常用酿酒酵母菌株基因型

Commonly used strains This page describes some of the most commonly used yeast lab strains. Much of the information is taken from F. Sherman (2002) Getting started with yeast, Methods Enzymol. 350, 3-41. Other useful papers for strain background information include: Mortimer and Johnston (1986) Genetics 113:35-43 - thoroughly describes the genealogy of strain S288C van Dijken et al. (2000) Enzyme Microb Technol 26:706-714 - compares various characteristics of commonly used lab strains Winzeler et al. (2003) Genetics 163:79-89 - uses SFP (single-feature polymorphisms) analysis to study genetic identity between common lab strains S288C Genotype: MAT SUC2 gal2 mal mel flo1 flo8-1 hap1 ho bio1 bio6 Notes: Strain used in the systematic sequencing project, the sequence stored in SGD. S288C does not form pseudohyphae. In addition, since it has a mutated copy of HAP1, it is not a good strain for mitochondrial studies. It has an allelic variant of MIP1 which increases petite frequency. S288C strains are gal2- and they do not use galactose anaerobically. The S288C genome was recently resequenced at the Sanger Institute. References: Mortimer and Johnston (1986) Genetics 113:35-43. Sources: ATCC:204508 BY4743 Genotype: MATa/ his31/his31 leu20/leu20 LYS2/lys20 met150/MET15 ura30/ura30 Notes: Strain used in the systematic deletion project, generated from a cross between BY4741 and BY4742, which are derived from S288C. As S288c, these strains have an allelic variant of MIP1 which increases petite frequency. See Brachmann et al. reference for details. References: Brachmann et al. (1998) Yeast 14:115-32. Sources: Biosystems:YSC1050 FY4 Genotype: MATa Notes: Derived from S288C. References: Winston et al. (1995) Yeast 11:53-55. Brachmann et al. (1998) Yeast 14:115-32. FY1679 Genotype: MATa/ ura3-52/ura3-52 trp163/TRP1 leu21/LEU2 his3200/HIS3 GAL2/GAL Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD. References: Winston et al. (1995) Yeast 11:53-55. Sources: EUROSCARF:10000D AB972 Genotype: MAT X2180-1B trp10 rho 0 Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD. AB972 is an ethidium bromide-induced rho- derivative of the strain X2180-1B-trp1. References: Olson MV et al. (1986) Proc. Natl. Acad. Sci. USA 83:7826-7830. Sources: ATCC:204511 A364A Genotype: MATa ade1 ade2 ura1 his7 lys2 tyr1 gal1 SUC mal cup BIO Notes: Used in the systematic sequencing project, the sequence stored in SGD. References: Hartwell (1967) J. Bacteriol. 93:1662-1670. Sources: ATCC:208526 XJ24-24a Genotype: MATa ho HMa HM ade6 arg4-17 trp1-1 tyr7-1 MAL2 Notes: Derived from, but not isogenic to, S288C References: Strathern et al. (1979) Cell 18:309-319 DC5 Genotype: MATa leu2-3,112 his3-11,15 can1-11 Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD. References: Broach et al. (1979) Gene 8:121-133 X2180-1A Genotype: MATa SUC2 mal mel gal2 CUP1 Notes:S288c spontaneously diploidized to give rise to X2180. The haploid segregants X2180-1a and X2180-1b were obtained from sporulated X2180 References: Mortimer and Johnston Sources: ATCC:204504 YNN216 Genotype: MATa/ ura3-52/ura3-52 lys2-801amber/lys2-801amber ade2-101ochre/ade2-101ochre Notes: Congenic to S288C (see Sikorski and Hieter). Used to derive YSS and CY strains (see Sobel and Wolin). References: Sikorski RS and Hieter P (1989) Genetics 122:19-27. Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862. YPH499 Genotype: MATa ura3-52 lys2-801_amber ade2-101_ochre trp1-63 his3-200 leu2-1 Notes: Contains nonrevertible (deletion) auxotrophic mutations that can be used for selection of vectors. Note thattrp1-63, unlike trp1-1, does not delete adjacent GAL3 UAS sequence and retains homology to TRP1 selectable marker.gal2-, does not use galactose anaerobically. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C. References: Sikorski RS and Hieter P (1989) Genetics 122:19-27. Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862. Johnston M and Davis RW (1984) Mol Cell Biol 4(8):1440-8. Sources: ATCC:204679 YPH500 Genotype: MAT ura3-52 lys2-801_amber ade2-101_ochre trp1-63 his3-200 leu2-1 Notes:MAT strain isogenic to YPH499 except at mating type locus. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C. References: Sikorski RS and Hieter P (1989) Genetics 122:19-27. Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862. Johnston M and Davis RW (1984) Mol Cell Biol 4(8):1440-8. Sources: ATCC:204680 YPH501 Genotype: MATa/MAT ura3-52/ura3-52 lys2-801_amber/lys2-801_amber ade2-101_ochre/ade2-101_ochre trp1- 63/trp1-63 his3-200/his3-200 leu2-1/leu2-1 Notes: a/ diploid isogenic to YPH499 and YPH500. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C. References: Sikorski RS and Hieter P (1989) Genetics 122:19-27. Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862. Johnston M and Davis RW (1984) Mol Cell Biol 4(8):1440-8. Sources: ATCC:204681 Sigma 1278B Notes: Used in pseudohyphal growth studies. Detailed notes about the sigma strains have been kindly provided by Cora Styles. Granek and Magwene, PLoS Genet. 2010 Jan 22;6(1):e1000823, established that certain lineages of the Sigma1278B background contain a nonsense mutation in RIM15, a G-to-T transversion at position 1216 that converts a Gly codon to an opal stop codon. This rim15 mutation interacts epistatically with mutations in certain other genes to affect colony morphology. Annotation of the Sigma1278b genome and information about the systematic deletion collection can be found here. SK1 Genotype: MATa/ HO gal2 cupS can1R BIO Notes: Commonly used for studying sporulation or meiosis. Canavanine-resistant derivative. The SK1 genome was sequenced at the Sanger Institute. References: Kane SM and Roth J. (1974) Bacteriol. 118: 8-14 Sources: ATCC:204722 CEN.PK (aka CEN.PK2) Genotype: MATa/ ura3-52/ura3-52 trp1-289/trp1-289 leu2-3_112/leu2-3_112 his3 1/his3 1 MAL2-8C/MAL2-8C SUC2/SUC2 Notes: CEN.PK possesses a mutation in CYR1 (A5627T corresponding to a K1876M substitution near the end of the catalytic domain in adenylate cyclase which eliminates glucose- and acidification-induced cAMP signalling and delays glucose-induced loss of stress resistance (Vanhalewyn et al., 1999; Dumortier et al., 2000). References: van Dijken et al. (2000) Enzyme Microb Technol 26:706-714 Sources: EUROSCARF:30000D W303 Genotype: MATa/MAT leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15 phi+ Notes: W303 also contains a bud4 mutation that causes haploids to bud with a mixture of axial and bipolar budding patterns. In addition, the original W303 strain contains the rad5-535 allele. As S288c, W303 has an allelic variant ofMIP1 which increases petite frequency. The W303 genome was sequenced at the Sanger Institute. References: W303 constructed by Rodney Rothstein (see detailed notes from RR and Stephan Bartsch). bud4 info: Voth et al. (2005) Eukaryotic Cell, 4:1018-28. rad5-535 info: Fan et al. (1996) Genetics 142:749 Sources: Biosystems:YSC1058 W303-1A Genotype: MATa leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15 Notes: W303-1A possesses a ybp1-1 mutation (I7L, F328V, K343E, N571D) which abolishes Ybp1p function, increasing sensitivity to oxidative stress. References: W303 constructed by Rodney Rothstein (see detailed notes from RR and Stephan Bartsch). ybp1-1 info: Veal et al. (2003) J. Biol. Chem. 278:30896-904. Sources: Biosystems:YSC1058 W303-1B Genotype: MAT leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15 References: W303 constructed by Rodney Rothstein (see detailed notes from RR and Stephan Bartsch). Sources: Biosystems:YSC1058 W303-K6001 Genotype: MATa; ade2-1, trp1-1, can1-100, leu2-3,112, his3-11,15, GAL, psi+, ho:HO:CDC6 (at HO), cdc6:hisG, ura3:URA3 GAL-ubiR-CDC6 (at URA3) References: K6001 was developed by Bobola et al in Kim Nasmyths lab (PMID: 8625408), and has become a common model in yeast aging research (PMID: 15489200). Its genome has been sequenced by Timmermann et al (PMID: 20729566) D273-10B Genotype: MAT mal Notes: Normal cytochrome content and respiration; low frequency of rho-. This strain and its auxotrophic derivatives were used in numerious laboratories for mitochondrial and related studies and for mutant screens. Good respirer thats relatively resistant to glucose repression. References: Sherman, F. (1963) Genetics 48:375-385. Sources: ATCC:24657 FL100 Genotype: MATa References: Lacroute, F. (1968) J. Bacteriol. 95:824-832. Sources: ATCC: 28383 SEY6210/SEY6211 Genotype: MATa/MAT leu2-3,112/leu2-3,112 ura3-52/ura3-52 his3-200/his3-200 trp1-901/trp1-901 ade2/ADE2 suc2-9/suc2-9 GAL/GAL LYS2/lys2-801 Notes: SEY6210/SEY6211, also known as SEY6210.5, was constructed by Scott Emr and has been used in studies of autophagy, protein sorting etc. It is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sherman, Randy Schekman). It has several selectable markers, good growth properties and good sporulation. References: Robinson et al. (1988) Mol Cell Biol 8(11):4936-48 Sources: ATCC:201392 SEY6210 Genotype: MAT leu2-3,112 ura3-52 his3-200 trp1-901 suc2-9 lys2-801; GAL Notes: SEY6210 is a MATalpha haploid constructed by Scott Emr and has been used in studies of autophagy, protein sorting etc. It is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sherman, Randy Schekman). It has several selectable markers and good growth properties. References: Robinson et al. (1988) Mol Cell Biol 8(11):4936-48 Sources: ATCC:96099 SEY6211 Genotype: MATa leu2-3,112 ura3-52 his3-200 trp1-901 ade2-101 suc2-9; GAL Notes: SEY6211 is a MATa haploid constructed by Scott Emr and has been used in studies of autophagy, protein sorting etc. It is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sherman, Randy Schekman). It has several selectable markers and good growth properties. References: Robinson et al. (1988) Mol Cell Biol 8(11):4936-48 Sources: ATCC:96100 JK9-3d There are a, alpha and a/alpha diploids of JK9-3d with the following genotypes: Genotypes: JK9-3da MATa leu2-3,112 ura3-52 rme1 trp1 his4 JK9-3d has the same genotype as JK9-3da with the exception of the MAT locus JK9-3da/ is homozygous for all markers except mating type Notes: JK9-3d was constructed by Jeanette Kunz while in Mike Halls lab. She made the original strain while Joe Heitman isolated isogenic strains of opposite mating type and derived the a/alpha isogenic diploid by mating type switching. It has in its background S288c, a strain from the Oshima lab, and a strain from the Herskowitz lab. It was chosen because of its robust growth and sporulation, as well as good growth on galactose (GAL+) (so that genes under control of the galactose promoter could be induced). It may also have a SUP mutation that allows translation through premature STOP codons and therefore produces functional alleles with many point mutations. References: Heitman et al. (1991a) Science 253(5022):905-9 and Heitman et al. (1991b) Proc Natl Acad Sc