Oligomycin-A-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•Agonists•ScreeningLibrarieswww.MedChemEOligomycinACat.No.:HY-16589CASNo.:579-13-5分⼦式:C₄₅H₇₄O₁₁分⼦量:791.06作⽤靶点:ATPSynthase;Fungal;Antibiotic作⽤通路:MembraneTransporter/IonChannel;Anti-infection储存⽅式:Powder-20°C3yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:100mg/mL(126.41mM;Needultrasonic)扫描⼆维码，H2O:<0.1mg/mL(insoluble)运⽤溶解⽅案计算器获得适合您实验体系的溶解⽅案MassSolvent1mg5mg10mgConcentration制备储备液1mM1.2641mL6.3206mL12.6413mL5mM0.2528mL1.2641mL2.5283mL10mM0.1264mL0.6321mL1.2641mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存⽅式和期限。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶1.请依序添加每种溶剂：10%DMSO40%PEG3005%Tween-8045%salineSolubility:≥2.08mg/mL(2.63mM);Clearsolution此⽅案可获得≥2.08mg/mL(2.63mM，饱和度未知)的澄溶液。以1mL⼯作液为例，取100μL20.8mg/mL的澄DMSO储备液加到400μLPEG300中，混合均匀；向上述1/3www.MedChemEwww.MedChemE2.体系中加⼊50μLTween-80，混合均匀；然后继续加⼊450μL⽣理盐⽔定容⾄1mL。请依序添加每种溶剂：10%DMSO90%(20%SBE-β-CDinsaline)Solubility:2.5mg/mL(3.16mM);Suspendedsolution;Needultrasonic此⽅案可获得2.5mg/mL(3.16mM)的均匀悬浊液，悬浊液可⽤于⼝服和腹腔注射。以1mL⼯作液为例，取100μL25.0mg/mL的澄DMSO储备液加到900μL20%的SBE-β-CD⽣理盐⽔⽔溶3.液中，混合均匀。请依序添加每种溶剂：10%DMSO90%cornoilSolubility:2.08mg/mL(2.63mM);Clearsolution;Needwarming此⽅案可获得2.08mg/mL(2.63mM)的澄溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。以1mL⼯作液为例，取100μL20.8mg/mL的澄DMSO储备液加到900μL⽟⽶油中，混合均匀。BIOLOGICALACTIVITY⽣物活性OligomycinA从链霉属中得到的⼀种线粒体F0F1-ATPase抑制剂，Ki值为1μM；OligomycinA具有抗真IC50&TargetKi:1μM(F0F1-ATPase)[1]体外研究OligomycinAisamitochondrialF0F1-ATPaseinhibitorwithaKiof1μM.OligomycinAshowscytotoxictotheNCI-60celllines,withGI50of10nM.OligomycinAalsoinhibitstheactivityofTritonX-100-solubilizedATPasewithaKiof0.1μM.Furthermore,OligomycinAhasanti-fungalactivity[1].OligomycinA(2.4ꢀµM)inhibitstheabilityofspermatozoatoachievefeasibleinvitrocapacitation(IVC),andalsosuppressesprogesterone-inducedinvitroacrosomeexocytosis(IVAE)aswellastheconcomitantpeaksofO2consumptionandATPlevels[2].PROTOCOLCellAssay[1]Drug(OligomycinA,etc.)dilutionsareaddedtomonolayerorsuspensioncellsin96wellplatesintriplicateforvaryingtimes.MTTisthenaddedtothewellsatafinalconcentrationof0.5mg/mL.SupernatantisremovedafterpelletingthereducedMTTcrystals.Thecrystalsarefullydissolvedin40mMHClinisopropanol.Platesarescannedonamicroplatereaderat595nm[1].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.户使⽤本产品发表的科研⽂献•NutrCancer.2020;72(4):696-707.•CellPhysiolBiochem.2018;48(6):2318-2336.Seemorecustomervalidationsonwww.MedChemEREFERENCES2/3www.MedChemEwww.MedChemE[1].SalomonAR,etal.Apoptolidin,aselectivecytotoxicagent,isaninhibitorofF0F1-ATPase.ChemBiol.2001Jan;8(1):71-80.[2].Ramió-LluchL,etal.OligomycinA-inducedinhibitionofmitochondrialATP-synthaseactivitysuppressesboarspermmotilityandinvitrocapacitationachievementwithoutmodifyingoverallspermenergylevels.ReprodFertilDev.2014;26(6):883-97.McePdfHeight关注MCE中国公众号，

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