大数据时代的组学研究

1、大数据时代的组学研究大数据时代的组学研究“Traditional” VS “Modern” ways“Traditional” VS “Modern” waysBreakthrough of sequencing technologies1st : Sanger 2nd : Illumina Solexa; Roche 454; ABI SOLID3rd : PacBio SMRT; Oxford NanoporeBreakthrough of sequencing tecHuman Genome Project(HGP)30亿美元30亿个碱基对1984199020032015Human Ge

2、nome Project(HGP)30亿美The nations of the world must see that the human genome belongs to the worlds people, as opposed to its nations.- James D. WatsonThe nations of the world must The 1000 Genomes ProjectThe 1000 Genomes ProjectThe 1000 Genomes ProjectThe 1000 Genomes ProjectAll organisms have a gen

3、omeAll organisms have a genomePlants&AnimalsPlantsTree of LifeTree of LifeGenome always be the initial understanding of a speciesGenome always be the initial uLife is complicated and systematic Life is complicated and system中心法则 & Omics基因组De novo 测序Re-sequencingMetagenomicsEpigenomics转录组mRNAlncRNASm

4、all RNADegradome蛋白组ProteomeMetabolomeATCG中心法则 & Omics基因组De novo 测序Re-seDe novo genomeDe novo genome大数据时代的组学研究大数据时代的组学研究大数据时代的组学研究大数据时代的组学研究Comparative genomicsSynteny analysis Gene family expansion and subtraction Diversity between species Genome rearrangement Chromosome fusion and fission Ultra con

5、served elements Accelerative evolving regions Phylogenomics Comparative genomicsSynteny anGene expansion in the yak genomesensory perception energy metabolism Gene expansion in the yak geno大数据时代的组学研究大数据时代的组学研究大数据时代的组学研究Population genomicsPopulation genomicsWild yakDomestic yakPopulation structureWil

6、d yakDomestic yakPopulationSelection sweeps log-ratio0.65 and FST 0.17182 regions (14.5Mb, 0.54% of the genome)209 genesOverrepresented GO:Regulation of transmission of nerve impulse (GO:0051969)Arc, ASPA, ATP2B2, MYO6, NTRK2, Rab40c, SNCA, and TG30 genes involved in Neurogenesis and behaviorOnly a

7、few genes subject to physical characteristics and economically significant traitsEarly Domestication StageA trade-off between survival of yaks in a harsh high-altitude environment and performance under pastoral conditionsSelection sweeps log-ratio0.Demographic history inferred from SFSDomestication

8、of yak Human colonization in QTP Present2nd population expansionsbarley cultivation1st population expansionssettle and establish villages(agricultural settlements)5,20010,0007,0004,0003,000Demographic history inferred fMetagenomicsMetagenomicsEpigenomicsTwins Expression regulationGenomic imprintingTissue specific gene expressionDiseases relatedEpigenomicsTwins Expression reTranscriptomeTranscriptomeProteome & MetabolomeProteome & MetabolomeSequencing Costs and DevelopmentSequencing Costs and DevelopmeTen highest-grossing drugs in t