经皮植入记忆合金自体膨胀支架体外研究课件

1、Implantation of Endothelial Progenitor Cells on a Self-expanding Metal Stent: an in vitro StudyHongjian Shi,MDGaojun Teng,Ph.D,MDAihong Cao,MDDeparment of Radiology, Zhongda Hospital, Southeast Universty9/13/20222008 CSIR Beijing Hongjian SHIContentBackgroundMaterials and methodsResultsDiscussion9/1

2、3/20222008 CSIR Beijing Hongjian SHIBackgroundRestenosis:pseudointimal hyperplasia ( PTA, stent)High restenosis in TIPS(30%-50%, 1y)Bile leakage unavoidable during TIPSBile inhibit both SMC and ECCovered Viattor stent 1. Coldwell D, Radiology 1995 2. Zhuang Z,AJR 2002 3. LaBerge J,JVIR 1993 4. Nishi

3、mine K,Radiology 1995 5. Teng G,Radiology 1998;2005 6. Cejna M,Radiology 2001 7. Hausegger K,JVIR 20049/13/20222008 CSIR Beijing Hongjian SHIBackground EPC(endothelial progenitor cell) can differentiate into EC(endothelial cell) and incorporate into sites of active angiogenesis. Asahara, Science 199

4、79/13/20222008 CSIR Beijing Hongjian SHIShantsila E, et al.JACC 20079/13/20222008 CSIR Beijing Hongjian SHIZammarettia P, Int J Biochem Cell Biol 2005; 37: 493-503.9/13/20222008 CSIR Beijing Hongjian SHIEPC Isolation,Culture,IdentificationHealthy domestic farm swine(25kg)Density gradient centrifugat

5、ionCulture medium- EGM-2Cell markers CD34, VEGFR-2, VwfSpecific function: uptaken DiI-AC-LDL,and binding FITC-UEA-1 Ben-Shoshan, PharmacologyTherapeutics 2007 9/13/20222008 CSIR Beijing Hongjian SHIEPC uptaken DiI-Ac-LDLEPC binding FITC-UEA-19/13/20222008 CSIR Beijing Hongjian SHIImmunocytochemistry

6、 EPC cell markers CD34, KDR,vWF9/13/20222008 CSIR Beijing Hongjian SHIFibrinogen Preparation15 ml peripheral blood from the same animalCryoprecipitated technique Centrifugation at 1250 rpm at 4 for 20 min the plasma frozen to 70, stored at -20 4 refrigerator centrifugation at 2500 rpms at 2 for 40 m

7、in 1.5ml concentrated fibriongen DePalma L, Transfusion 19939/13/20222008 CSIR Beijing Hongjian SHIDiscussionIt is feasible to devise an EPC-seeded stent in vitroApplicabe in TIPS as well as vascular systemEPC-seeded stent with photocured-gelCD34 antibody capture EPC Stent 1 Shirota T, Biomaterials 2003 2 Aoki J, JACC 20059/13/20222008 CSIR Beijing Hongjian SHIAcknowlegementThis study was supported by grant 30670604 of the national natural science foundation of ChinaStents were sponsored by( Microtech Co.,Nanjing)Thanks for my collegues of Jiangsu Province key Lab of Molecular Imaging and