免疫荧光分型指南共41张课件

1、免疫荧光分型指南丁桃加拿大公共卫生署国家HV免疫实验室昆明20193OUREI免疫荧光分型指南概要样品处理单克隆抗体组滴定补偿荧色物绝对数校准荧光球通用分析模板OUREI概要样品处理溶解免洗的方法单克隆50-100全血溶解液抗体孵化10/15分钟孵化10/15分钟流式细胞仪分析OUREI样品处理建议加入2%的多聚甲醛固定绝对记数时荧光球和血液移液的准确性加过荧光球的样品,必须在当天上机分析在上机采集数据时,应在达到至少1微升血样之后才能停止;即如果荧光球的浓度为每微升1000,应收集到至少1000个荧光球才能停止采集OUREI建议一抗体组合OUREI一抗体组合不同的抗体组合双色:Facscou

2、nt and Guava: CD3/CD4PLG: CD45/4三色:BD and BC: CD4/3/45. CD4/8/3四色:BD and BC: CD4/8/3/45OUREI不同的抗体组合应使用哪种抗体组合?CD4/CDS/CD3CD16/CD19/CD3CD45/CD4CD45/CDSCD45/CD3/CD4CD45/CD3/CD8CD4应使用哪种抗体组合?NIH AND CDC指南Cytometry IClinical CytometryOriginal ArticlesUse of CD45 Gating in Three and Four-Color FlowCytometr

3、ic Immunophenotyping: Guideline From TheNational Institute of Allergy and Infectious Diseases,Division of aidsCarol T. Schnizlein-Bick, Francis F. Mandy, Maurice R.G. OGorman, Helene Paxton.Janet K-A. Nicholson, Lance E Hultin, Rebecca S GelmanCynthia L wilkening, and Daniella LivnarVoL. 52/RR-2Reco

4、mmendations and ReportsGuidelines for Performing Single-Platform Absolute CD4* T-CellDeterminations with CD45 Gating for Persons Infectedwith Human Immunodeficiency virusFrancis F. Mandy, PhDJa stk a nelson mOUREINIH AND CDC指南cD45的使用CD45可标记所有的白细胞CD45对淋巴细胞的表达大大高于单核细胞和巨噬细胞,故用来识别淋巴细胞OUREIcD45的使用cD45异相门

5、控:一个稳固的方法样品年龄cD45/ss异相门FS/SS均相门天CD4 FIT(4天Schnizlein-Bick et al. 2019OUREIcD45异相门控:一个稳固的方法免疫荧光分型指南共41张课件免疫荧光分型指南共41张课件免疫荧光分型指南共41张课件免疫荧光分型指南共41张课件免疫荧光分型指南共41张课件免疫荧光分型指南共41张课件免疫荧光分型指南共41张课件免疫荧光分型指南共41张课件免疫荧光分型指南共41张课件免疫荧光分型指南共41张课件免疫荧光分型指南共41张课件免疫荧光分型指南共41张课件免疫荧光分型指南共41张课件免疫荧光分型指南共41张课件免疫荧光分型指南共41张课件免疫荧光分型指南共41张课件免疫荧光分型指南共41张课件免疫荧光分型指南共41张课件免疫荧光分型指南共41张课件免疫荧光分型指南共41张课件免疫荧光分型指南共41张课件免疫荧光分型指南共41张课件免疫荧光分型指南共41张课件免疫荧光分型指南共41张课件免疫荧光分型指南共41张