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4、 Nature, 2007, 448(7152): 457-460.,定向流动成膜,8,- Cote L J, Kim F, Huang J. Langmuir Blodgett assembly of graphite oxide single layersJ. Journal of the American Chemical Society, 2008, 131(3): 1043-1049.,L-B成膜,9,- Liu Z, Li Z, Xu Z, et al. Wet-spun continuous graphene filmsJ. Chemistry of Materials, 201

5、4, 26(23): 6786-6795.,湿纺,10,- Zangmeister C D, Ma X, Zachariah M R. Restructuring of graphene oxide sheets into monodisperse nanospheresJ. Chemistry of Materials, 2012, 24(13): 2554-2557.,11,- Han T H, Huang Y K, Tan A T L, et al. Steam etched porous graphene oxide network for chemical sensingJ. Jou

6、rnal of the American Chemical Society, 2011, 133(39): 15264-15267.,12,微观应用,13,- Kim J, Cote L J, Kim F, et al. Graphene oxide sheets at interfacesJ. Journal of the American Chemical Society, 2010, 132(23): 8180-8186.,Concentration Dependent Amphiphilicity,pH Dependent Amphiphilicity,Size Dependent A

7、mphiphilicity,14,- Tung V C, Huang J H, Tevis I, et al. Surfactant-free water-processable photoconductive all-carbon compositeJ. Journal of the American Chemical Society, 2011, 133(13): 4940-4947.,15,- Balapanuru J, Yang J X, Xiao S, et al. A Graphene OxideOrganic Dye Ionic Complex with DNASensing a

8、nd OpticalLimiting PropertiesJ. Angewandte Chemie, 2010, 122(37): 6699-6703.,16,- Lu C H, Yang H H, Zhu C L, et al. A graphene platform for sensing biomoleculesJ. Angewandte Chemie, 2009, 121(26): 4879-4881.,- Chang H, Tang L, Wang Y, et al. Graphene fluorescence resonance energy transfer aptasensor

9、 for the thrombin detectionJ. Analytical Chemistry, 2010, 82(6): 2341-2346.,17,1、只需要在一端标记荧光标签的,无需淬灭端。 2、荧光淬灭效果显著,背景噪音小。,- He Y, Wang Z G, Tang H W, et al. Low background signal platform for the detection of ATP: when a molecular aptamer beacon meets graphene oxideJ. Biosensors and Bioelectronics, 20

10、11, 29(1): 76-81.,MAB:aptamer molecular beacons,18,- Zhang M, Yin B C, Wang X F, et al. Interaction of peptides with graphene oxide and its application for real-time monitoring of protease activityJ. Chemical Communications, 2011, 47(8): 2399-2401.,Arg His Lys Trp Tyr Phe,静电,芳香氨基酸,19,- Shi X, Gu W,

11、Peng W, et al. Sensitive Pb2+ Probe Based on the Fluorescence Quenching by Graphene Oxide and Enhancement of the Leaching of Gold NanoparticlesJ. ACS applied materials & interfaces, 2014, 6(4): 2568-2575.,20,- Liu Z, Robinson J T, Sun X, et al. PEGylated nanographene oxide for delivery of water-inso

12、luble cancer drugsJ. Journal of the American Chemical Society, 2008, 130(33): 10876-10877.,21,22,- Zhang S, Yang K, Feng L, et al. In vitro and in vivo behaviors of dextran functionalized grapheneJ. Carbon, 2011, 49(12): 4040-4049.,- Bao H, Pan Y, Ping Y, et al. ChitosanFunctionalized Graphene Oxide as a Nanocarrier for Drug and Gene DeliveryJ. Small, 2011, 7(11): 1569-1578.,23,24,25,1 、处理贝壳,剪下外套膜和鳃; 2 、添加50 mM Tris-Hcl(pH 8.5)缓冲液于搅拌机中破碎;

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