ICR-191-dihydrochloride-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEICR-191dihydrochlorideCat.No.:HY-W414334CASNo.:17070-45-0分⼦式:C₁₉H₂₃Cl₄N₃O分⼦量:451.22作⽤靶点:DNA/RNASynthesis;FluorescentDye作⽤通路:CellCycle/DNADamage;Others储存⽅式:Storeatroomtemperature,keepdryandcool*Insolvent:-80°C,6months;-20°C,1month(Storeatroom

temperature,keepdryandcool)BIOLOGICALACTIVITY⽣物活性ICR-191可增强Cisplatin(HY-17394)与DNA的结合，并使细胞对cisplatin敏感。ICR-191可显著增加磷酸化H2AX(γH2AX)的表达，尤其在DNA复制细胞中。ICR-191可⽤于⽩⾎病的研究[1][2]。体外研究ICR-191(16h)-inducedmutations(suchasGinsertion)createnewGGsitesinCHO-ICRcells,leadingtoanincreaseinthenumberofcisplatinbindingsites.TheIC50ofcisplatininCHO-ICRcells(IC50=15.96μM)isreducedtohalfthatofCHOwild-type(WT)cells(IC50=25.42μM)[1].ICR-191(8-12μM)fluorescenceisquenchedwithincreasingCHLconcentration[2].ICR-191(8-10.1μM)bindstoDNAviaintercalation,butitsaffinityisrelativelylow[2].ICR-191(1.2μM,1h)caninducehistoneH2AXphosphorylation(γH2AX)inHL-60cells,especiallyinS-phasecells[2].REFERENCES[1].ShaoC,etal.Pt-sequnveilsthegenomicbindingpatternofplatinum-baseddrugs.SciAdv.2025Oct31;11(44):eadx6809.[2].PietrzakM,etal.AttenuationofacridinemutagenICR-191--DNAinteractionsandDNAdamagebythemutageninterceptorchlorophyllin.BiophysChem.2008Jun;135(1-3):69-75.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-820-3792a