BODIPY-493-503-solution-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEBODIPY493/503(solution)Cat.No.:HY-DY1004CASNo.:121207-31-6分⼦式:C₁₄H₁₇BF₂N₂分⼦量:262.11作⽤靶点:FluorescentDye作⽤通路:Others储存⽅式:-20°C,protectfromlight.Avoidrepeatedfreezingandthawing*Insolvent:-80°C,6months;-20°C,1month(protectfrom

light.Avoidrepeatedfreezingandthawing)BIOLOGICALACTIVITY⽣物活性BODIPY493/503(solution)⼀种BODIPY染料。BODIPY染料⼀种具有强紫外吸收能⼒的⼩分⼦染料，其荧光峰⽐较尖锐，量⼦产率⾼。其对环境的极性和pH值相对不敏感，因此，在不同⽣理条件下相对稳定。由于其结构的⾮对称性，BODIPY具有多种衍⽣结构产物。BODIPY脂滴类染料可以很好的穿过细胞膜进⼊细胞内部，通过在细胞内的中性脂类上定位以进⾏特异性染⾊，因此，该染料可⽤于标记活细胞和定细胞。最⼤激发/发射波长：493/503nm。溶剂及浓度：DMSO：5mM体外研究GeneralProtocol1.PreparationofBODIPY493/503workingsolutionThecorrespondingstocksolutioncanbedilutedaccordingtotheactualsituation.NotethatifthesolventisDMSO,thecytotoxicityofDMSOmustbeconsidered,andasolventcontrolshouldbeprepared;ifthesolventispurewater,theworkingsolutionneedstobefilteredandsterilizedbeforeaddingcells.2.Cellstaining2.1Suspensioncells(6-wellplate)a.Centrifugeat1000gat4°Cfor3-5minandthendiscardthesupernatant.WashtwicewithPBS,5mineachtime.Thecelldensityis1×106/mL.b.Add1mLofworkingsolution,andthenincubateatroomtemperaturefor5-30min.c.Centrifugeat400gat4°Cfor3-4minandthendiscardthesupernatant.d.WashtwicewithPBS,5mineachtime.e.Resuspendcellswithserum-freecellculturemediumorPBS.Observationbyfluorescencemicroscopyorflowcytometry.2.2Adherentcells1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEa.Cultureadherentcellsonsterilecoverslips.b.Removethecoverslipfromthemediumandaspirateexcessmedium.c.Add100μLofworkingsolution,gentlyshakeittocompletelycoverthecells,andthenincubateatroomtemperaturefor5-30min.d.Washtwicewithmedium,5mineachtime.Observationbyfluorescencemicroscopyorflowcytometry.StorageStoreinseparatecontainerstoavoidrepeatedfreeze-thawcycles.-20°C,1year;Protectfromlight.Precautions1.PleaseadjusttheconcentrationofBODIPY493/503workingsolutionaccordingtotheactualsituation.2.Experimentssuggestapositivecontrol,incubatethecontrolgroupcellswith30μMoleicacidfor8handthenperformsubsequentexperiments.3.ThisproductisonlyforR&Duse,notfordrug,household,orothers.4.Foryoursafetyandhealth,pleasewearalabcoatanddisposableglovestooperate.REFERENCES[1].BoQiu,etal.BODIPY493/503StainingofNeutralLipidDropletsforMicroscopyandQuantificationbyFlowCytometry.BioProtoc.2016Sep5;6(17):e1912.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplicat