龙游萎鼻讲座幻灯片

AtrophicRhinitisanditsControl

（猪萎缩性鼻炎及其控制）

—ARadicatorTM（克伟®）钱卫东AtrophicRhinitis–DiseaseandMethodsOutline:BriefoverviewofAtrophicRhinitis–thediseaseandassociatedorganismsDiagnosisEpidemiologyControlMethodsofAssessingAtrophicRhinitis萎缩性鼻炎—疾病与方法内容纲要：萎缩性鼻炎的简介-疾病与病原诊断流行病学控制评估萎缩性鼻炎的方法AtrophicRhinitis（萎缩性鼻炎）–Disease（疾病）TwoFormsofAtrophicRhinitisNon-Progressive,uncomplicatedBordetellabronchiseptica,toxigenicMildTurbinatesrecoverProgressivePasteurellamultocida,toxigenic,usuallyTypeDToxinistheactivefactorincausingatrophyMildtosevereremodelingofturbinatesandbonystructureofthenoseLesionprogressesinabsenceofdetectableorganismsinthenasalcavity萎缩性鼻炎的两种形式：

非进行性、非复合性-支气管败血波氏杆菌，产毒素的-发病较轻-鼻甲骨可再生恢复

进行性-产毒素多杀性巴氏杆菌，常是D型-毒素是导致萎缩的病因-轻度至严重地改变鼻甲骨形态和鼻骨组织的形态-当鼻腔检测不到病原时，病变持续存在AtrophicRhinitis（萎缩性鼻炎）–Disease（疾病）ProgressiveAtrophicRhinitisSeverityisvariable–Dependson:Ageofpig(youngeraremoresusceptible)Amountoftoxin(P.multocida)OtherirritantspromotingcolonizationoftoxigenicP.multocidaB.bronchiseptica

(naturalconditions)Aceticacid(experimentalconditions)Airquality?进行性萎缩性鼻炎严重程度不同，取决于：

#猪的年龄（年龄小的猪更敏感）#毒素量（多杀性巴氏杆菌）#其它有助于产毒素多杀性巴氏杆菌定居繁殖的刺激因素-支气管败血波氏杆菌（自然条件下）-乙酸（实验条件下）-空气质量？AtrophicRhinitis–DiseaseEconomicsEstimatesofprevalencerangefrom20%to80%Economiclossdueto:PoorGrowthPoorFeedConversionIncreasedmedicationcostsUnevenlotsforslaughter萎缩性鼻炎—疾病的经济影响

发病率约在20-80%经济损失包括：-生长不良-饲料转化率降低-药物治疗费用增加-屠宰猪大小不整齐ProgressiveAtrophicRhinitis–EpidemiologyHorizontalTransmissionFromintroducedcarrierpigstosusceptiblepenmatesVerticalTransmissionFromcarriersowstosusceptibleoffspring进行性萎缩性鼻炎—流行病学水平传播-从引入的带菌猪传给同圈的敏感猪垂直传播-从带菌母猪传给敏感的子代猪ProgressiveAtrophicRhinitis–ControlControlsourcesofinfectionsEarlyweaningwithmedicationAntibioticsinSow’sGestationFeedInjectableforsucklingpigs(repeateddoses)AntibiotictreatmentinolderpigsmaylimitseverityAvoidmixinganimalsofdiverseorigin,ages,sizesVaccinationSowPiglet进行性萎缩性鼻炎—控制

控制感染来源-药物性早期断奶#在怀孕母猪料中添加抗生素#对哺乳猪进行注射给药-对较大日龄猪进行抗生素治疗，以控制病的严重性-避免不同来源、不同日龄和不同大小猪的混养

接种疫苗-母猪-仔猪ProgressiveAtrophicRhinitis–ControlVaccinationSow–Usually2mLdose,twicebeforefirst farrowing；SingledosepriortosubsequentfarrowingsMaternalantibodyprotectssucklingpigletsMayhelpreducesheddingincarriersowsCombinedwithsegregatedweaning,maybeabletoeliminateARfromaherdPiglet–1mLto2mL(perlabel),twicearoundthetimeofweaning(riskofmaternalantibodyinterference)Both

进行性萎缩性鼻炎—控制

接种疫苗#母猪–每头份2毫升，第一次产仔前接种两次；以后产仔前接种一次-母源抗体保护仔猪-可减少带菌母猪的病菌排放-与隔离断奶结合，可以使猪群中的AR得以净化#仔猪–接种1-2毫升，在断奶前后接种两次（有母源抗体干扰的风险）#母猪和仔猪都接种疫苗AtrophicRhinitis–AssessmentNormal(正常)Atrophic(萎缩)DorsalTurbinateVentralTurbinateDorsalScrollVentralScroll萎缩性鼻炎—评估(背鼻甲)(腹鼻甲)(背卷曲)(腹卷曲)ProgressiveAtrophicRhinitis–AssessmentSlaughterCheckMultiplepigsfromherdCutsnoutcleanlyLevelof2ndpremolarPerpendiculartothehardpalateScoringSystems(Subjective/Objectiveranking)BoninessofturbinatesDistortionofnasalcavityandturbinatesAtrophyofturbinatesIncreasedairspace–measurewithcalipersor‘ringsizer’Scaleof0to5[Runnels,L.J.,VeterinaryClinicsofNorthAmerica,LargeAnimalPractice,4(2),Nov.1982,pp301-319.]进行性萎缩性鼻炎—评估屠宰检查

#每个猪群应检查多头猪#在鼻部做横切-第2前臼齿处横切-垂直于硬颚#评分系数（主观/客观评分）-鼻甲骨的骨质-鼻腔和鼻甲骨扭曲-鼻甲骨萎缩-鼻腔空隙增大，用卡钳或环尺测量-0-5分制ProgressiveAtrophicRhinitis–AssessmentScoringSystems(Subjective/Objectiveranking)Scaleof0to5[Runnels,L.J.,VeterinaryClinicsofNorthAmerica,LargeAnimalPractice,4(2),Nov.1982,pp301-319.]0=Normal–Turbinatesfillnasalcavity,septumissymmetricalandstraight1=SlightDeviationfromNormal2=SlightAtrophy-Increasedspace(upto6mm)inventralnasalcavity3=ModerateAtrophy–Moderatelydistortedseptum,7–8mmspaceinventralnasalcavity.Degenerativechangesinventralscrolls4=ModeratetoSevereAtrophy–Greaterthan8mmspacing.Degenerativechangesandlossofboneinscrolls,distortionofseptum,lossofventralscroll5=SevereAtrophy–Lossofventralturbinatesanddegenerativechangeorlossofdorsalturbinate.Severedeviationofseptum.进行性萎缩性鼻炎—评估

评分系统（主观/客观评分）-0-5分制0=正常–鼻甲骨充满鼻腔，鼻中隔对称和笔直1=轻度异常–与正常比较，稍有改变2=轻度萎缩–腹侧鼻腔空隙增大，最大至6mm3=中度萎缩–鼻中隔稍扭曲，腹侧鼻腔空隙增大至7-8mm。腹卷曲退行性改变4=中度至严重萎缩–鼻腔空隙增大至8mm以上。鼻甲卷曲退行性改变和骨质丢失，鼻中隔扭曲，腹卷曲消失5=严重萎缩–腹鼻甲消失和背鼻甲退行性改变或消失。鼻中隔严重扭曲ProgressiveAtrophicRhinitis–AssessmentVisualScoringExamples(目测评分实例)‘0’‘3’‘5’进行性萎缩性鼻炎—评估AtrophicRhinitis–AssessmentRing-sizerscoring-Measurespacebetweenthedorsalandventralturbinatesandthewallsofthenasalcavity-Sumof4measurements(mm)resultsgivesthefinalsnoutscore-Thelargerthescore,themoreseveretheturbinatedamage:<9mm=normalturbinates

10–20mm=mildturbinatedamage>21mm=severeturbinatedamage萎缩性鼻炎—评估

环尺评分法

-测量背腹鼻甲骨与鼻腔壁之间的距离

-4个位置测量值之和为鼻甲骨的评分值-评分值越大，鼻甲骨萎缩越严重<9mm=正常鼻甲骨10-20mm=轻度损伤的鼻甲骨>21mm=严重损伤的鼻甲骨AtrophicRhinitis–AssessmentRingSizerMethod（环尺法）Measurespacebetweenturbinateandnasalwall（测量鼻甲骨与鼻腔壁之间的距离）萎缩性鼻炎—评估AtrophicRhinitis–AssessmentRingSizer（环尺法）Measurespacebetweenturbinateandnasalwall（测量鼻甲骨与鼻腔壁之间的距离）萎缩性鼻炎—评估ProgressiveAtrophicRhinitis–AssessmentWheretoCut–2ndpremolar（第2前臼齿处横切）UseofRingSizer（环尺的使用）进行性萎缩性鼻炎—评估ARadicator®（克伟®

）–Efficacy（效力）ARadicatorisapatentedformulationofkilledbacteria,toxoid,andadjuvant:BordetellabronchisepticaPasteurellamultocida,TypeD(Toxigenic)Pasteurellamultocida,TypeDToxoidAmphigen®(adjuvant)Administer2mLintramuscularlytoswineabout6weeksand2weekspriortotheirfirstfarrowingAdminister2mLintramuscularlytoswineatabout2weekspriortosubsequentfarrowings克伟®是一种含有灭活细菌、类毒素和佐剂的专利配方：-支气管败血波氏杆菌-D型产毒素多杀性巴氏杆菌-D型多杀性巴氏杆菌类毒素-爱菲金（Amphigen）佐剂在第一次产仔前约6周和2周各肌肉注射接种2毫升；以后产仔前约2周肌肉注射接种2毫升ARadicator®（克伟®）–Efficacy（效力）ARadicatorprovidedprotectiontosucklingpigschallengedexperimentallyProtectedagainstchallengewithBordetellabronchisepticaProtectedagainstchallengewithtoxigenicPasteurellamultocida,TypeDProtectedagainstcombinedchallengewithBordetellabronchisepticaandPasteurellamultocida,TypeD克伟®可对实验攻毒的哺乳仔猪提供保护

#保护支气管败血波氏杆菌的攻击#保护D型产毒素多杀性巴氏杆菌的攻击#保护支气管败血波氏杆菌和D型产毒素多杀性巴氏杆菌的联合攻击ARadicator®（克伟®）–Efficacy（效力）EvaluationofResponseofSowstosingleboosterrevaccinationDurationofMaternalAntibodyinpigsCorrelationsofsowandpigletantibodyCorrelationsofantibodyresponsesandprotectionfromchallenge

评估母猪对再次免疫接种的反应仔猪母源抗体的持续时间母猪及仔猪抗体的相关性抗体应答与攻毒保护的相关性ARadicator®–Efficacy:BordetellabronchisepticaStudy186–Results（试验186–结果）SowSerologicResponses(GeometricMeanTiters)（母猪血清学反应，几何平均效价）Thevaccinateshadsignificantly(P≤0.05)highertitersthancontrols疫苗组的效价显著(P≤0.05)高于对照组的效价克伟–效力：支气管败血波氏杆菌Pre1stVacc.Pre2ndVacc.Farrowing,Group1Farrowing,

Group2Placebo对照1:641:591:741:48ARadicator1X克伟1个剂量1:641:9391:38171:7313ARadicator½X克伟1/2个剂量1:491:7241:24651:4756ARadicator®–Efficacy:BordetellabronchisepticaStudy186-Results（试验186–结果）PigletSerologicTiters(GeometricMeanTiters)（仔猪血清学反应，几何平均效价）Titersofpigssucklingvaccinatedgiltsweresignificantly(P≤0.05)higherthantitersofcontrols疫苗组仔猪的效价显著(P≤0.05)高于对照组的效价克伟–效力：支气管败血波氏杆菌No.ofPigsPre-ChallengePlacebo8litters

37pigs1:32ARadicator1X8litters

36pigs1:3694ARadicator½X8litters

35pigs1:2718ARadicator®–Efficacy:BordetellabronchisepticaStudy186-Results（试验186–结果）PigletTurbinateAtrophyScores(RingSizer)仔猪鼻甲骨萎缩评分（环尺法）Evaluated3weeksafterchallenge(4–6weeksold)

攻毒后3周进行评估（4-6周龄）ShownasLeastSquaresMean±StandardError（以最小平方值和标准差表示）克伟–效力：支气管败血波氏杆菌NumberofPigsRingSizerScoresRangePlacebo8litters–37piglets10.64to22ARadicator1X8litters–36piglets8.3*3to13ARadicator½X8litters–36piglets8.3*3to21*F-testfortreatmenteffectswasnotsignificant(F=0.1283)F-检验免疫效果不显著(F=0.1283)ARadicator®–Efficacy:BordetellabronchisepticaStudy186–CONCLUSIONS（结论）GoodSerologicResponsetoB.bronchisepticavaccination对支气管败血波氏杆菌的良好免疫反应Mildchallengeincontrols对照组出现轻度病变Trendtowardprotectionfromchallengeinvaccinates免疫组显现对攻毒的保护Goodtransferofantibodyfromvaccinatedsowtopiglet(goodcorrelationoftitervalues)免疫母猪抗体成功转移给仔猪（抗体滴度的高度相关）克伟–效力：支气管败血波氏杆菌ARadicator®–Efficacy:PasteurellamultocidaStudy178-Methods

Sows(Gilts)Pregnantgiltswithlowanti-toxintiterstoP.multocida8each(24total)placeboexperimentalARadicatorvaccine(minimumpotency)2doses,2mLeach,intramuscular6weeksand2weeksbeforefarrowingEarly-weanedPigs–AllowedtosuckleWeanedatabout1weekofageChallengedat2to4weeksofageTurbinateatrophyevaluatedat3weekslater克伟–效力：多杀性巴氏杆菌试验178–方法母猪（小母猪）

#低多杀性巴氏杆菌血清抗毒素效价的怀孕母猪#每组8头（共16头）-盐水对照-实验克伟疫苗#2个剂量，1个剂量2毫升，肌肉注射#产仔前6周和2周接种早期断奶仔猪–#自由吃奶#1周龄断奶#在2-4周龄攻毒#在攻毒后3周评估鼻甲骨萎缩程度ARadicator®–Efficacy:PasteurellamultocidaStudy178-Methods

ChallengeMethodChallengedintranasallywith1%aceticacid(1mLpernostrilontwoconsecutivedaysChallengedintranasallywithToxigenicPasteurellamultocida,TypeD,strain4677,onsubsequentthreeconsecutivedaysConcentration/mL:8.9X109;1.1X1010;1.0X1010克伟–效力：多杀性巴氏杆菌试验178–方法攻毒方法#用1%乙酸鼻内接种，每个鼻孔1毫升，连续2天#用D型产毒素多杀性巴氏杆菌（4677毒株）鼻内攻毒，连续3天击#细菌浓度（每毫升）为：8.9X109;1.1X1010;1.0X1010

ARadicator®–Efficacy:PasteurellamultocidaStudy178-Methods

SerologicResponseP.multocidaantitoxin(toxinneutralization)titersSowSerumPigletSerumTurbinateatrophyinpigletsRingSizerMethod4measurementspersnoutMinimumscore=8persnout克伟–效力：多杀性巴氏杆菌试验178–方法血清学反应#多杀性巴氏杆菌抗毒素（毒素中和试验）效价#母猪血清

#仔猪血清仔猪鼻甲骨萎缩程度#环尺法#每个鼻横切面测量4个值#每个鼻子的最小评分值=8ARadicator®–Efficacy:PasteurellamultocidaStudy178-Results（试验178–结果）SowSerologicResponses(GeometricMeanTiters)（母猪血清学反应，几何平均效价）*Thevaccinateshadsignificantly(P≤0.0001)highertitersthancontrols疫苗组的效价显著(P≤0.0001)高于对照组的效价Pre1stVacc.首免前Pre2ndVacc.二免前Farrowing,Group1分娩，组1Farrowing,

Group2分娩，组2Placebo对照1.31.31.31.2ARadicator克伟1.31.357.3*20.8*克伟–效力：多杀性巴氏杆菌ARadicator®–Efficacy:PasteurellamultocidaStudy178-Results（试验178–结果）PigletSerologicTiters(GeometricMeanTiters)（仔猪血清学反应，几何平均效价）*Titersofpigssucklingvaccinatedgiltsweresignificantly(P≤0.0019)higherthantitersofcontrols疫苗组仔猪的效价显著(P≤0.0019)高于对照组的效价No.ofPigs猪的数量Pre-Challenge攻毒前Placebo对照33pigs（头）1.3ARadicator克伟32pigs（头）8.4克伟–效力：多杀性巴氏杆菌ARadicator®–Efficacy:PasteurellamultocidaStudy178-Results（试验178–结果）PigletTurbinateAtrophyScores（仔猪鼻甲骨萎缩评分）Evaluated3weeksafterchallenge(5-7weeksold)攻毒后3周进行评估（5-7周龄）ShownasLeastSquaresMeanandRange以最小均方值和范围表示NumberofPigs猪的数量RingSizerScores环尺法分值

Range范围Placebo对照28pigs（头）16.69to31ARadicator克伟27pigs（头）8.6*4to16*Protectionfromturbinateatrophywassignificant(P=0.0001)inpigletssucklingvaccinateddams,comparedtocontrols.对疫苗组仔猪鼻甲骨萎缩的保护与对照组有显著差异(P=0.0001)克伟–效力：多杀性巴氏杆菌ARadicator®–Efficacy:PasteurellamultocidaStudy178–CONCLUSIONS结论GoodSerologicResponsetoP.multocidavaccination对多杀性巴氏杆菌/类毒素良好的免疫反应Sufficient,moderatechallengeincontrols对照组出现足够的、中度病变Excellentprotectioninpigletsweanedfromvaccinatedsows对免疫母猪所产仔猪的优良保护Goodtransferofantibodyfromvaccinatedsowtopiglet(goodcorrelationoftitervalues)免疫母猪抗体成功转移给仔猪（抗体滴度的高度相关）ARadicator®–Efficacy:CombinedChallengeStudy152–PhaseII-Methods

Sows(Gilts)Pregnantgiltswithlowanti-toxintiterstoP.multocidaandlowagglutinationtiterstoB.bronchiseptica7each(14total)placeboexperimentalARadicatorvaccine(minimumpotency)2doses,2mLeach,intramuscular6weeksand2weeksbeforefarrowingSucklingPigsWeanedandchallengedatabout4weeksofageTurbinateatrophyevaluated3weekslater克伟–效力：联合攻毒试验152–第II阶段-方法母猪（小母猪）

#低多杀性巴氏杆菌血清抗毒素效价和低支气管败血波氏杆菌凝集效价的怀孕母猪#每组7头（共14头）-盐水对照-实验克伟疫苗#2个剂量，1个剂量2毫升，肌肉注射#产仔前6周和2周接种哺乳仔猪#断奶，约在4周龄攻毒#在攻毒后3周评估鼻甲骨萎缩程度ARadicator®–Efficacy:CombinedChallengeStudy152–PhaseII-Methods

ChallengeMethodChallengedintranasallywith2.9X109

B.bronchiseptica,StrainB133onfirstdayofchallengeChallengedintranasallywithToxigenicPasteurellamultocida,TypeD,onfourconsecutivedays,fromDay3to6afterB.bronchisepticachallengeConcentration/mL:1.8X1010;2.4X1010;1.9X1010;and1.3X1010

克伟–效力：联合攻毒试验152–第II阶段-方法攻毒方法

#在攻毒的第一天用2.9X109支气管败血波氏杆菌（B133菌株）鼻内攻毒

#支气管败血波氏杆菌攻毒后3-6天，用D型产毒素多杀性巴氏杆菌鼻内攻毒，连续4天

#细菌浓度（每毫升）为：1.8X1010;2.4X1010;1.9X1010；1.3X1010

ARadicator®–Efficacy:CombinedChallengeStudy152–PhaseII-Methods

SerologicResponseP.multocidaantitoxin(toxinneutralization)titersSowSerumSowColostrumPigletSerumB.bronchisepticaagglutinationtitersSowSerumPigletSerum克伟–效力：联合攻毒试验152–第II阶段-方法血清学反应#多杀性巴氏杆菌抗毒素（毒素中和试验）效价-母猪血清-初乳-仔猪血清#支气管败血波氏杆菌凝集效价-母猪血清

-仔猪血清

ARadicator®–Efficacy:CombinedChallengeStudy152–PhaseII-Methods

TurbinateatrophyinpigletsRingSizerMethod4measurementspersnoutMinimumscore=8persnout克伟–效力：联合攻毒试验152–第II阶段-方法仔猪鼻甲骨萎缩程度#环尺法#每个鼻横切面测量4个值#每个鼻子的最小评分值=8ARadicator®–Efficacy:CombinedChallengeStudy152–PhaseII–Results（试验152–第II阶段–结果）P.multocidaantitoxintiters（多杀性巴氏杆菌抗毒素效价）*Thevaccinatedsowshadsignificantly(P=0.04)higherserumtitersthancontrols疫苗组母猪的效价显著(P=0.04)高于对照组的效价SowSerum,

Post-Farrowing母猪血清，产后Colostrum,

Farrowing初乳，分娩PigSerum(pooled),

Pre-challenge仔猪血清，攻毒前Placebo对照1.31.251.25ARadicator克伟2.7639.113.05克伟–效力：联合攻毒ARadicator®–Efficacy:CombinedChallengeStudy152–PhaseII–Results结果B.bronchisepticaagglutination母猪支气管败血波氏杆菌凝集效价SowSerologicResponses(GeometricMeanTiters)

（母猪血清学反应，几何平均效价）Thevaccinateshadsignificantly(P=0.009)highertitersthancontrols疫苗组的效价显著(P=0.009)高于对照组的效价Pre1stVacc.Pre2ndVacc.AfterFarrowingPlacebo1:5921:11851:6146ARadicator1:5431:103361:13404克伟–效力：联合攻毒ARadicator®–Efficacy:CombinedChallengeStudy152–PhaseII–Results结果B.bronchisepticaagglutination仔猪支气管败血波氏杆菌凝集效价PigletSerologicTiters(GeometricMeanTiters)（仔猪血清学反应，几何平均效价）Thedifferencebetweenpigletssucklingvaccinatedsowsversuscontrolswasnotsignificant(P>0.05)免疫组和对照组的差异不显著(P>0.05)Pre-ChallengePlacebo1:1777ARadicator1:7247克伟–效力：联合攻毒ARadicator®–Efficacy:CombinedChallengeStudy152–PhaseIIResults（试验152–第II阶段–结果）PigletTurbinateAtrophyScores（仔猪鼻甲骨萎缩评分）Evaluated3weeksafterlastchallenge(8weeksold)最后一次攻毒后3周进行评估（8周龄）ShownasLeastSquaresMean±StandardError以最小均方值±标准差表示NumberofPigs猪的数量RingSizerScores环尺法分值

Placebo对照28pigs（头）29.83±1.36ARadicator克伟33pigs（头）9.84±1.28**Protectionfromturbinateatrophywassignificant(P=0.0001)inpigletssucklingvaccinateddams,comparedtocontrols.对疫苗组仔猪鼻甲骨萎缩的保护与对照组有显著差异(P=0.0001)克伟–效力：联合攻毒ARadicator®–Efficacy:CombinedChallengeStudy152–PhaseII-additionalfindings其他发现Ahighincidenceofpneumoniawasobservedamongcontrols(duetosevereintranasalchallenge)由于高强度鼻内攻毒，对照组出现大量肺炎IncidenceofpneumoniawasreducedintheARadicatorvaccinatedpigs克伟免疫组肺炎发生减少LowerdosesofARadicatorweretriedandwerenotprotectiveagainstchallenge对低剂量克伟进行了试验，但不能有效保护攻毒克伟–效力：联合攻毒ARadicator®–Efficacy:CombinedChallengeStudy152–PhaseII-Results结果Correlations(Pearson)–B.bronchiseptica

component支气管败血波氏杆菌凝集效价相关性ComparisonrPSowSerumvs.

PigletSerum母猪血清与仔猪血清0.24470.0087SowSerumvs.

PigletRingSizerScore母猪血清与仔猪环尺法计分-0.29320.0015PigletSerumvs.

PigletRingSizerScore仔猪血清与仔猪环尺法计分-0.14210.1316克伟–效力：联合攻毒ARadicator®–Efficacy:CombinedChallengeStudy152–PhaseIICONCLUSIONS结论Thecombinedchallengewassevere(meanatrophyscoresof29)incontrols联合攻毒对对照组影响严重（平均萎缩计分为29）SowsvaccinatedwithARadicatorprotecttheirsucklingpigsfromseverechallengeat4weeksofage母猪接种克伟可保护在4周龄受到严重攻击的仔猪ExcellentSerologicresponsestoantigensinthevaccine对疫苗中抗原产生优异的血清学应答Goodtransferofantibodyfromvaccinatedsowtopiglet(goodcorrelationoftitervalues)免疫母猪抗体成功转移给仔猪（抗体滴度的高度相关）克伟–效力：联合攻毒ARadicator®–Efficacy:MaternalAntibodyStudy215–MaternalAntibodyinPigletsPigletsfromsowsvaccinatedaspartof210studySerumcollectedperiodicallyfromweaning(1weekofage)forabout12weeks(pigs13weeksofage)EvaluatedB.bronchisepticaagglutinationtitersandP.multocidaantitoxin(ELISA)titers克伟®–效力：母源抗体试验215–仔猪的母源抗体为试验210母猪所产的仔猪从断奶（1周龄）仔猪定期采血，持续12周（13周龄）测定支气管败血波氏杆菌凝集效价和多杀性巴氏杆菌抗毒素效价（ELISA）ARadicator®–Efficacy:MaternalAntibodyStudy215–MaternalAntibodyinPiglets–Results试验215–仔猪的母源抗体–结果Bordetellabronchisepticaagglutinationtiters(GeometricMeans)支气管败血波氏杆菌凝集效价（几何平均值）Dayofstudy试验天数

Day–1试验前1天Day7第7天Day28第28天Day41