氧疗改善小鼠运动疲劳后睡眠障碍的作用研究

[25]。图8氧疗对运动疲劳后纹状体多巴胺释放的影响(A)植入纹状体碳纤维电极示意图及其工作原理。(B)经化学处理的小鼠脑组织中刺激多巴胺(DA)释放的代表性快速扫描循环伏安法(FSCV)彩色图。I-t曲线和循环伏安图为虚线部分。(C)氧疗后运动疲劳后纹状体DA平均释放量结论本研究探讨了运动性疲劳对睡眠和行为的影响及氧疗的干预效果，发现运动疲劳会导致脑电图中以α波为主，伴随β波及少量低幅慢波的增加，这种现象与疲劳引发的睡眠障碍有关；而氧疗干预显著延长了睡眠时间。此外，行为学实验表明，疲劳导致小鼠运动活力下降，但氧疗可逐步恢复其运动活力。通过快速扫描循环伏安法和碳纤维微电极，首次以电化学方法验证氧疗能够增强纹状体中多巴胺的刺激释放，揭示了多巴胺信号在运动性疲劳相关睡眠障碍中的重要作用。本研究为氧疗在疲劳干预中的应用提供了科学依据，并从神经和化学层面丰富了对运动性疲劳的认识。参考文献Kim,H.;Jung,H.R.;Kim,J.B.;Kim,D.-J.AutonomicDysfunctioninSleepDisorders:FromNeurobiologicalBasistoPotentialTherapeuticApproaches.JClinNeurol2022,18(2),140.Shah,N.M.;Bennett,C.;Hassan,H.;Kaltsakas,G.SleepDisordersandExercise:AMini-Review.JThoracDis2023,15(10),5863–5872.Hobson,J.A.SleepafterExercise.Science1968,162(3861),1503–1505.Wu,M.;Zhang,X.;Feng,S.;Freda,S.N.;Kumari,P.;Dumrongprechachan,V.;Kozorovitskiy,Y.DopaminePathwaysMediatingAffectiveStateTransitionsafterSleepLoss.Neuron2024,112(1),141-154.Dong,H.;Chen,Z.-K.;Guo,H.;Yuan,X.-S.;Liu,C.-W.;Qu,W.-M.;Huang,Z.-L.StriatalNeuronsExpressingDopamineD1ReceptorPromoteWakefulnessinMice.CurrentBiology2022,32(3),600-613.Yang,X.;Xue,Y.;Liu,R.;Zhao,X.;Li,K.;Wang,J.;Hou,L.DopamineReleaseImpairmentsAccompanyMovementVigorDeficiencyinanExercise-InducedFatigueMouseModel.ACSChem.Neurosci.2023,14(13),2443–2449.Courtin,J.;Bitterman,Y.;Müller,S.;Hinz,J.;Hagihara,K.M.;Müller,C.;Lüthi,A.ANeuronalMechanismforMotivationalControlofBehavior.Science2022,375(6576),Kujawski,S.;Słomko,J.;Morten,K.;Murovska,M.;Buszko,K.;Newton,J.;Zalewski,P.AutonomicandCognitiveFunctionResponsetoNormobaricHyperoxiaExposureinHealthySubjects.PreliminaryStudy.Medicina2020,56(4).Burbulla,L.F.;Song,P.;Mazzulli,J.R.;Zampese,E.;Wong,Y.C.;Jeon,S.;Santos,D.P.;Blanz,J.;Obermaier,C.D.;Strojny,C.;Savas,J.N.;Kiskinis,E.;Zhuang,X.;Krüger,R.;Surmeier,D.J.;Krainc,D.DopamineOxidationMediatesMitochondrialandLysosomalDysfunctioninParkinson’sDisease.Science2017,357(6357),1255–1261.Liu,W.;Liu,K.;Tao,H.;Chen,C.;Zhang,J.H.;Sun,X.HyperoxiaPreconditioning:ThenextFrontierinNeurology?NeurologicalResearch2012,34(5),415–421.Gottfried,I.;Schottlender,N.;Ashery,U.HyperbaricOxygenTreatment—FromMechanismstoCognitiveImprovement.Biomolecules2021,11(10),1520.Leighton,T.;VanHorne,E.;Parsons,D.OxygenStraighttotheBrain:AnOverviewofHyperbaricOxygenTherapyforaVarietyofBrainMorbidities.CurrSportsMedRep2024,23(4),130–136.Banou,E.HyperbaricOxygenTherapyEffecton“KinesiaParadoxa”BrainCircuits.InGeNeDis2020;Vlamos,P.,Ed.;AdvancesinExperimentalMedicineandBiology;SpringerInternationalPublishing:Cham,2021,139–146.Alashram,A.R.;Padua,E.;Romagnoli,C.;Annino,G.HyperbaricOxygenTherapyforCognitiveImpairmentsinPatientswithTraumaticBrainInjury:ASystematicReview.AppliedNeuropsychology:Adult2023,30(5),602–613.Scammell,T.E.;Arrigoni,E.;Lipton,J.O.NeuralCircuitryofWakefulnessandSleep.Neuron2017,93(4),747–765.Morawska,M.M.;Moreira,C.G.;Ginde,V.R.;Valko,P.O.;Weiss,T.;Büchele,F.;Imbach,L.L.;Masneuf,S.;Kollarik,S.;Prymaczok,N.;Gerez,J.A.;Riek,R.;Baumann,C.R.;Noain,D.Slow-WaveSleepAffectsSynucleinopathyandRegulatesProteostaticProcessesinMouseModelsofParkinson’sDisease.Sci.Transl.Med.2021,13(623),eabe7099.Lendner,J.D.;Niethard,N.;Mander,B.A.;VanSchalkwijk,F.J.;Schuh-Hofer,S.;Schmidt,H.;Knight,R.T.;Born,J.;Walker,M.P.;Lin,J.J.;Helfrich,R.F.HumanREMSleepRecalibratesNeuralActivityinSupportofMemoryFormation.Sci.Adv.2023,9(34),eadj1895.Tuan,L.-H.;Yeh,J.-W.;Lee,L.J.-H.;Lee,L.-J.SleepDeprivationInducesDopamineSystemMaladaptationandEscalatedCorticotrophin-ReleasingFactorSignalinginAdolescentMice.MolNeurobiol2023,60(6),3190–3209.Costa,A.E.A.;Silva,J.L.V.;Simões,M.J.MorphofunctionalAlterationsoftheNonpregnantMurineUterusinResponsetoIntenseandExhaustiveExerciseAreNotRelatedtoOxidativeStress.JournalofAppliedPhysiology2014,116(6),604–610.Hasegawa,E.;Miyasaka,A.;Sakurai,K.;Cherasse,Y.;Li,Y.;Sakurai,T.RapidEyeMovementSleepIsInitiatedbyBasolateralAmygdalaDopamineSignalinginMice.Science2022,375(6584),994–1000.Rodeberg,N.T.;Sandberg,S.G.;Johnson,J.A.;Phillips,P.E.M.;Wightman,R.M.Hitchhiker’sGuidetoVoltammetry:AcuteandChronicElectrodesforinVivoFast-ScanCyclicVoltammetry.ACSChem.Neurosci.2017,8(2),221–234.Xue,Y.;Ji,W.;Jiang,Y.;Yu,P.;Mao,L.DeepLearningforVoltammetricSensinginaLivingAnimalBrain.AngewChemIntEd2021,60(44),23777–23783.Ishihara,A.MildHyperbaricOxygen:MechanismsandEffects.JPhysiolSci2019,69(4),573–580.Matsumoto,A.;Okiura,T.;Morimatsu,F.;Ohira,Y.;Ishihara,A.EffectsofHyperbaricExp