Lin28-let-7a-antagonist-1-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemELin28-let-7aantagonist1Cat.No.:HY-100692CASNo.:2024548-03-4分⼦式:C₃₁H₂₉N₅O₇分⼦量:583.59作⽤靶点:MicroRNA作⽤通路:Epigenetics储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:100mg/mL(171.35mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM1.7135mL8.5677mL17.1353mL5mM0.3427mL1.7135mL3.4271mL10mM0.1714mL0.8568mL1.7135mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。BIOLOGICALACTIVITY⽣物活性Lin28-let-7aantagonist1对Lin28-let-7a的结合有明显的拮抗作⽤，其对Lin28A-let-7a-1结合的IC50值为4.03μM。IC50&TargetIC50：4.03μM(Lin28A-let-7a-1)[1].1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE体外研究Lin28-let-7aantagonist1(compound1)showsaclearantagonisticeffectagainsttheLin28A-let-7a-1interactionwithanIC50of4.03μM.Interestingly,Lin28-let-7aantagonist1alsoinhibitstheLin28B-let-7interaction,withslightlyreducedpotency.DicerprocessingassayrevealsthatLin28-let-7aantagonist1clearlyblockstheformationoftheLin28A-pre-let-7gcomplexandinducesmiRNAprocessing,resultinginthegenerationofmaturelet-7gmiRNA.WhentheexpressionofbothLin28AandLin28Bareknocked-downusingsiRNAinJARcells,theLin28-let-7aantagonist1-inducedincreaseincellularlet-7levelsisclearlyattenuated.Lin28A-let-7a-IN-1increasesmaturelet-7levelsinPA-1cellsthatexpresshighlevelofLin28A.Ontheotherhand,let-7levelsarenotaffectedby1inMCF7cellsthatrarelyexpressLin28proteins.Takentogether,Lin28-let-7aantagonist1specificallyinducestheincreaseinthecellularlet-7levelsbytargetingLin28[1].户使⽤本产品发表的科研⽂献•MolCell.2021Oct12;S1097-2765(21)00796-6.•Oncogenesis.2022Jul2;11(1):37.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].LimD,etal.DiscoveryofaSmall-MoleculeInhibitorofProtein-MicroRNAInteractionUsingBindingAssaywithaSite-SpecificallyLabeledLin28.JAmChemSoc.2016Oct7.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonl