1-3-Diphenylisobenzofuran-solution-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemE1,3-Diphenylisobenzofuran(solution)Cat.No.:HY-DY1053CASNo.:5471-63-6分⼦式:C₂₀H₁₄O分⼦量:270.33作⽤靶点:FluorescentDye;ReactiveOxygenSpecies(ROS)作⽤通路:Others;Immunology/Inflammation;MetabolicEnzyme/Protease;NF-κB储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY⽣物活性1,3-Diphenylisobenzofuran(DPBF)(solution)作为⼀种选择性探针，⽤于检测和定量测定含有不同活性氮和氧物种(RNOS)样品中的过氧化氢。DPBF⼀种荧光探针，近20年来，⼈们认为它与⼀些活性氧(如单重态氧和羟、烷氧或烷过氧⾃由)发⽣⾼度特异的反应[1]。溶剂及浓度：DMSO：10mM体外研究GeneralProtocolPreparationofDPBFworkingsolution1.1PreparationofthestocksolutionDissolve10mgofDPBFin3.6992mLofDMSOtoobtain10mMofDPBF.Note:Itisrecommendedtostorethestocksolutionat-20°C,-80°Cawayfromlightandavoidrepetitivefreeze-thawcycles.1.2PreparationofDPBFworkingsolutionDilutethestocksolutioninserum-freecellculturemediumorPBS.Thecorrespondingstocksolutioncanbedilutedaccordingtotheactualsituation.NotethatifthesolventisDMSO,thecytotoxicityofDMSOmustbeconsidered,andasolventcontrolshouldbeprepared;ifthesolventispurewater,theworkingsolutionneedstobefilteredandsterilizedbeforeaddingcells.Note:PleaseadjusttheconcentrationofDPBFworkingsolutionaccordingtotheactualsituation.Cellstaining2.1Forsuspensioncells:Centrifugeat1000gat4°Cfor3-5minutesandthendiscardthesupernatant.WashtwicewithPBS,5minuteseachtime.1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEForadherentcells:Discardthecellculturemedium,andaddtrypsintodissociatecellstomakeasingle-cellsuspension.Centrifugeat1000gat4°Cfor3-5minutesandthendiscardthesupernatant.WashtwicewithPBS,5minuteseachtime.2.2Add1mLofDPBFworkingsolution,andthenincubateatroomtemperaturefor30minutes.2.3Centrifugeat400gat4°Cfor3-4minutesandthendiscardthesupernatant.2.4WashtwicewithPBS,5minuteseachtime.2.5Resuspendcellswithserum-freecellculturemediumorPBS,andthendetectbyfluorescencemicroscopeorflowcytometer.Precautions1.Itisrecommendedtostorethestocksolutionat-20°Cor-80°Cawayfromlightandavoidrepetitivefreeze-thawcycles.2.PleaseadjusttheconcentrationofDPBFworkingsolutionaccordingtotheactualsituation.3.ThisproductisforR&Duseonly,notfordrug,household,orotheruses.4.Foryoursafetyandhealth,pleasewearalabcoatanddisposableglovestooperate.REFERENCES[1].WeiliFan,etal.Calciumcarbonate-methylenebluenanohybridsforphotodynamictherapyandultrasoundimaging.SciChinaLifeSci.2018Apr;61(4):483-491.[2].P.Carloni,etal.Ontheuseof1,3-diphenylisobenzofuran(DPBF).Reactionswithcarbonandoxygencenteredradicalsinmodelandnaturalsystems.ResearchonChemicalIntermediatesvolume19,pages395-405(1993).[3].BaiX,etal.HKOH-1:AHighlySensitiveandSelectiveFluorescentProbeforDetectingEndogenousHydroxylRadicalsinLivingCells.AngewChemIntEdEngl.2017Oct9;56(42):12873-12877.McePdfHeightCaution:Producthasnotbeenfullyvalid