Synucleozid-hydrochloride-NSC-377363-hydrochloride-DataSheet-生命科学试剂-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•Agonists•ScreeningLibrarieswww.MedChemESynucleozidhydrochlorideCat.No.:HY-135902ASynonyms:NSC377363hydrochloride分⼦式:C₂₂H₂₁ClN₆分⼦量:404.9作⽤靶点:DNA/RNASynthesis作⽤通路:CellCycle/DNADamage储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验H2O:2mg/mL(4.94mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM2.4697mL12.3487mL24.6975mL5mM---------10mM---------请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存⽅式和期限。BIOLOGICALACTIVITY⽣物活性Synucleozid(NSC377363)可编码α-突触核蛋⽩的SNCAmRNA的有效抑制剂(IC50=1.5μM)。Synucleozid选择性靶向α-synucleinmRNA的5'UTR的IRE位点，减少了加载到多核糖体中的SNCAmRNA的量，从⽽抑制了α-synuclein蛋⽩的翻译。Synucleozid有潜⼒⽤于帕⾦森病的相关研究。IC50&TargetIC50:1.5μM(SNCAIRERNA)[1]体外研究Synucleozid(0.25-1μM;24hours)abrogatescytotoxicityinducedbyα-synucleinpreformedfibrils,whichact1/2MasterofSmallMolecules—您⾝边的抑制剂⼤师www.MedChemEasseedsandrecruitendogenousα-synucleintoaggregate[1].Synucleozid(0-1μM;24hours)bindstotheAbulgenearthebaseoftheIREhairpin,reducedlevelsofα-synucleininadose-dependentmannerwithanIC50of500nM,andinhibitsα-synucleinproteinexpressioninSH-SY5Yneuroblastomacells[1].Synucleozid(100nM-100μM;24hours)bindsto2-AP-labeledandnativeIRERNAwithsimilaraffinities.Itdecreases2-APemissionwithanEC50valueof2.7μM,recoveryof2-APemissionsisobservedasafunctionofunlabeledSNCAIRERNA(RNA-0)concentration,affordingacompetitiveKdof1.5μM[1].Synucleozid(0.25-1μM;24hours)decreasesα-synucleinandotherproteinsthathaveIREsintheirmRNA’sUTRincludingAPP,PrP,FerritinandTfRasadose-dependentmannner.AllpanelsiscompletedinSH-SY5Ycells,exceptforPrPproteinwhichisassessedinNeuro-2Acells[1].CellViabilityAssay[1]CellLine:SH-SY5YneuroblastomacellsConcentration:0.25μM,0.5μM,1μMIncubationTime:24hoursResult:DecreasedLDHreleaseasadose-dependentmanner.WesternBlotAnalysis[1]CellLine:SH-SY5YneuroblastomacellsConcentration:0.25μM,0.5μM,1μMIncubationTime:24hoursResult:Decreasedα-synucleinexpressionasaconcentration-dependentmanner.REFERENCES[1].ZhangP,etal.Translationoftheintrinsicallydisorderedproteinα-synucleinisinhibitedbyasmallmoleculetargetingitsstructuredmRNA.ProcNatlAcadSciUSA.2020Jan21;117(3):1457-1467.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.For