DNA试验-免疫细胞表面抗原分子CD家族对照表

免疫细胞表面抗原分子CD家族对照表(CD1-CD247)ExalphaBiologicalsproducesandsellsproductsforLifeScieneeResearch,includingmonoclonalandpolyclonalantibodies,foruseinimmunohistochemistry,westernblot,ELISAandFlowCytometry.Weofferover800productsand,throughcollaborations withotherscientistsaswellasourownin-houseR&Dprograms,Exalphacontinuestobringleadingedgeproductstomarket.OurfamilyofCDantibodiescombinesqualityandperformaneewithreasonablepricestomakeourCDantibodiesthebestvalueinthemarkettoday.OurfamilyofProliferationproductsoffereasyall-in-onekitsorstandaloneantibodiestoquantitateproliferating cells.Takealookatourwebsiteorcallusforadditionalinformation.Note: noinformationisavailableonCDw145,CD181,CD182,CD185-194,CD196-199,CD211,CD214-216,CD218-220, CD237BackgroundCD:clusterdesignationofmonoclonalantibodies(clustersofdifferentiation)Designatedatthe1stto7thWorkshopsonInternationalHumanLeukocyteDifferentiationAntigensThelastconferencewasin2000.Thenextconferenceisin2004.HLDAWorkshopsaretheprimarymechanismtocharacterizeleukocytesurfaceantigenicmoleculesandepies;erythroidantigensarenowincludedFor1stto6thworkshops,antibodiesweresubmittedtotheorganizinglaboratory,codedandsenttoparticipatinglaboratoriesfortestingagainst variouscelltypes.Forthe7thworkshop,aCDdesignationcouldbeestablishedforamoleculeifitsgenehasbeenclonedandatleastonespecificmonoclonalantibodyhadbeenstudiedintheWorkshopInterpretationshouldbebasedoncellulardistributionofstaining,proportionofpositivelystainedcells,stainingintensityandcutofflevels.CD1Familyofnon-polymorphicMHCclassI-likeglycoproteinsAlsomemberofimmunoglobulinsuperfamilyOnchromosome1q22-23(notMHClinked)Has5differentsubsets,allnoncovalentlyassociatedwith12kdbeta2microglobulinFunction: restrictTcellresponsestocertain antigens; maymediatethymicTcelldevelopmentPositivestaining(normal): corticalthymocytes(70%),activatedTcells,Langerhanscells,interdigitating dendriticcellsPositivestaining(disease): preTALLwithcorticalthymocytephenotype;LangerhanscellhistiocytosisNegativestaining: matureperipheralTcellsCD1aPositivestaining(normal): Dendriticcellsindermis/epidermisofbenigninflammatoryskindisordersPositivestaining(disease): Langerhanscellhistiocytosis(fairlyspecific),myeloidleukemias,someBcellmalignancies;dendriticcellsinmostperipheralcutaneousTcelllymphomas,AJCP2001;116:72Bcelllymphomas(?reflectsreplacementofreactivepatterncontainingdendriticcellswithaneoplasticpatternofBcells)Microimages: LangerhanscellhistiocytosisMicroimages(AJSPsubscribers): pulmonaryLangerhanscellhistiocytosisMicroimages(HumPathsubscribers): LangerhanscellhistiocytosisReferences: AJSP2001;25:630CD1bPositivestaining(disease): myeloidleukemiasandsomeBcellmalignanciesNegativestaining: normalBcellsCD1cPositivestaining(normal): subsetofnormalperipheralBcellscellmalignanciesNegativestaining:normalBcellsNegativestaining:normalBcellsCD1dPositivestaining(normal):thymus(lowlevels),bowelPositivestaining(normal):thymus(lowlevels),bowelCD1eCD2AkaErosettereceptor,LFA-2(leukocytefunctionantigen)Function: bindsCD58/LFA-3onantigen-presenting cells,andinducescostimulatorysignalsinTcellsAlsoregulatesTandNK-mediatedcytolysis,inhibitsapoptosisofactivatedperipheralTcells,mediatesTcellcytokineproduction,regulatesTcellanergyPositivestaining(normal): thymocytes(95%),matureperipheralTcells(almostall),NKcells(80-90%),thymicBcells(50%)Microimages: extranodalNK/Tcelllymphoma,nasaltypeCD2RCD2epiesrestrictedtoactivatedTcellsPositivestaining: activatedTcells,?NKcellsCD3AkaOKT3Function: complex(5chains)ofintegralmembraneglycoproteinsassembledasacomplex;haslongcylasmictailwithantigenrecognition activationmotif;complexisthendownregulatedAlsosubdividedintodelta,epsilon,gammasubtypesCylasmicexpressionatearlyTcelldifferentiation,thenmembranousexpressionMostspecificTcellantibodyPositivestaining(normal): thymocytes,peripheralTcells,NKcells;alsoPurkinjecellsofcerebellumPositivestaining(disease): 80%ofTcelllymphomaslymphomasMicroimages: CD3epsilon-testicularNK/Tcelllymphoma(figure3D)Microimages(AJSPsubscribers): achalasia, post-transplantlymphoproliferativediseaseinliverReferences: AJSP2001;25:1413CD4AkaOKT4,Thelper/inducerOnchromosome#12pNonpolymorphousglycoproteinsbelongingtoimmunoglobulinsuperfamilyServesasHIVreceptoronTcells(asdochemokinereceptorsCCR5andCXCR4),macrophages,brainCD4+TcellsarekilledbyHIVCoreceptorinMHCclassII-restrictedantigeninducedTcellactivationBindstononpolymorphicregionofclassImolecules;mayincreaseavidityofcell-cellinteractionsPositivestaining(normal): thymocytes(80-90%),matureTcells(65%,ThelperandCD4/CD8+thymocytes),macrophages,Langerhanscells,dendriticcells,granulocytesPositivestaining(disease): pityriasislichenoidesMicroimages: acutedemyelinatingdisease,extranodalNK/Tcelllymphoma,nasaltypeCD5BelongstoancientscavengerreceptorfamilyIsphysicallyandfunctionallycoupledwithTcellreceptor-zeta-CD3signaltransducercomplexCD5+Bcellsproduce“generalistantibodies”-polyreactivelowaffinity “natural”antibodiestoexogenousantigens(tetanustoxoid,lipopolysaccharide)aswellasautoreactiveantibodies(ssDNA,thyroglobulin,insulin)Note:sharksonlyhavepolyreactiveIgMNote:monoreactiveIgGisproducedby<0.1%ofcirculatingBcells,frompositiveselectionandsomaticpointmutationFirstlineofdefenseagainstantigens;havealowactivationthreshold;aretheonlylineofdefenseforthosewhocannotproducespecificantibodyProduceantibodiesusinggermline(nonmutated)configurationofgenesegments,usuallyIgMProductionelevatedinrheumatoidarthritis(27-52%ofcirculatingBcellsvs.20%normal)CD5mayserveasadualreceptor, givingeitherstimulatoryorinhibitorysignalsdependingbothonthecelltypeandthedevelopmentstagePositivestaining(normal): Bcellsof mantlezoneofspleenandlymphnodes;Bcellsinperitonealandpleuralcavities;almostallTcells;Infetus,mostBcellsinspleenandcordbloodareCD5positivePositivestaining(disease): BcellCLL/SLL,mantiecelllymphoma,mostTmalignancies,thymiccarcinomas(70%)Negativestaining: spindlecellthymomas,MALTlymphoma,follicularlymphomaMicroimages: extranodalNK/Tcelllymphoma,nasaltype,mantlecelllymphoma(figure3D)CD6AdhesionmoleculemediatingthebindingofdevelopingthymocyteswiththymicepithelialcellsMaybeinvolvedinautoimmunityandgraftvs.hostdisease(GVHD)AntibodiestoCD6areusedtodepleteTcellsfrombonemarrowtransplantstopreventGVHDPositivestaining(normal): lowlevelsonimmaturethymocytes,highlevelsonmaturethymocytesCD7MembraneglycoproteinandFcreceptorforIgMHomologoustoTCRgamma,IgkappaMembraneexpressionearlyduringTontogeny,beforeTCRrearrangement,persistsuntilterminalstagesofTcelldevelopmentLowerexpressioninmemoryTcellsvs.naiveTcellsPositivestaining(normal): matureperipheralTcells(85%),post-thymicTcells(majority),NKcells(majority),somemyeloidcellsPositivestaining(disease): TcellALL;AML(especiallyM4/M5),chronicmyelogenousleukemia,blastsintransientmyeloproliferativedisorderNegativeexpression: BcellALL,Sezarysyndrome,adultTcellleukemia/lymphomaMicroimages: extranodalNK/Tcelllymphoma,nasaltypeCD8AkaOKT8,Tcellsuppressor/cytotoxiccellsOnchromosome#2MHCCIassIrestrictedreceptor;bindstononpolymorphicregionofclassImolecules;mayincreaseavidityofcell-cellinteractionsAssociatedwithlymphoepithelioma-like carcinomaoflung(AJSP2002;26:715)Positivestaining(normal): Tcells(25-35%ofmatureperipheralTcells,mostcytotoxicTcells,CD4/CD8+thymocytes);NKcells(30%-whicharealsoCD3negative);corticalthymocytes(70-80%),epidermotrophiclymphocytesinmycosisfungoides(AJSP2002;26:450)Microimages: lymphoepithelioma-likecarcinomaofcervix-figure3,acutedemyelinatingdiseaseMicroimages(ModPathsubscribers): nodalcytotoxicTcelllymphomaReferenee: ModPath2002;15:1131CD9MaymediateplateletactivationandaggregationAntibodiesareusedtopurgebonemarrowpriortoperipheralstemcellbonemarrowtransplantViralco-receptorPositivestaining(normal): preBcells,Bcellsubset,Tcells,ma