会员注册 | 登录 | 微信快捷登录 支付宝快捷登录 QQ登录 微博登录 | 帮助中心 人人文库renrendoc.com美如初恋!
站内搜索 百度文库

热门搜索: 直缝焊接机 矿井提升机 循环球式转向器图纸 机器人手爪发展史 管道机器人dwg 动平衡试验台设计

   首页 人人文库网 > 资源分类 > DOC文档下载

[优秀毕业设计精品] 酱油中乙酰丙酸测定方法研究.doc

  • 资源星级:
  • 资源大小:787.36KB   全文页数:33页
  • 资源格式: DOC        下载权限:注册会员/VIP会员
您还没有登陆,请先登录。登陆后即可下载此文档。
  合作网站登录: 微信快捷登录 支付宝快捷登录   QQ登录   微博登录
友情提示
2:本站资源不支持迅雷下载,请使用浏览器直接下载(不支持QQ浏览器)
3:本站资源下载后的文档和图纸-无水印,预览文档经过压缩,下载后原文更清晰   

[优秀毕业设计精品] 酱油中乙酰丙酸测定方法研究.doc

毕业论文(设计)中文摘要I酱油中乙酰丙酸测定方法研究摘要由于生产工艺的不同,酱油可分为酿造酱油、水解酱油和配制酱油三种。这三种酱油的生产成本及营养价值差异很大,其中酿造酱油营养价值及生产成本最高,为有效防止其他种类的酱油冒充酿造酱油,保护消费者的利益,急需建立有效的可操作性的分析方法。酿造酱油中乙酰丙酸含量极微,而其他酱油中乙酰丙酸含量相对较高,所以酱油中乙酰丙酸的含量是区分酿造酱油和其他酱油的重要指标之一。本文采用高效液相色谱法研究了酱油中乙酰丙酸含量的测定方法,结果表明当色谱条件为LichrspherC18(150mm4.6mm,i.d.5μm),流动相甲醇水1585,流速1.0mL/min,检测波长266nm,进样体积5μL,采用了标准加入法定量,乙酰丙酸加入量与其峰面积在5.00~25.00mgmL1范围内有良好的线性关系,方法的重现性(RSD)为2.3。经用于实际样品分析,在酿造酱油中,其相关系数r为0.9995,平均加标回收率为99.1在配制酱油中,其相关系数r为0.9996,平均加标回收率为98.5。此法操作简单、快速,符合要求。关键词乙酰丙酸,高效液相色谱法,酱油毕业论文(设计)英文摘要IIStudyontheanalysismethodofLevulinicAcidinsoysauceAbstractSoysauceisconsistoftheblendedsoysauces,thehydrolyticsoysauceandfermentedsoysaucebecauseofdifferenttechniqueofproduction.Thesethreesoysaucesdiffergreatlyinproductioncostandnutritivevalue,andthefermentedsoysaucehasthehighestproductioncostandnutritivevalue.Inordertoavoidothersoysaucespersonatingthefermentedsoysauce,thereisanurgentneedtoestablisheffectiveandpracticablemethodtoprotectthebenefitofconsumers.Thecontentoflevulinicacidisrelativelylowerinthefermentedsoysauceandhigherinothersoysauces.Sothecontentoflevulinicacidinthesoysaucesisanimportantindextodistinguishthefermentedsoysaucesandothersoysauces.ThedeterminationoflevulinicacidinsoysaucehasbeenstudiedbymeansofHighPerformanceLiquidChromatographyHPLC.TheresultsindicatethatanalyticalconditionsincludecolumnC181504.6mm,5μm,mobilephasemethanolwater1585v/v,UVdetectionwavelength266nm,flowrate1.0mL/min,oventemperature25℃,injectionvolume5μL.Thestandardadditioncalibrationtechniqueisappliedinthepaper.Therearegoodlinearcorrelationsbetweentheconcentrationsandpeakareasofthecomponents,thelinearrangeisfrom5.00mgmL1to25.00mgmL1.Thereproducibilityofthecontentoflevulinicacidis2.3.Thismethodhasbeenappliedtotheanalysisofrealsamples.Theresultsshowthatthelinearcorrelationcoefficientis0.9995inthefermentedsoysauceand0.9996intheblendedsoysauce,andtheaveragerecoveryis99.1inthefermentedsoysauceand98.5intheblendedsoysauce.Theexperimentalresultsshowthatthemethodiseasyandquick.Andthismethodisuptothemustardtoo.KeywordsLevulinicacidHPLCSoysauce毕业论文(设计)目录III目录1绪论.......................................................................................................................11.1引言...................................................................................................................11.2乙酰丙酸的性质..................................................................................................11.3乙酰丙酸的提取分离方法....................................................................................11.3.1有机溶剂萃取法.......................................................................................21.3.2蛋白质沉淀法..........................................................................................21.3.3真空减压蒸馏法.......................................................................................21.3.4离子交换法..............................................................................................21.4乙酰丙酸的检测方法...........................................................................................31.4.1香草醛硫酸法........................................................................................31.4.2气相色谱法..............................................................................................31.4.3薄层扫描法..............................................................................................31.4.4离子色谱法..............................................................................................31.4.5高效液相色谱法.......................................................................................31.5乙酰丙酸的高效液相色谱法................................................................................42实验材料及原理..................................................................................................52.1实验材料............................................................................................................52.1.1实验药品..................................................................................................52.1.2实验仪器..................................................................................................52.2溶液的配制.........................................................................................................52.3实验原理............................................................................................................62.4实验方法............................................................................................................62.4.1样品前处理方法.......................................................................................62.4.2样品溶液的制备.......................................................................................62.4.3样品测定..................................................................................................62.4.4定性测定..................................................................................................62.4.5定量测定....................................................................................................73实验结果及讨论..................................................................................................83.1高效液相色谱法测定条件的选择........................................................................83.1.1液相色谱法检测器种类的选择....................................................................83.1.2实验测定波长的选择..................................................................................8毕业论文(设计)目录IV3.1.3色谱柱的选择.............................................................................................93.1.4流动相体系的选择......................................................................................93.1.5流动相配比的选择....................................................................................103.1.6流动相流速的选择...................................................................................123.1.7柱温的选择..............................................................................................133.1.8色谱分离条件的最终确定.........................................................................143.2标准曲线及线性范围的测定..............................................................................153.3精密度的测定...................................................................................................153.4稳定性试验.......................................................................................................163.5重复性试验.......................................................................................................163.6样品含量及回收率试验.....................................................................................173.6.1酿造酱油样品含量及回收率试验...............................................................173.6.2配制酱油样品含量及回收率试验...............................................................183.7新颖之处..........................................................................................................194结论.....................................................................................................................21参考文献...................................................................................................................22致谢...........................................................................................错误未定义书签。附表.....................................................................................................................24

注意事项

本文([优秀毕业设计精品] 酱油中乙酰丙酸测定方法研究.doc)为本站会员(zhuanyunshi)主动上传,人人文库网仅提供信息存储空间,仅对用户上传内容的表现方式做保护处理,对上载内容本身不做任何修改或编辑。 若此文所含内容侵犯了您的版权或隐私,请立即通知人人文库网([email protected]),我们立即给予删除!

温馨提示:如果因为网速或其他原因下载失败请重新下载,重复下载不扣分。

copyright@ 2015-2017 人人文库网网站版权所有
苏ICP备12009002号-5