Madrasin-DDD00107587-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEMadrasinCat.No.:HY-100236CASNo.:374913-63-0Synonyms:DDD00107587分⼦式:C₁₆H₁₇N₅O₂分⼦量:311.34作⽤靶点:Others作⽤通路:Others储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:2mg/mL(6.42mM;Needultrasonic)H2O:<0.1mg/mL(insoluble)MassSolvent1mg5mg10mgConcentration制备储备液1mM3.2119mL16.0596mL32.1192mL5mM0.6424mL3.2119mL6.4238mL10mM---------请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。BIOLOGICALACTIVITY⽣物活性Madrasin(DDD00107587)⼀种剪接抑制剂，可在体外阻⽌剪接中间体和产物的形成，并⼲扰剪接体组装途径中的⼀个或多个早期步骤。Madrasin还能在体外抑制pre-mRNA的剪接，并修饰细胞中内性pre-mRNA1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE的剪接。IC50&Targetpre-mRNAsplicing[1]体外研究Madrasin(10-30μM;4-24hours;HeLacells)treatmentinhibitsthesplicingofeachoftheRIOK3,BRD2,andHsp40,MCL1,CCNA2,AURKAandp27pre-mRNAsinbothHeLaandHEK293cells[1].Madrasin(10-30μM;4-24hours;HeLaandHEK293cells)treatmentshowsadose-andtime-dependentinhibitoryeffectoncellcycleprogression[1].RT-PCR[1]CellLine:HeLacellsConcentration:10μM,20μM,or30μMIncubationTime:4hours,8hours,or24hoursResult:Increasedininhibitionofpre-mRNAsplicing.CellCycleAnalysis[1]CellLine:HeLaandHEK293cellsConcentration:10μM,20μM,or30μMIncubationTime:4hours,8hours,or24hoursResult:Inthepresenceof10μM8hoursaftertreatment,theproportionofcellsinG2,M,andSphasesincreased,withaconcomitantdecreaseinthenumberofG1phasecells.Thiseffectincreasedovertime,with>40%ofcellsinG2andMphaseand>50%inSphase24hours.户使⽤本产品发表的科研⽂献•NucleicAcidsRes.2019Sep5;47(15):8239-8254.•Theranostics.2022Jul11;12(12):5451-5469.•JMolMed(Berl).2019Aug;97(8):1183-1193.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].AndreaPawelleketal.IdentificationofSmallMoleculeInhibitorsofPre-mRNASplicing.JBiolChem,2014Dec12,289(50):34683-34698.McePdfHeight2/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemECaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseo