HADA-hydrochloride-solution-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEHADAhydrochloride(solution)Cat.No.:HY-DY1044CASNo.:2253733-10-5分⼦式:C₁₃H₁₃ClN₂O₆分⼦量:328.71作⽤靶点:Bacterial作⽤通路:Anti-infection储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY⽣物活性HADAhydrochloride(HCC-Amino-D-alaninehydrochloride)⼀种蓝⾊荧光FDAA(λem~450nm)。FDAAs在肽聚糖(PG)合成位点有效地与多种细的PGs结合，允许在最⼩⼲扰下特异性和共价探测细的⽣长[1]。溶剂及浓度：DMSO：50mM体外研究1.Solutionpreparation[2]1.1PreparationofstocksolutionSolvent:DMSOConcentration:250mMisrecommended.Storage:Storeat-20°Cor-80°Cindarkafteraliquoting.Avoidrepeatedfreezingandthawing.1.2PreparationofworkingsolutionDiluteto250μMwithcellculturemedium(optimizedaccordingtotheexperiment).Note:Theworkingsolutionshouldbepreparedandusedimmediately.Keepitawayfromlight.2.S.aureusstrainsstaining2.1InoculatetheS.aureusstraininaculturedish.2.2AddHADAhydrochloride(250μM)tothecells.2.3Incubatecellsatroomtemperatureor37°Cfor30min(optimizedaccordingtotheexperiment).2.4Aspiratethedyeworkingsolution,wash2-3timeswithculturemedium,5minuteseachtime,andobserveusingafluorescencemicroscope.REFERENCES1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE[1].ErkinKuru,etal.SynthesisoffluorescentD-aminoacidsandtheiruseforprobingpeptidoglycansynthesisandbacterialgrowthinsitu.NatProtoc.2015Jan;10(1):33-52.[2].XuQ,etal.PhageproteinGp11blocksStaphylococcusaureuscelldivisionbyinhibitingpeptidoglycanbiosynthesis.mBio.2024Jun12;15(6):e0067924.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-820-3792;021