Anisomycin-Flagecidin-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEAnisomycinCat.No.:HY-18982CASNo.:22862-76-6Synonyms:Flagecidin;WuningmeisuC分⼦式:C₁₄H₁₉NO₄分⼦量:265.31作⽤靶点:DNA/RNASynthesis;JNK;Bacterial;Apoptosis;Antibiotic;Parasite作⽤通路:CellCycle/DNADamage;MAPK/ERKPathway;Anti-infection;Apoptosis储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:≥50mg/mL(188.46mM)H2O:4mg/mL(15.08mM;ultrasonicandwarmingandheatto60°C)*"≥"meanssoluble,butsaturationunknown.MassSolvent1mg5mg10mgConcentration制备储备液1mM3.7692mL18.8459mL37.6918mL5mM0.7538mL3.7692mL7.5384mL10mM0.3769mL1.8846mL3.7692mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.5mg/mL(9.42mM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(9.42mM);Clearsolution3.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(9.42mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性Anisomycin⼀种有效的蛋⽩质合成抑制剂，通过抑制肽转移酶80核糖体系统⼲扰蛋⽩质和DNA合成。Anisomycin⼀种JNK激活剂，可增强磷酸化JNK。Anisomycin⼀种细抗⽣素。IC50&TargetJNKDNAsynthesis体外研究ToexaminewhetherJNKhasacoreroleincolistin-inducedneurotoxicityinPC-12cells,anSP600125(ahighlyselectiveinhibitorofJNK)andAnisomycin(apotentactivator)areusedinthisstudy.Inordertoselectanappropriateconcentration,PC-12cellsaretreatedwitharangeofSP600125(0-80μM)andAnisomycin(0-20μM)respectivelyfor24h.TheresultsshowthatthecellsviabilitysignificantlydecreasesbySP600125treatmentinaconcentration-dependentmanner,observedattheconcentrationsgreaterthan20μM(p[1].体内研究Anisomycin(60mg/kg;for4-weekcontinuousintravenousadministration)significantlydecreasesmousebodyweightinadose-relatedmanner,comparedwiththecontrolgroup.Anisomycin(15mg/kg;for4-weekcontinuousintravenousadministration)slightlyandtransientlydecreasesthemousebodyweight.Thereisnosignificantdifferenceofthemousebodyweightin5mg/kggroup[3].AnimalModel:Balb/cmiceofbothsexes(4-5weeksold)[3]Dosage:84,99,116,136or160mg/kg;0.2mLpermouseAdministration:IntravenouslyinjectedthroughmousetailveinResult:ThecalculatedLD50forAnisomycinwas119.64mg/kg.户使⽤本产品发表的科研⽂献•JHazardMater.2020Nov15;399:122809.•JExpClinCancerRes.2020Feb5;39(1):29.•CellRep.2021Jul20;36(3):109398.•CellDeathDis.2021Jan11;12(1):63.•Diabetes.2021Oct21;db210240.2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemESeemorecustomervalidationsonwww.MedChemEREFERENCES[1].LuZ,etal.Colistin-inducedautophagyandapoptosisinvolvestheJNK-Bcl2-BaxsignalingpathwayandJNK-p53-ROSpositivefeedbackloopinPC-12cells.[2].ZhengleTang,etal.InvivotoxicologicalevaluationofAnisomycin.ToxicolLett.2012Jan5;208(1):1-11.[3].GaoX,etal.TranscriptionalregulationofstresskinaseJNK2inpro-arrhythmicCaMKIIδexpressionintheagedatrium.CardiovascRes.2018Apr1;114(5):737-746.[4].SynthesisMarinaMacías-Silva,etal.AnisomycinisaMultifunctionalDrug:MorethanJustaTooltoInhibitProtein.CurrentChemicalBiology,2010,4,124-132.McePdfHeightCaution:Product