琼浆细菌的突变试验结告v150cnen

被验物质Test猴头菇琼浆（韩国销售品）HericiumerinaceumMushroomLiquidproductsSoldinModulationofthesample试验中使用菌株Strainsusedinthe(独)200947日Productevaluationtechniquesbaselinebody.(财)发酵2002年1月17日/FermentationResearchInstitute.DMSO0.07ml.S9制造元（株）200992日S9Manufacturingstrain),OrientalYeastIndustryDate:September2nd2009S9mix的构成Thecompositionof成分S9mix1ml中的量/IntheamountofingredientsS9mix1ml成分S9mix1ml中的成分TheingredientcompositionS9mix1mlS90.1mlNADPH4mmol.MgCl28mmolNADH4葡萄糖-6-5mmolGlucose6phosphate5葡萄糖-6-Glucose6phosphateThenameandmanufacturerofNutrientbrothpre-conditionsforMakethehighestconcentrationoftheabovesuspensionliquid,Commonratio2to5stagesofdilutesolutionasthesample.最小葡萄糖Minimal琼脂平板培养基（）/garmediumS9mix调制用的辅助因子使用的是东方酵母工业所生产的产品。NutrientBrothNo.2forAmesOxoid(2.5%)生产。S9mixmodulationcofactorsusedintheuseofthe(strain),fromproductsofOrientalYeastindustries.NutrientBrothNo.2byManufacturedforAmesOxoid(2.5%).TheuseofSalmonellatyphimuriumTA98SalmonellatyphimuriumTA100EscherichiacoliWP2/uvrA试验条件Test37Temperature3748—72Time48-72AF-2和光纯药株式会社/AF-2,和光纯药ChemicalCo.,.9AA公司/9AAPR.2AA和光纯药株式会社/和光纯药ChemicalCo.,.NaN3关东化学株式会社/NaN3KantoChemicalCo.,.备考参考备考试验责任人名称/Thenameof sin地址所在地：群马县市中大类町37-1健康福祉大学。试验日期：200910620091015日。前置培養法/Pre-culturemethod*注释Throughoutthetrial,confirmedthattheobjectdoesnotincreasebymorethannegativezone2timesthenumberofrecoverygroups(3timesofTA15