PCI-33380-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEPCI-33380Cat.No.:HY-100335CASNo.:1022899-36-0分⼦式:C₄₆H₅₂BF₂N₁₁O₃分⼦量:855.78作⽤靶点:Btk作⽤通路:ProteinTyrosineKinase/RTK储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:≥50mg/mL(58.43mM)*"≥"meanssoluble,butsaturationunknown.MassSolvent1mg5mg10mgConcentration制备储备液1mM1.1685mL5.8426mL11.6852mL5mM0.2337mL1.1685mL2.3370mL10mM0.1169mL0.5843mL1.1685mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%saline1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemESolubility:≥2.5mg/mL(2.92mM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(2.92mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性PCI-33380布鲁顿酪氨酸激酶(BTK)的选择性的、不可逆抑制剂(荧光探针)。IC50&TargetBTK[1][2].体外研究PCI-33380boundtoBtkcouldbedetectedbydenaturinggelelectrophoresisandfluorescentgelscanning[2].Immunofluorescence[2]CellLine:HumanBcells.Concentration:2μM.IncubationTime:1h.Result:Foundthat10nMofPCI-32765wassufficienttofullyoccupytheactivesiteofBtkinprimaryBcellsinculturebyusingthefluorescentlytaggedderivativePCI-33380.REFERENCES[1].ZuoY,etal.Anovel2,5-diaminopyrimidine-basedaffinityprobeforBruton'styrosinekinase.SciRep.2015Nov4;5:16136.[2].HonigbergLA,etal.TheBrutontyrosinekinaseinhibitorPCI-32765blocksB-cellactivationandisefficaciousinmodelsofautoimmunediseaseandB-cellmalignancy.ProcNatlAcadSciUSA.2010Jul20;107(29):13075-80.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400