NCBI序列提交方法

1、NCBI序列提交方法NCBI提交序列有两大类方式，一个是批量提交一个是单个序列提交。下面说的就是单个序列提交方式，这是最新的提交方式，由于NCBI更新很快，也许过段时间又不一样了，主要是按钮位置可能会变。首先要注册一个自己的NCBI账号：点击此处注册然后登陆账号：在已登陆状态下点击Submit点击图中标记处选择GengBank：submissionvviiareiimKipUgunngeuiwitre叨siarTrysrrtslQuitKStartYoifknow“he传youwarmmgo.Seieccr.nohlblaReaseleclkxi选择：Banklt：Howtosubmitdat

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11、rexample-IS-rRNAgmalKITSiflNA,amoEqtb0s4iBkoraamslbakhor修府ent晒喇c既-youmWwusaiwwdMmBSlonwontKlealxreanmncnftirynurssqucncEsennlcarkalEdfoidenotnuireadvancedsequenceanasisiQDb：U归Tr日IE#FEFNAjfeffiT与T口muluru?K：fTHai由U%IKUIM-1点击NewSub,下面是你提交的记录，即使你提交过程中中断了，也可以Continue!以下是具体提交过程：一.填写基本信息。口EGfWepenceSequcfr

12、rrgTechnohgMudK*deCigamsinSdimisswnCak?gQySojfcMSubmigion#1895945ContactInformatinrFirstName1khuphji|LastName|nu日啊|iDpjirtmentlenuzhou加”遇啊IhitituteriiSchoolafBio&FoodEnqineerln-q凯setAMrm|Fen?feAvenue咽忙J1UZIK4J|Seal制FrBlnGe|AnnulWIPJPaEtSlCifide|23EK1DD|Cwntiy回inaPhone1820515411:|山面邈逾械空匠ooi-KO-DOWMio

13、nab,*加EmailIncilhMprovideanemailaddrstoensure-1hatmessagesantrwewerlAtterruleEmil.图中1是作者名字，可以添加作者名，First和LastName,填了就可以，其他可以不填。.图中2是填是否出版，有三个选项：未出版，正在出版，已经出版。需要注意的是，如果选则未出版，需要在Title中注明，否则无法提交。.可以添加作者，然后Continue即可。SequenceAuthor;FirstNamewii。加inmai同mtN&mesunixRemoveAAA|AA口因/dc|moresequenceauthors.Ref

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15、Howmanydyouintendtas#ndinthis,submission?OSPIP2-1acaagctctgCgctcagctaagctctccaccattgcttgcaaagctgaGTCGTGTCACTCCTCCCGTTMGCTTCCCGCGGCGGCGAGAGCMGCTAAGCTAGGTCGGGCATGGGGAAGGACGAGGTGATGGAGAGCGGCGGCGCCGCCGGCGAGTTCGCGGCCAAGGACTACACGGACCCGCC7五填写你上传序列所属物种。然后即使继续。六选择你要你的序列式原始序列，就是你自己测序的序列，还是为已有序列做注释。然后继续。GenBan

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17、ntaclICopyriulit憎clairnfi=tcnsiGentertcw5chnoloarKInftrrnifltion.us电力。随1HtfgrjoriMedkeUDRgEKPiW,日时莺3dimUSA2CSQ4七序列信息填写你基因所属细胞器填写你对基因的注释，可以添加选项，根据自己实际情况添加。最下面可以添加你的PCR引物，然后继续，会让你检查，你检查后继续即可。benUankSubmissionsContaclRetefenceSequencingrecnnoicgkNucleotKleQig0nlsm刖EiswonCategorysourceMoflitHSubmission#

18、1885945SourceModifiersSourceOrganelleiTaDaticinInfDrmationChooðeMnret.t0gariEiieolocal町n.rfapplicablernMizhandirianSourceMladahersChoosesourcemodiner(s)andentervaiiesherePleaserefErtoSchjiteEodif旧rliffordescnphnnandfcrmatspurcerngdifitrv削口向/mov,BiOm矶旧7haet|为除idanother占口M匚日modifierAnolhersourcerel

19、aledintcfniation?网ForexampleTaKonomirClaEsifncatkinorLineageforunftnjown/unirECognizedargani&msYoushouldcrsaku&enote1asmeheaderlabel.D。netenterindruidualsourcemodiilers-asplaintexlhere.ChooselhemTrotITyouwanttoincludePCRprimersi向yoursubmission,theckh父已ennMniiid八信息补充填加序列信息，可以上传文件，也可以选项填写，建议用选项。选择你的信息

20、，可以添加，然后继续Ples&epirovidefeatureannotationsfcryQursubmissicntychoosingomeofth-etwoQAddfeaturesbyuploadmgnvecolumnfeaturetablefie*Addfeatures地，compllelflnginputformsThismethodis&estsuitablefor:asinglefeatureorafewteaturesappliedonasinglesequencethesamesinglefeatureorthesamefewfea1ure-&appliedtoallsequ

21、encesirFEdtue：5canbeaddedacrossanentiresequence口byspecificintervalswitoneormorequalifierscanbechosentoapplytoeachfeature,ifitisnotelecToaddafeltire.selectfeature匚alEqciryndtearturelypewithinthartcategotti：,codingRegkn(CDS)/GeneJmRNAityoursequenceencodesaprotein.X1RNA(rRNA,tRNA,non-codongRNA,mi5C_RNA

22、,etc)|EESSEng曰iRg7ORepeatregion(forsequencere-pe-ats,mobileelementsandsatelllrtes)ORegulatoryfeature(promoter,TATA_signal,RBSetc.)OOtnerAddAddedFeaturesforedining/irerricivalNofeaturesenteredyel|Cdnlind&在上图中选择添加选项，会让你填写模板是正链还是负链，如果是部分序列，是哪一端，产物是指你把序列通过BlastP比对后的到的序列号(qualifiers)和相似度(Value),然后Accept。跳