Lidocaine-hydrochloride-hydrate-Lignocaine-hydrochloride-hydrate-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemELidocainehydrochloridehydrateCat.No.:HY-B0185BCASNo.:6108-05-0Synonyms:Lignocainehydrochloridehydrate分⼦式:C₁₄H₂₅ClN₂O₂分⼦量:288.81作⽤靶点:SodiumChannel;MEK;ERK;NF-κB;Apoptosis作⽤通路:MembraneTransporter/IonChannel;MAPK/ERKPathway;StemCell/Wnt;NF-κB;Apoptosis储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY⽣物活性Lidocaine(Lignocaine)hydrochloridehydrate抑制涉及复杂电压和依赖性的钠通道(sodiumchannels)。Lidocainehydrochloridehydrate通过调节miR-145表达和进⼀步抑制MEK/ERK和NF-κB信号通路来减少胃癌细胞的⽣长，迁移和侵袭。Lidocainehydrochloridehydrate⼀种酰胺衍⽣物，可⽤于研究室性⼼律失常。IC50&TargetERKNF-κBMEK体外研究Lidocaine(Lignocaine)(10nM;48hours)decreasessignificantlycellproliferation[2].Lidocaine(1-10nM;24-72hours)inhibitscellviabilityandachievesthemostsuppressingeffectsattheconcentrationof10ꢀnMandtreatmenttime48ꢀhours[2].Lidocaine(10nM;48hours)increasessignificantlytheapoptoticcellrate[2].Lidocaine(10nM;48hours)down-regulatesCyclinD1andup-regulatesp21expressionsignificantly[2].CellProliferationAssay[2]CellLine:ThehumangastriccancercelllineMKN45Concentration:10ꢀnMIncubationTime:48hoursResult:Decreasedsignificantlycellproliferation.1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemECellViabilityAssay[2]CellLine:ThehumangastriccancercelllineMKN45Concentration:1,5and10ꢀnMIncubationTime:24,48,72hoursResult:InhibitedMKN45cellviability.ApoptosisAnalysis[2]CellLine:ThehumangastriccancercelllineMKN45Concentration:10ꢀnMIncubationTime:48hoursResult:Increasedsignificantlytheapoptoticcellrate.WesternBlotAnalysis[2]CellLine:ThehumangastriccancercelllineMKN45Concentration:10ꢀnMIncubationTime:48hoursResult:Down-regulatedCyclinD1andup-regulatedp21expressionsignificantly.体内研究Lidocaine(Lignocaine)causescompletelyreversibletailnerveblockinrats.Mechanicalnociceptionblockproducedbylidocainehassloweronsetandfasterrecoverycomparedwiththermalnociceptionblock[3].户使⽤本产品发表的科研⽂献•NatMethods.2021Jul;18(7):788-798.•JNeuroinflammation.2017Nov2;14(1):211.•StemCellResTher.2021Feb4;12(1):107.•DigDisSci.2021Jan12.•MolMedRep.2022May;25(5):150.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].CumminsTR,etal.Settingupfortheblock:themechanismunderlyinglidocaine'suse-dependentinhibitionofsodiumchannels.JPhysiol.2007Jul1;582(Pt1):11.2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE[2].SuiH,etal.Lidocaineinhibitsgrowth,migrationandinvasionofgastriccarcinomacellsbyup-regulationofmiR-145.BMCCancer.2019Mar15;19(1):233.[3].LiZ,etal.Evaluationoftheantinociceptiveeffectsoflidocaineandbupivacaineonthetailnervesofhealthyrats.BasicClinPharmacolToxicol.2013Jul;113(1):31-6.McePdfHeightCaution:Producthasnotbeenfullyvalidatedfor