Menadione-bisulfite-sodium-Menadione-sodium-bisulfite-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEMenadionebisulfitesodiumCat.No.:HY-B1897ACASNo.:130-37-0Synonyms:Menadionesodiumbisulfite;VitaminK3sodiumbisulfite分⼦式:C₁₁H₉NaO₅S分⼦量:276.24作⽤靶点:Others作⽤通路:Others储存⽅式:4°C,sealedstorage,awayfrommoisture*Insolvent:-80°C,6months;-20°C,1month(sealed

storage,awayfrommoisture)溶解性数据体外实验DMSO:100mg/mL(362.00mM;Needultrasonic)H2O:≥100mg/mL(362.00mM)*"≥"meanssoluble,butsaturationunknown.MassSolvent1mg5mg10mgConcentration制备储备液1mM3.6200mL18.1002mL36.2004mL5mM0.7240mL3.6200mL7.2401mL10mM0.3620mL1.8100mL3.6200mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month(sealedstorage,awayfrommoisture)。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.5mg/mL(9.05mM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(9.05mM);Clearsolution3.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(9.05mM);Clearsolution4.请依序添加每种溶剂：PBSSolubility:120mg/mL(434.40mM);Clearsolution;NeedultrasonicBIOLOGICALACTIVITY⽣物活性Menadionebisulfitesodium⼀种可以诱导急性氧化应激的试剂，作为植物防御活化剂来抵抗病。体外研究Menadionesodiumbisulfite(500μM)treatmentandtheconcomitantROSincreasehaveanimportantimpactonthephotosyntheticactivityofthecells.GlobalandspecificantioxidantdefencesdecreasewithMenadionesodiumbisulfite(500μM)treatment[1].MenadionesodiumbisulfiteandPABAactivatesimilardefenseresponses(PR,ROS,andHR).Menadionesodiumbisulfiteenhancesresistance,bothlocallyandsystemically,tophomastemcankerdiseasecausedbyLeptosphaeriamaculans.Menadionesodiumbisulfitetreatmentinducesresistanceagainstdownymildewinpearlmillet.Menadionesodiumbisulfiteactsthroughprimingofspecificsetsoftheplantinnate-defenserepertoire.Menadionesodiumbisulfitetreatmentup-regulatestheexpressionofageneencodingaGRX480protein,amemberoftheglutaredoxinfamilythatregulatesproteinredoxstate[1].PROTOCOLKinaseAssay[1]Briefly,cellsarelysedbysonication(6cyclesof10sseparatedby30s,onice)inabuffercontaining25mMHepesatpH7.5,5mMMgCl2,5mMDTT,2mMPMSF,5mMEDTA,400mMsorbitoland10μg/mLProteaseInhibitorcocktail.Aquantityof40μgSymbiodiniumproteinextractisincubatedinthereactionbuffer.Themixtureconsistsof50μMAc-DEVD-AFC,100mMHepesatpH7.5,10%(v/v)sucrose,0.1%(v/v)CHAPS,10mMDTTand10mMPMSF.Samplesarethenincubatedinblack96-wellmicroplatesandthefluorescenceemittedismeasuredevery3minfor90minat505nm,witha400nmexcitationwavelengthprovidedbyaFLX-Xeniusspectrofluorometer.Caspase-likeactivitiesarethenexpressedinpmolofAFCcleavageperminuteandillustratedaspercentageofthecontrols.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.REFERENCES[1].RobertyS,etal.DifferentialantioxidantresponsebetweentwoSymbiodiniumspeciesfromcontrastingenvironments.PlantCellEnviron.2016Dec;39(12):2713-2724McePdfHeigtCaution:Producthasnot