中科院2005年攻读硕士学位研究生入学试题《生物化学及分子生物学》.docVIP

2005 20201.530.1234GRas56KmpH78ubiquitination-9NADHQ10110.154MpH12ATPATPCa2+/2H+13INAD+ IINADP+ACoAFMNFADAMP14telomeraseRNARNA; 15TmDNADNA9016DNA17ISDNA1819RNAIICRNAII20DNA30301.545.1 A.B.C.D.2 ABCD3.cAMP ACBA CGDB4 ABCD5AG BCD,/6ABC7AHisSerCys BHisLysArg CAspGluPhe DAsnGlnTrp8 ABC9EGFTGFAB/C/D/10ATPA BCD11AKm BKmC D12pHA His- BCys-CLys- DArg-13W/V) A0.1%B0.5% C2.5% D10%14CpI A,6; B,6-7 C7-8 D815,A BNADH CQ-cDc16A B C17EPO ABCD18-3- ABCD19SangerDNADNADNAA B53 C3520DNACpGCpG islandAC3m3CpG; B,C 5m5CpGCG3 Cpm3G DG7Cpm7G21RNA A,rRNA; B,mRNA; C,tRNA; D,snRNA22,DNAADNAIKornborg BDNAII CDNAIII DDNAIV23BDNAZDNA A B C24-5-ATP5-1-PRPPABCDATP25DNAAzinc finger Bleucine zipperC-helix-loop-helix D-helix-turn-helix26retrovirusDNAAtRNA BmRNA CrRNA D5SrRNA27RNAIIIAtRNA5SrRNArRNA BsnRNACrRNAD28DNARecADNAADNABDNACDNADDNADNA29GH A; B, C D30ABCDDNA55251SH2SH32S1 S2KcatVmaxKm S1S2S1S2 34RNAnon-coding RNA1RNAsmall nucleolar RNA,snoRNA);(2)RNAsmall interfering RNA,siRNA);(3)RNAmicroRNAmiRNA5mRNAalternative splicing) 510501A2 2345GAL4 yeast two hybrid system20051- 2- 3+ G4+5- 6- KmpH7+ 8+ 9- NADH10- 11+ K+K+H+pH12- 13- FMN14+15- DNA50 16-17- 18- 19- 20+1D2A 3BcAMPcAMPAcAMPcAMP4CNOL-5C6B7A Ser His Cys8C Kcat9D10B ATP11C12A13D 20%-30%14D cpI10.2515D16B 17B18A D-3-NAD+D-3+13-GAPDH4333GAPDH145ku19B20B21C22C DNADNADNADNADNA23B24C PRPP5-ATP25D26A27A RNA28D29C30B 1SH2SH3Pro2ES1/ES2=Km2/Km1 V1/V2=Kcat1ES1/Kcat2ES2 3 -CoA4rRNARNAdsRNA21-23RNA.siRNARNA425mRN