荧光假单胞菌群体感应缺失突变株的构建及表型研究

[28]，二者关系仍然不明确。在食品加工及贮藏过程中，细菌往往面临着多种胁迫条件，包括氧化剂、乙醇、高温、低温、酸碱、高盐以及食品保鲜剂等，本研究只做了一个过氧化氢的胁迫条件，结果可重复性不佳，原因可能是由于菌株的生长状态的影响及实验环境、温度的影响，本实验及更多的胁迫条件需要后续进一步实验研究。综上所述，本实验成功构建了荧光假单胞菌群体感应基因缺失突变株，研究发现rhlI与lasI基因的缺失影响了荧光假单胞菌的指数生长，并影响其生物被膜产生、泳动能力、对过氧化氢的耐受性。rhlI与lasI基因对荧光假单胞菌生物表型的影响表明这两个基因在细菌致腐过程中发挥重要调控作用，本研究为后续进一步研究群体感应基因调控细菌致腐机制和探索食品保鲜、存储技术奠定理论基础。

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