2、用的离子交换纤维素列于下表DEAE-纤维素形状长度（微米）交换当量（毫克当量/克）蛋白吸附容量（毫克/克）床体积（毫升/克）胰岛素(pH8.5)牛血清清蛋白（pH8.5）pH6.0pH7.5DE-22改良纤维性*124001.00.17504507.77.7DE-23同上（除细粒）184001.00.17504508.39.1DE-32微粒性（干粉）24631.00.18506606.06.3DE-52同上（溶胀）24631.00.18506606.06.3CM-纤维素溶菌酶7S-y球蛋白pH5.0pH7.5(pH5.0)(pH5.0)CM-22改良纤维性124000.60.066001507

3、.77.7CM-23同上（除细粒）184000.60.066001509.19.1CM-32微粒性（干粉）24631.00.11,2604006.86.7CM-52同卜（溶胀）24631.00.11,2604006.86.7*:英国Whatman厂的型号，原来有旧型号如DE-1,为长纤维性，长度1000微米。还有DE-11,纤维性，50一250微米，对牛血清清蛋白的吸附容量仅为130毫克/克。（二）聚丙烯酰胺凝胶的技术数据型号排阻的下限（Mr）分级分离的范围（Mr）膨胀后的床体积（mL/g干凝胶）膨胀所需最少时间（室温，小时）Bio-gel-P-2160020020003.824Bio-gel

4、-P-4360050040005.824Bio-gel-P-6460010005000&824Bio-gel-PTO1000050001700012.424Bio-gel-P-3030000200005000014.91012Bio-gel-P-6060000300007000019.01012Bio-gel-P-1001000004000010000019.024Bio-gel-P-1501500005000015000024.024Bio-gel-P-2002000008000030000034.048Bio-gel-P-30030000010000040000040.048注：上述各种型

5、号的凝胶都是亲水性的多孔颗粒，在水和缓冲溶液中很容易膨胀，生产厂为Bio-RadLaboratories,Rich-mond,California,U.S.A.三）琼脂糖凝胶的技术数据（琼脂糖是琼脂内非离子型的组分，r它在0r,pH49范围内是稳定的。名称、型号凝胶内琼脂糖百分含量（W/W）排阻的下限（Mr）分级分离的范围（Mr）生产厂商Sagavac10102.5)1051x1042.5x05SeravacLaboratories,Sagavac887x1052.5x047x105Maidenhead,EnglandSagavac662x1065x1042x106Sagavac4415X06

6、2x10515x106Sagavac22150 x1065x10515x107Bio-GelA-0.5M100.5X051x1042.5x06Bio-RadLaboratories,Bio-GelA-1.5M81.5X061x1041.5x06California,U.S.A.Bio-GelA-5M65x1061x1045x106Bio-GelA-15M415x1054x10415x106Bio-GelA-50M250X1061x10550 x106Bio-GelA-150M1150 x1061x106150 x106（四）凝胶过滤层析介质的技术数据凝胶过滤介质名称分离范围颗粒大小（um）特性

7、/应用pH稳定性工作耐压(Mpa)最快流速（cm/h）Superdex30100002444肽类、寡糖、小蛋白等3120.3100Superdex753000700002444重组蛋白、细胞色素3120.3100Superdex2000100006000002444单抗、大蛋白3120.3100Superose650005x1062040蛋白、肽类、多糖、核酸3120.430Superose1210003000002040蛋白、肽类、寡糖、多糖3120.730SephacrylS-100HR10001000002575肽类、小蛋白3110.22039SephacrylS-200HR500025

8、00002575蛋白，如清蛋白3110.22039SephacrylS-300HR100001.51062575蛋白、抗体3110.22039Sephacryl200008x1062575多糖、具延伸结构的大分3110.22039S-400HR子如蛋白多糖、脂质体Sepharose6FastFlow10000406平均90巨大分子2120.1300Sepharose4FastFlow600002006平均90巨大分子如重组乙型肝炎表面抗原2120.1250Sepharose2B70000400660200蛋白、大分子复合物、病毒、不对称分子如核酸和多糖（蛋白多糖）490.00410Sephar

9、ose4B60000200645165蛋白、多糖490.00811.5Sepharose6B1000040645165蛋白、多糖0.0214Sepharose70000400660200蛋白、大分子复合物、病490.00515CL-2B毒、不对称分子如核酸和多糖（蛋白多糖）SepharoseCL-4B60000200545165蛋白、多糖3130.01226SepharoseCL-6B1000040645165蛋白、多糖3130.0230凝胶过滤介质名称分离范围颗粒大小(“m)特性/应用pH稳定性工作干凝胶溶胀体积(mL/g)溶胀最少平衡时间(h)最快流速(cm/h)室温沸水SephadexG

10、-10700干粉40120213233125SephadexG-15105干粉40120中等蛋白质分离210152048547SephadexG-100Superfine4000lx105干粉1040中等蛋白质分离210152048511SephadexG-15050003x105干粉40120稍大蛋白质分离210203072521SephadexG-150Superfine50001.5105干粉1040稍大蛋白质分离21018227255.6SephadexG-20050006x105干粉40120较大蛋白质分离210304072511SephadexG-200Superfine50002

11、.505干粉1040较大蛋白质分离21020257252.8SephadexLH20100干粉特别为使用有机溶剂而设计。适合分离脂类、胆固醇、脂肪酸、激素、(嗜脂性)400025106维牛素及其他小牛物分f子。此分、离范围指以酒勿精为溶剂1的分离(五)离子交换层析介质的技术数据离子交换介质名称最高载量颗粒大小(“m)特性/应用pH稳定性工作耐压(MPa)最快流速(cm/h)SOURCE15Q25mg蛋白1521241800SOURCE15S25mg蛋白1521241800QSepharoseHP70mgBSA24442120.3150SPSepharoseHP55mg核糖核酸酶24443120

12、.3150QSepharoseFF120mgHSA45一1652120.2400SPSepharoseFF75mgBSA45一1654130.2400DEAESepharoseFFllOmgHSA45一165290.2300CMSepharoseFF50mg核糖核酸酶1003006130.2300QSepharoseBigBeads1003002120.312001800SPSepharoseBigBeads60mgBSA干粉401204120.312001800离子交换介质名称最高+卜载量颗粒大小(“m)特性/应用pH稳定性工作耐压(MPa)最快流速(cm/h)QAESephadexA-25

13、1.2mg甲状腺球蛋白80mgHSA干粉40120纯化低分子量蛋白、多肽、核苷以及巨大分子(Mr200000)，在工业传统应用上具有重要作用2100.11475QAESephadexA-501.2mg甲状腺球蛋白80mgHSA干粉40120批量生产和预处理用，分离中等大小的生物分子(30一200000)2110.0145SPSephadexC-25l.lmgIgG70mg牛羰合血红蛋白230mg核糖核酸酶干粉40120纯化低分子量蛋白、多肽、核苷以及巨大分子(Mr200000)，在工业传统应用上具有重要作用2100.13475SPSephadexC-508mgIgGllOmg牛羰合血红蛋白干粉

14、40120批量生产和预处理用，分离中等大小的生物分子(30200000)2100.0145DEAESephadexA-251mg甲状腺球蛋白30mgHAS140mga-乳清蛋白干粉40120纯化低分子量蛋白、多肽、核苷以及巨大分子(Mr200000),在工业传统应用上具有重要作用2一90.11475DEAESephadexA-502mg甲状腺球蛋白llOmgHSA干粉40120批量生产和预处理用，分离中等大小的生物分子(Mr200000),在工作传统应用上具有重要作用2一90.1145CMSephadexC-251.6mgIgG70mg牛羰合血红蛋白190mg核糖核酸酶干粉40120纯化低分子

15、量蛋白、多肽、核苷以及巨大分子(Mr200000),在工业传统应用上具有重要作用6130.13475CMSephadexC-507mgIgG140mg牛羰合血红蛋白120mg核糖核酸酶干粉40120批量生产和预处理用，分离中等大小的生物分子(30200000)6100.0145十二、常用缓冲溶液的配制方法1.甘氨酸-盐酸缓冲液（005mol/L）X毫升0.2mol/L甘氨酸+Y毫升0.2mol/LHCI,再加水稀释至200毫升pHXYpHXY2.05044.03.05011.42.45032.43.250&22.65024.23.4506.42.85016.83.6505.0甘氨酸分子量=75

16、.070.2mol/L甘氨酸溶液含15.01克/升。2.邻苯二甲酸-盐酸缓冲液（0.05mol/LX毫升0.2mol/L邻苯二甲弓酸氢钾+0.2mol/LHCl,再加水稀释到20毫升pH(20C)XYpH(20C)XY2.254.0703.251.4702.453.9603.450.9902.653.2953.650.5972.852.6423.850.2633.052.022邻苯二甲酸氢钾分子量=204.230.2mol/L邻苯二甲酸氢溶液含40.85克/升3.磷酸氢二钠-柠檬酸缓冲液pH0.2nol/LNa2HPO4(毫升)0.1nol/L柠檬酸（毫升）pH0.2nol/LNa2HPO4(

17、毫升)0.1nol/L柠檬酸（毫升）2.20.4010.605.210.729.282.41.2418.765.411.158.852.62.1817.825.611.60&402.83.1716.835.812.097.913.04.1115.896.012.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.473.534.28.2811.727.217.392.614.48.8211.187.418.1

18、71.834.69.3510.657.618.731.274.89.8610.147.819.150.855.010.309.70&019.450.55Na2HPO4分子量=14.980.2mol/L溶液为28.40克/升。Na2HP04-2H2O分子量=178.050.2mol/L溶液含35.01克/升。C4H2O7H2O分子量=210.140.1mol/L溶液为21.01克/升。4.柠檬酸-氢氧化钠-盐酸缓冲液pH钠离子浓度（mol/L）柠檬酸（克）C斗o7H2o氢氧化钠克）NaOH97%盐酸（毫升）HCl（浓）最终体积（升）2.20.2021084160103.10.2021083116

19、103.30.2021083106104.30.202108345105.30.3524514468105.80.45285186105106.50.3826615612610使用时可以每升中加入1克克酚，若最后pH值有变化，再用少量50%氢氧化钠溶液或浓盐酸调节，冰箱保存。5.柠檬酸-柠檬酸钠缓冲液(0.1mol/LpH0.1mol/L柠檬酸（毫升）0.1mol/L柠檬酸钠（毫升）pH0.1mol/L柠檬酸（毫升）0.1mol/L柠檬酸钠（毫升）3.018.61.45.0&211.83.217.22.85.27.312.73.416.04.05.46.413.63.614.95.15.65.

20、514.53.814.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.4&66.42.018.04.610.39.76.61.418.64.89.210.8柠檬酸C6H8O7H2O:分子量210.140.1mol/L溶液为21.01克/升。柠檬酸钠Na3C6H5072H20:分子量294.120.1mol/L溶液为29.41克滝升。6乙酸-乙酸钠缓冲液（0.2mol/LpH（18C）0.2mol/LNaAc毫升）0.3mol/LHAc毫升）pH（18C）0.2mol/LNaAc毫升）0.3mol/LHAc毫升）2.60.7

21、59.254.85.904.103.81.208.805.07.003.004.01.808.205.27.902.104.22.657.355.48.601.404.43.706.305.69.100.904.64.905.105.89.400.60Na2AcH20分子量=136.090.2mol/L溶液为27.22克/升。7磷酸盐缓冲液（1）磷酸氢二钠-磷酸二氢钠缓冲液（0.2）pH0.2mol/LNa2HPO4毫升）0.3mol/LNaH2PO4毫升）pH0.2mol/LNa2HPO4毫升）0.3mol/LNaH2PO4毫升）5.8&092.07.061.039.05.910.090.0

22、7.167.033.06.012.387.77.272.028.06.115.085.07.377.023.06.218.581.57.481.019.06.322.577.57.584.016.06.426.573.57.687.013.06.531.568.57.789.510.56.637.562.57.891.5&56.743.556.57.993.07.06.849.551.0&094.75.36.955.045.0Na2HPO4H20分子量=178.050.2mol/L溶液为85.61克/升。Na2HP04H20分子量=358.220.2mol/L溶液为71.64克/升。Na2HP

23、O4H20分子量=156.030.2mol/L溶液为31.21克/升。(2)磷酸氢二钠-磷酸二氢钾缓冲液(1/15mol/L)Hpmol/L/15Na2HPO4（毫升）mol/L/15KH2PO4（毫升）Hpmol/L/15NaHP04（毫升）mol/L/15KH2P04毫升）40.109.9077.003.00.920.509.50.178.002.0051.009.0079.001.00.292.008.00.389.500.5053.007.0079.750.25.914.006.00.739.900.1065.005.00810.000.246.004.00.0468.47.3468.

24、64.6768.81.186.98Na2HPO4H20分子量=178.051/15M溶液为11.876克/升。KH2PO4分子量=136.091/15M溶液为9.078克/升。&磷酸二氢钾-氢氧化钠缓冲液(0.05M)X毫升0.2MK.PO4+Y毫升0.2NNaOH加水稀释至29毫升I24I|IpH（20C）24X毫升)Y(毫升)pH（20C）X毫升)Y(毫升)5.850.3727.052.9636.050.5707.253.5006.250.8607.453.9506.451.2607.654.2806.651.7807.854.5206.852.365&054.6809巴比妥钠-盐酸缓冲液

25、（18乜）pH0.04M巴比妥钠溶液0.2V盐酸pH0.04M巴比妥钠溶液（毫升）0.2N盐酸（毫升）6.810018.4&41005.217.010017.8&61003.827.210016.7&81002.527.410015.39.01001.657.610013.49.21001.137.810011.479.41000.70&01009.399.61000.35&21007.21100巴比妥钠盐分子量=206.18;0.04M溶液为&25克/升10.Tris-盐酸缓冲液（0.05M,25C）50毫升0.1M三羟甲基氨基甲烷（TriS溶液与X毫升0.1N盐酸混匀后，加水稀释至100毫

26、升。pHx（毫升）pHx（毫升）7.1045.78.1026.27.2044.78.2022.97.3043.48.3019.97.4042.0&4017.27.5040.38.5014.77.6038.58.6012.47.7036.68.7010.37.8034.58.80&57.9032.08.907.08.0029.2HOCH2NH2三羟甲基氨基甲烷（TriSH0CH2CH20H分子量=121.14;0.1M溶液为12.114克/升。Tris溶液可从空气中吸收二氧化碳，使用时注意将瓶盖严。11.硼酸-硼砂缓冲液（0.2M硼酸根）pH0.05M硼砂（毫升）0.2M硼砂（毫升）pH0.05

27、M硼砂（毫升）0.2M硼酸（毫升）7.41.09.0&23.56.57.61.5&5&44.55.57.82.0&0&76.04.0&03.07.09.0&02.0硼砂Na2B40720,分子量=381.43;0.05M溶液（=0.2M硼酸根）含19.07克/升。硼酸H2BO3,分子量=61.84,0.2M溶液为12.37克/升。硼砂易失去结晶水，必须在带塞的瓶中保存。11.硼酸-硼砂缓冲液（0.2M勺硼酸根）pH0.05M硼砂（毫升）0.2M硼酸（毫升）pH0.05M硼砂（毫升）0.2M硼酸（毫升）7.41.09.0&23.56.57.61.5&5&44.55.57.82.0&0&76.04

28、.0&03.07.09.0&02.0硼砂Na2B40710H20，分子量=381.43;0.05M溶液（=0.2M硼酸根）含19.07克/升。硼酸H2BO3,分子量=61.84,0.2M溶液为12.37克/升。硼砂易失去结晶水，必须在带塞的瓶中保存。12.甘氨酸-氢氧化钠缓冲液（0.05M）X毫升0.2M甘氨酸+Y毫升0.2Nna0H加水稀释至200毫升pHXYpHXY&6504.09.65022.4&8506.09.85027.29.050&810.05032.09.25012.010.45038.69.45016.810.65045.5甘氨酸分子量=75.07;0.2M溶液含15.01克/

29、升。13.硼砂-氢氧化钠缓冲液（0.05M硼酸根）X毫升0.05M硼砂+Y毫升0.2NNaOH加水稀释至200毫升pHXYpHXY9.3506.09.85034.09.45011.010.05043.09.65023.010.15046.0硼砂Na2B4O7OH2O,分子量=381.43;0.05M溶液为19.07克/升。14碳酸钠-碳酸氢钠缓冲液(0.1M)Ca2+、Mg2+存在时不得使用pH0.1MNa2CO3毫升）0.1MN2HCO3毫升）20C37C9.168.77199.409.12289.519.40379.789.50469.909.725510.149.906410.2810.

30、087310.5310.288210.8310.5791Na2CO20H20分子量=286.2;0.1M溶液为28.62克/升。N2HCO3分子量=84.0;0.1M溶液为&40克/升。15.“PBS”缓冲液PH7.67.47.27.0H2O100010001000100mlNaCl&5&5&5&5gNaHPO,2.22.22.22.2gNaHJO2410.10.20.30.4g十三、实验室中常用酸碱的比重和浓度名称分子式分子量比重面分浓度%(W/W)当量浓度(粗略)N配1升1N溶液所需毫升数盐酸HCl36.471.1937.212.0841.1835.411.81.1020.06.0硫酸h2

31、so498.091.8495.636.0281.1824.86.0硝酸HNO263.021.4270.9816.06321.4065.314.51.2032.366.1冰乙酸CH,COOH60.051.0599.517.459乙酸3CH3COOH98.06366.0磷酸3H2PO435.051.7185.015,30,45（依反应而定）67（以15N计）氨水NHOH0.9015670.90427.014.3700.9125.013.40.9610.05.6氢氧化钠溶液NaOH40.01.550.01953十四、硫酸铵饱和度的常用表1.调整硫酸铵溶液饱和度计算表（25C）硫整硫酸铵终浓文度,饱和

32、度10202530333540455055606570758090100每1升卜溶液加固体硫酸彳铵的克数硫酸铵初浓度05611414417619620924327731335139043047251656166276710578611813715018321625128832636540644949459269420295978911231551902252623003403824245206192530496193125158193230267307348390485583301930629412716219823527331435644954633124374107142177214252

33、2923334265229%饱和度353163941291642002381783194115064031639713216820524528537546945326599134171210250339431503366101137176214302392553367103141179264353603469105143227314653470107190275703572153237753611519880771579079在25C下，硫酸铵溶液由初浓度调到终浓度时，每升溶液所加固体硫酸铵的克数。2.调整硫酸铵溶液饱和度计算表（0匕）在0C硫酸:铵终浓度，饱和度202530354045505

34、56065707580859095100每100毫升溶液加固体硫酸铵的克数硫酸铵初浓度9%饱和度010.613.416.419.422.625.829.132.636.139.843.647.651.655.960.365.069.757.910.813.716.619.722.926.229.633.136.840.544.448.452.657.061.566.2105.3&110.913.916.920.023.326.630.133.737.441.245.249.353.658.162.7152.65.4&211.114.117.220.423.727.130.634.338.142

35、.045.050.354.759.22002.75.5&311.314.317.520.724.127.631.234.938.742.746.951.255.72502.75.6&411.514.617.921.124.528.031.735.539.543.647.852.23002.85.6&611.714.818.121.424.928.532.336.240.244.548.83502.85.7&711.815.118.421.825.429.132.936.941.045.34002.95.8&912.015.318.722.225.829.633.537.641.84502.95

36、.99.012.315.519.022.626.330.234.238.35003.06.09.212.515.919.423.026.830.834.85503.16.29.512.916.419.723.527.331.36003.16.39.713.216.820.123.127.96503.16.39.713216.820.524.47003.26.59.913.417.120.97503.26.610.113.717.48003.36.710.313.98503.46.810.59003.47.09503.51000在0C下，硫酸铵溶液由初浓度调到终浓度时，每100毫升溶液所加固体硫

37、酸铵的克数。卜五、常见蛋白质分子量参考值（单位：dalton）蛋白质分子量肌球蛋白myosin220,000甲状腺球蛋白thyroglobulin165,000B乳糖苷酶0-galactosidase130,000副肌球蛋白paramyosin100,000磷酸化酶aphosphorylasea94,000血清白蛋白serumalbumin68,000L-氨基酸氧化酶L-aminoacidoxidase63,000地氧化氢酶catalase60,000内酮酸激活酶pyruvatekinase57,000谷氨酸脱氢酶glutamatedehydrogenase53,000亮氨酸氨肽酶glutam

38、aedehydrogenase53,000Y-球蛋白，H链y-globulin,Hchain50,000延胡索酸酶（反丁烯二酸酶）fumarase49,000卵白蛋白ovalbumin43,000醇脱氢酶(肝)alcoholdehydrogenase(liver)41,000烯醇酶enolase41,000醛缩酶aldolase40,000肌酸激酶creatinekinase40,000胃蛋白酶原pepsinogen40,000D-氨基酸氧化酶D-aminoacidoxidase37,000醇脱氢酶(酵母)alcoholdehydrogenase(yeast)37,000甘油醛磷酸脱氢酶dly

39、ceraldehydephosphatedehydrogenase原肌球蛋白tropomyosin乳酸脱氢酶lactatedehydrgenase胃蛋白酶pepsin转磷酸核糖基酶phosphoribosyltransferase天冬氨酸氨甲酰转移酶，C链aspertatetranscarbamylase,Ccha羧肽酶AcarboxypeptidaseA碳酸酐酶carbonicanhydrase枯草杆菌蛋白酶subtilisinY吐球蛋白，L链y-blobulin,Lchain糜蛋白酶原（胰凝乳蛋白酶原）chymotrypsinogen胰蛋白酶trypsin木瓜蛋白酶（羧甲基）papain（

40、carboxymethyl）B-乳球蛋白0-lactoglobulin烟早化叶病毒外壳蛋白（TWV外壳蛋白）TWVcoatprotein肌红蛋白myoglobin天门冬氨酸氨甲酰转移酶，R链aspartatetranscarbamylase,Rc血红蛋白h（a）emoglobinQ0外壳蛋白Q0coatprotein溶菌酶lysozymeR7外壳蛋白R7coatprotein核糖核酸酶ribonuclease或RNase细胞色素CcytochromeC糜蛋白酶（胰凝乳蛋白酶）chymotrypsin36,00036,00036,00035,00035,000in4,00034,00029,00

41、027,60023.50025.70023.30023,00018,40017.50017,200h1i,0015.50015,00014.30013,75013.70011.70011,000或13,000十六、常见蛋白质等电点参考值（单位：pH）蛋白质等申占鲑精蛋白salmine12.1鲱精蛋白clupeine12.1鲟精蛋白sturline11.71胸腺绢蛋白thymohistone10.8珠蛋白（人）globin（human）7.5卵白蛋白ovalbuin4.71;4.59伴清蛋白conalbumin6.8;7.1血清白蛋白serumalbumin4.7-4.9肌清蛋白myoalbum

42、in3.5肌浆蛋白myogenA6.3B-乳球蛋白0-lactoglobulin5.1-5.3卵黄蛋白livetin4.8-5.0Y1球蛋白(人)Y-globulin(human)5.8;6.6Y2球蛋白(人)Y2-globulin(human)7.3;8.2肌球蛋白AmyosinA5.2-5.5原肌球蛋白myosinA5.1铁传递蛋白siderophilin5.9胎球蛋白fetuin3.4-3.5血纤蛋白原fibrinogen5.5-5.8a-艮晶体蛋白a-crystallin4.80-艮晶体蛋白0-crystallin6.0花生球蛋白arachin5.1伴花生球蛋白conarrachin3

43、.9角蛋白类keratins3.7-5.0还原角蛋白keratein4.6-4.7胶原蛋白collagen6.5-6.8鱼胶ichthyocol4.8-5.2白明胶gelatin4.7-5.0a-酷蛋白a-casein4.0-4.10酷蛋白0-casein4.5Y-酷蛋白Y-casein5.8-6.0a-卵清粘蛋白a-ovomucoid3.83-4.41a-粘蛋白amucoprotein1.8-2.7卵黄类粘蛋白vitellomucoid5.5尿促性腺激素urinarygonadotropin3.2-3.3溶菌酶lysozyme11.0-11.2肌红蛋白myoglobin6.99血红蛋白（人）

44、hemoglobin（human）7.07血红蛋白（鸡）hemoglobin（hen）7.23血红蛋白（马）hemoglobin（horse）6.92血蓝蛋白1hemerythrinl466.4蚯蚓血红蛋白chlorocruorin5.6血绿蛋白chlorocruorin4.3-4.5无脊椎血红蛋白erythrocruorins4.6-6.2细胞色素CcytochromeC9.8-10.1视紫质rhodopsin4.47-4.57促凝血酶原激酶thromboplastin5.2a-旨蛋白a-lipoprotein5.50旨蛋白0Tipoprotein5.40-卵黄脂磷蛋白0-lipovitellin5.9无青黄化病毒turnipyellowvvirus3.75牛痘病毒vacciniavirus5.3生长激素somatotropin6.85催乳激素prolactin5.73胰岛素i