ML133-hydrochloride-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEML133hydrochlorideCat.No.:HY-100230ACASNo.:1222781-70-5分⼦式:C₁₉H₂₀ClNO分⼦量:313.82作⽤靶点:PotassiumChannel作⽤通路:MembraneTransporter/IonChannel储存⽅式:4°C,sealedstorage,awayfrommoisture*Insolvent:-80°C,6months;-20°C,1month(sealed

storage,awayfrommoisture)溶解性数据体外实验DMSO:100mg/mL(318.65mM;ultrasonicandwarmingandheatto60°C)H2O:<0.1mg/mL(insoluble)MassSolvent1mg5mg10mgConcentration制备储备液1mM3.1865mL15.9327mL31.8654mL5mM0.6373mL3.1865mL6.3731mL10mM0.3187mL1.5933mL3.1865mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month(sealedstorage,awayfrommoisture)。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.08mg/mL(6.63mM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemESolubility:≥2.08mg/mL(6.63mM);Clearsolution3.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.08mg/mL(6.63mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性ML133hydrochloride⼀个选择性的Kir2家族通道的抑制剂，在pH7.4和pH8.5时的IC50值分别为1.8μM和290nM。IC50&TargetIC50:1.8μM(Kir2atpH7.4),290nM(Kir2atpH8.5)[1].体外研究ML133,whichinhibitsKir2.1withIC50of1.8μMatpH7.4and290nMatpH8.5,butexhibitslittleselectivityagainstothermembersofKir2.xfamilychannels[1].ML133hasnoeffectonKir1.1(IC50>300μM),anddisplaysweakactivityforKir4.1(76μM)andKir7.1(33μM),makingML133themostselectivesmallmoleculeinhibitoroftheKirfamilyreportedtodate[1].户使⽤本产品发表的科研⽂献•CellMetab.2022Sep7;S1550-4131(22)00359-X.•PharmacolRes.2022Feb2;177:106112.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].WangHR,etal.SelectiveinhibitionoftheK(ir)2familyofinwardrectifierpotassiumchannelsbyasmallmoleculeprobe:thediscovery,SAR,andpharmacologicalcharacterizationofML133.ACSChemBiol.2011Aug19;6(8):845-56.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearch