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EXPERIMENTSIX

Mycoplasma

Spirochaete

FungusObjectives1.Morphologyofspirochaete,mycoplasmaandfungiundermicroscope2.Colonytraitsofmycoplasmaandfungi3.Examinationoffungiinavarietyofspecimens

ContentsObservethecolonytraitsofmycoplasmaObservethemorphologyofleptospira,treponemapallidumandborreliavincentiiTheserologictestsofT.pallidumObserveliveleptospiraunderdark-fieldmicroscopeObservethecolonyofmonocellularfungusandmulticellularfungus,andtheirmorphologyundermicroscopeObserveInk-stainingcryptococcusneoformansGram-stainingwithcandidaalbicanswordsLeptospira钩端螺旋体Mycoplasma支原体Treponemapallidum梅毒螺旋体Leptospirainterrogan问号钩端螺旋体Borreliavincenti奋森氏螺旋体Sliverimpregnationmethod镀银染色法Fungus真菌monocellularfungus单细胞真菌multicellularfungus多细胞真菌spore孢子,芽孢Hyphae菌丝Pseudohypha假菌丝Macroconidium大分生孢子Chlamydoconidium厚膜孢子Blastospore芽生孢子Arthrospore关节孢子Sporangiospore孢子囊孢子Cryptococcusneoformans新型隐球菌Candidaalbicans白假丝酵母菌Mycoplasmaakindofprokaryotewithoutcellwall,multipleshapesthesmallestmicroorganismwhichcanbegrownonartificialmediaprovidedwithsterolandotherspecialnutritionalorphysicalrequirements‘friedegg’colony1.ObservethecolonytraitsofmycoplasmaSpirochaeteBorrelia:Borreliaburgdorferi,Borreliavincenti…Treponema:TreponemapallidumLeptospira:Leptospirainterrogans

2.themorphologyofleptospira,treponemapallidumandborreliavincentiiTreponemapallidum

Syphilis:chronic,slowlyprogressivecongenitaloracquiredsyphilis

CongenitalsyphilisInterstitialkeratitisHutchinson’steethSaddlenosePeriostitisAvarietyofcentralnervoussystemanomaliesFontanasliverstainingPrimarystage10to60dayshardchancre硬下疳richinTP,infectioushighlyhealspontaneouslySecondarystage2-10weeksafterprimarystageredmaculopapularrashanywhereonthebodyrichinTP,infectioushighlyTertiarystage

severalyearslatergranulomatouslesionsinskin,bone,andliverdegenerativechangesinthecentralnervoussystemcardiovascularlesionsfewTP3.Theserologictestsofsyphilis

Non-TPantigen-testTRUST:(toluidineredunheatedserumregaintest,甲苯胺红不热血清试验)VDRLantigensuspendedinsolutioncontainingtoluidinered,couldformvisibleclumpswhencombiningwithreagininserumProcedures1.placeserumandTRUSTantigenin23-29℃watertobalanceforaminutebeforestartingtotest.2.addonedropofnegativeandpositivecontrol(50ul)respectivelytotwocirclesofareactivecard,thenextendevenly.3.addonedropofserumorplasmaofspecimentoanothercircle,thenextendevenly.4.shaketheantigengentlyandaddadropofantigentoeachcircle5.rotatethecardfor8

minutesandobservetheresultsLeptospiresObserveliveleptospiraunderdark-fieldmicroscopeBorreliavincentiFungusmonocellularfungus:yeastmulticellularfungus:filamentousfungus,moldsporeandhyphaSabouraudMedium,pH4.0-6.0,22-28/37°C

4.colonyofmonocellularandmulticellularfungus,theirmorphologyundermicroscopeFormofmonocellularfungiCryptococcusneoformansCryptococcusneoformans

5.Ink-stainingCandidaalbicans6.Gramstaining

Formofmulticellularfungisporehypha

theworkinthisclassdemonstrationtests:thecolonytraitsofmycoplasma,themorphologyofleptospira,treponemapallidumandborreliavincentii,liveleptospiraunderdark-fieldmicroscope,thecolony

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