BODIPY-Cholesterol-solution-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEBODIPY-Cholesterol(solution)Cat.No.:HY-DY1029CASNo.:878557-19-8分⼦式:C₃₆H₅₁BF₂N₂O分⼦量:576.62作⽤靶点:FluorescentDye作⽤通路:Others储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY⽣物活性BODIPY-cholesterol(solution)⼀种带有荧光BODIPY团的亲脂性和细胞通透性胆醇类似物。BODIPY-cholesterol可⽤于监测细胞内醇摄取和细胞器间醇通量。(激发/发射波长:505/515nm)。溶剂及浓度：DMSO：1mM体外研究1.Solutionpreparation[1]1.1StocksolutionStorage:Afteraliquoting,storeat-20°Cor-80°Cinthedarkandavoidrepeatedfreezingandthawing.1.2PreparationofworkingsolutionDilutewithPBS(orotherbuffer).Thecorrespondingstocksolutioncanbedilutedaccordingtotheactualsituation.NotethatifthesolventisDMSO,thecytotoxicityofDMSOmustbeconsidered,andasolventcontrolshouldbeprepared;ifthesolventispurewater,theworkingsolutionneedstobefilteredandsterilizedbeforeaddingcells.Note:Theworkingsolutionshouldbepreparedandusedimmediately,andprotectedfromlight.2.Cellstaining2.1WashcellswithPBS.2.2LabelthecellsuspensionwithBODIPY-Cholesterol(finalconcentration0.5-5μM),mixwellandincubateat37°Cfor10-20minutes.2.3Flowcytometricdetection(Ex/Em=510/590nm).REFERENCES1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE[1].DanielWüstner,etal.PotentialofBODIPY-cholesterolforanalysisofcholesteroltransportanddiffusioninlivingcells.ChemPhysLipids.2016Jan;194:12-28.[2].MaaritHölttä-Vuori,etal.BODIPY-cholesterol:anewtooltovisualizesteroltraffickinginlivingcellsandorganiss.Traffic.2008Nov;9(11):1839-49.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-820-3792;02