Penicillin-G-sodium-salt-Benzylpenicillin-sodium-salt-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEPenicillinGsodiumsaltCat.No.:HY-B1463CASNo.:69-57-8Synonyms:Benzylpenicillinsodiumsalt分⼦式:C₁₆H₁₇N₂NaO₄S分⼦量:356.37作⽤靶点:Bacterial;Antibiotic作⽤通路:Anti-infection储存⽅式:4°C,sealedstorage,awayfrommoisture*该产品在溶液状态不稳定，建议您现⽤现配，即刻使⽤。溶解性数据体外实验DMSO:≥100mg/mL(280.61mM)H2O:≥100mg/mL(280.61mM)*"≥"meanssoluble,butsaturationunknown.MassSolvent1mg5mg10mgConcentration制备储备液1mM2.8061mL14.0304mL28.0607mL5mM0.5612mL2.8061mL5.6121mL10mM0.2806mL1.4030mL2.8061mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现⽤现配，即刻使⽤.体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.5mg/mL(7.02mM);Clearsolution1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(7.02mM);Clearsolution3.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(7.02mM);Clearsolution4.请依序添加每种溶剂：PBSSolubility:100mg/mL(280.61mM);Clearsolution;NeedultrasonicBIOLOGICALACTIVITY⽣物活性PenicillinGsodiumsalt⼀种典型的β-内酰胺类抗⽣素。IC50&Targetβ-lactam体外研究TheUltraviolet-visible(UV-Vis)absorptionspectrumofthePenicillinGsodiumsalt-TEM-1systemismarkedlydifferenttothatofPenicillinGsodiumsaltandTEM-1β-lactamase,indicatingtheformationofnewcomplexesbetweenPenicillinGsodiumsaltandTEM-1β-lactamase.TheUV-VisabsorptionofTEM-1β-lactamaseincreasesandaslightred-shiftoccursastheconcentrationofPenicillinGsodiumsaltincreasing,indicatingthattheinteractionbetweenPenicillinGsodiumsaltandTEM-1β-lactamaseresultsinsubtleconformationalchangesofTEM-1β-lactamase[1].体内研究Inthelogisticregressionmodel,theprobabilityofapositiveswabinthecontrolgroupis1.6timeshigherthanthatinthepigstreatedwithPenicillinGsodiumsalt(P[2].PROTOCOLKinaseAssay[1]At278K,variousconcentrationsofPenicillinGsodiumsalt,cefalexinandcefoxitinsolutionsareaddedtoTEM-1β-lactamasesolution(5×10-6M).Theconcentrationsofthethreeantibioticsaregraduallyincreasedfrom0to25×10-6M.Followingmixingandinteractionfor2min,theUltraviolet-visible(UV-Vis)absorptionspectraarerecordedonaspectrophotometerwithaslitof2nmandscanningspeedof400nm/minusing0.02Mphosphatebuffer(pH7.0)asareference[1].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.AnimalArandomizedcompleteblockdesignwith2replicatesisusedforthisstudy.Eachreplicateincludes448Administration[2]pigs,with16pensand28pigsperpen.Pigsarealsosortedbyweight;suchthatanimalsofsimilarweightbasedonvisualobservationaregroupedtogetherwithinblocks.Twotreatmentsarerandomlyassignedwithineachblockof2contiguouspensusingaformalrandomizationprocess.ThetreatmentgroupsareControl(notreatmentgiven)andTreated(PenicillinGsodiumsalt).PenicillinGsodiumsaltisadministeredviathedrinkingwaterfor5dover2periodsoftreatment.Thefirsttreatmentperiodcommencesonthedayofweaning,whenthepigsaremovedintothenurserybarns(Day1)andendsonDay5.ThesecondtreatmentperiodbeginsonDay21andendsonDay25.TheControlgroupdoesnotreceivetreatment[2].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE户使⽤本产品发表的科研⽂献•Theranostics.2022Jan1;12(3):1187-1203.•EBioMedicine.2022Apr;78:103943.•Chemosphere.2019Jun;225:378-387.•Cancers(Basel).2022Sep28;14(19):4740.•ApplMicrobiolBiotechnol.2022Apr;106(7):2689-2702.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].YangJ,etal.SpectroscopicanalysisanddockingsimulationontherecognitionandbindingofTEM-1β-lactamasewithβ-lactamantibiotics.ExpTherMed.2017Oct;14(4):3288-3298.[2].ByraC,etal.DecreasedmortalityofweanedpigswithStreptococcussuiswiththeuseofin-waterpotassiumpenicillinG.CanVetJ.2011Mar;52(3)