RT-AM-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemERT-AMCat.No.:HY-108715ACASNo.:2280796-94-1分⼦式:C₂₆H₂₅N₃O₁₁分⼦量:555.49作⽤靶点:Others作⽤通路:Others储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:25mg/mL(45.01mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM1.8002mL9.0011mL18.0021mL5mM0.3600mL1.8002mL3.6004mL10mM0.1800mL0.9001mL1.8002mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。BIOLOGICALACTIVITY⽣物活性RT-AMrealthiol前体药物。RealThiol⼀种于可逆反应的荧光探针，可以定量监测活细胞中⾕胱⽢肽的实时动态变化。体外研究ToenhancethecellpermeabilityofRealThiol,thecarboxylicacidgroupsisconvertedtoacetoxymethyl(AM)esters,whicharereadilyhydrolysedbyesterasestoregenerateRealThiolinsidecells.Forcellstreatedwith1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEtheRT-AM,real-timeratiometricimagesoftherapidchangesofintracellularGSHconcentrationsinsinglecellscanbegeneratedbydividingthefluorescenceintensityvaluesforthe405ꢀnmchannelbythe488ꢀnmchannelateachcorrespondingpixel[1].PROTOCOLCellAssay[1]TheHeLacelllineisgrowninDMEMmediasupplementedwith10%FBSand1%1003PenStrep.Cellsareculturedunderacontrolledatmosphere(37°C,5%CO2).Glassbottomdishesareusedforcellcultureduetoconfocalscanningrequirements.CellsaretreatedwithRT-AM(1ꢀμMwith1%DMSOinDMEM)for10-15ꢀminbeforeimaging.Confocalimagesareacquiredwith405ꢀnmlaser/418–495ꢀnmfilterand488ꢀnmlaser/499–615ꢀnmfilter.Allthemicroscopesettingsarekeptconsistentineach[1].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.户使⽤本产品发表的科研⽂献•EnvironSciTechnol.2022Aug10.•EnvironPollut.2021Oct7;118296.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].JiangX,etal.Quantitativereal-timeimagingofglutathione.NatCommun.2017Jul13;8:16087.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Te