湿热消毒灭菌法徐单单Wetheatdisinfectionandsterilizationmeth

湿热消毒灭菌法主讲老师：徐单单WetheatdisinfectionandsterilizationmethodGuangdongFoodandDrugVocationalCollege广东食品药品职业学院湿热消毒灭菌法01Wetheatdisinfectionandsterilizationmethod01湿热消毒灭菌法Wetheatdisinfectionandsterilizationmethod湿热消毒灭菌法Wetheatdisinfectionandsterilizationmethod煮沸消毒法Boilingdisinfectionmethod1流通蒸汽消毒法Circulatingsteamdisinfectionmethod2间歇蒸汽灭菌法Fractionalsterilization3巴氏消毒法Pasteurizationmethod4高压蒸汽灭菌法Sterilizationbypressuredsteam5煮沸消毒法Boilingdisinfectionmethod湿热消毒灭菌法Wetheatdisinfectionandsterilizationmethod100℃，5-10min消毒Disinfectat100℃for5-10minutes适用于饮水、餐具、刀、剪、小容量口服液和注射剂Suitablefordrinkingwater,tableware,knives,scissors,smallvolumeoralliquids,andinjections流通蒸汽消毒法Circulatingsteamdisinfectionmethod湿热消毒灭菌法Wetheatdisinfectionandsterilizationmethod

100℃，5-10min消毒Disinfectat100℃for5-10minutes餐具、口服液、管道、小容量口服液和注射剂Tableware,oralliquids,tubing,smallvolumeoralliquids,andinjections间歇蒸汽灭菌法Intermittentsteamsterilizationmethod湿热消毒灭菌法Wetheatdisinfectionandsterilizationmethod100℃连续三天每天蒸汽一次可灭菌Steamingonceadayforthreeconsecutivedaysat100℃cansterilize适用于管道或不耐高温的培养基、药品、营养液灭菌Suitableforsterilizationofpipelinesorculturemedia,drugs,andnutrientsolutionsthatarenotresistanttohightemperatures湿热消毒灭菌法Wetheatdisinfectionandsterilizationmethod63℃

30min

或

72℃15s消毒适用范围：牛奶、奶酪、果汁、酒类、糖浆、果脯、酱菜等食品消毒、注射用水储藏优点不破坏营养成分不影响风味缺点只消毒，不灭菌巴氏消毒法(低温消毒法)图1.巴氏消毒法的适用范围

Figure1.ScopeofApplicationofPasteurizationMethod高压蒸汽灭菌法High-pressuresteamsterilizationmethod湿热消毒灭菌法Wetheatdisinfectionandsterilizationmethod方法1：121.3℃，103.42KPa(1.05kg/cm2,15Ib/in2)15-30minMethod1:121.3℃,103.42KPa(1.05kg/cm2,15Ib/in2)15-30min方法2：115℃，68.95KPa(0.703kg/cm2,10Ib/in2)30minMethod2:At115℃,68.95KPa(0.703kg/cm2,10Ib/in2)for30min湿热消毒灭菌法Wetheatdisinfectionandsterilizationmethod适用范围：耐高温不怕湿的物品，微生物实验材料、药品生产、医疗用品

Scopeofapplication:Itemsthatareresistanttohightemperaturesandnot

afraidofmoisture，microbialexperimentalmaterials,drugproduction,

medicalsupplies优点：快速灭菌，湿热灭菌法中最好的方法Advantages:Rapidsterilization,thebestmethodamongmoistheatsterilizationmethods缺点：灭菌后物品潮湿Disadvantage:Itemsbecomedampaftersterilization

①

高压蒸汽灭菌法的具体适用范围①

Thespecificscopeofapplicationofhigh-pressuresteam

sterilizationmethod微生物实验，普通培养基、生理盐水、玻璃器皿、金属、陶瓷制品Microbialexperiment,ordinaryculturemedium,physiologicalsaline,glassware,metal,ceramicproducts药品生产，化学原料、植物原料、辅料、注射剂（如输液）、胶塞、容器、

工作服、制、储水设备、管道Drugproduction,chemicalrawmaterials,plantrawmaterials,excipients,injections(suchasinfusion),stoppers,containers,workclothes,waterstorageequipment,pipelines医疗用品，无菌器械、敷料、手术用具、手术衣等Medicalsupplies,sterileinstruments,dressings,surgicaltools,surgicalgowns,etc图2.高压蒸汽灭菌法的适用范围

Figure2.Scopeofapplicationofhigh-pressuresteamsterilizationmethod图3.高压蒸汽灭菌法的适用范围

Figure3.Scopeofapplicationofhigh-pressuresteamsterilizationmethod图4.高压蒸汽灭菌法的适用范围

Figure4.Scopeofapplicationofhigh-pressuresteamsterilizationmethod图5.手提式灭菌锅

Figure5.Portablesterilizationpot②高压蒸汽灭菌器类型:手提式、立式、卧式图6.立式灭菌锅

Figure6.Verticalsterilizationpot图7.卧式灭菌锅

Figure7.Horizontalsterilizationpot③高压蒸汽灭菌注意事项③Precautionsforhigh-pressuresteamsterilization

定期检查压力表是否灵敏，压力与温度是否对应；Regularlycheckwhetherthepressuregaugeissensitiveandwhetherthepressurecorrespondstothetemperature灭菌初期先排净锅内冷空气;Duringtheinitialstageofsterilization,thecoldairinsidethepotshouldbepurgedfirst灭菌时间计算：达到规定压力和温度开始计；Sterilizationtimecalculation:startcountingwhenthespecifiedpressureandtemperaturearereached

锅内水分要充足；Thewaterinthepotshouldbesufficient灭菌物品装量不宜太满；Theamountofsterilizeditemsshouldnotbetoofull灭菌结束需缓慢减压；Slowdecompressionisrequiredaftersterilizationiscompleted灭菌结束，趁热取出物品。Sterilizationiscomplete,takeouttheitemswhiletheyarestillhot小结：以上就是我们这次课的主要学习内容，主要讲述了湿热消毒灭菌法，重点讲述高压蒸汽灭菌法、适用范围、灭菌锅类型。Summary:Theaboveisthemaincontentofourlesson,which