Mouse Embryonic Stem Cells 胚胎干细胞.ppt

Mouse Embryonic Stem (ES) Cell Basal Medium 干细胞培养专用基础培养基 （小鼠）,Cat. No. 01-171-1B Cat. No. 01-171-1A,BIOLOGICAL INDUSTRIES,Mouse embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of the blastocyst. 小鼠干细胞是从内细胞聚集的胚泡 中提取的 Undifferentiated ES cells can be maintained in vitro for extended periods without loss of their capacity to differentiate to all cell lineages when re-implanted back into a blastocyst. 未分化的干细胞在体外培养一段时间后，当重新种植回胚泡时，能够不丢失他们的分化成各种子细胞系的能力 ES cells may differentiate in vitro to a variety of cell types including neuronal, muscle, endothelial and hematopoietic progenitors. 干细胞能在体外分化成多种细胞类型包括神经原细胞，肌肉细胞，内皮细胞和造血源细胞 General culture conditions are well established and usually require ES cells to be grown on an inactive feeder cell layer or on gelatin-coated plates with Leukemia Inhibitory Factor (LIF) in the culture medium. 一般的培养条件建立起来后，通常需要干细胞在不活跃的环境生长，或 在凝胶包被的带有杀伤细胞抑制因子的培养基中生长,Mouse ES Basal Medium has been optimized to grow and maintain undifferentiated mouse embryonic stem cells. 小鼠干细胞基础培养基优化后可用作维护和生长未分化的小鼠干细胞 The medium may be used with the addition of Foetal Bovine Serum (FBS) or with any serum replacement designed for mouse ES cells. 培养基可以和牛血清一起使用或可以和任何为小鼠干细胞培养专门设计的添加剂一起使用 The medium contains stable glutamine dipeptide, and is therefore stable for at least 18 months from production, when stored at +2-8C. 培养基含有一定量的谷氨酰胺二肽，因此可以在+2-8C时保持至少个月的稳定性,Direct comparison to DMEM:F12 or to a commonly used commercial medium demonstrate superior results, when used with either Foetal Bovine Serum (FBS) or Serum Replacement (SR) see figures 1 and 2. 当和牛血清或血清替代品一起使用时,此培养基与DMEM:F12比较或常用的商业培养基相比，有很好的效果,Figure 1: Growth rate of mouse ESC (ES-D3) using the Mouse ES Basal Medium vs. KO DMEM (Invitrogen) with KO Serum Replacement (SR; Invitrogen). 使用小鼠基础培养基和KO DMEM (Invitrogen)及血清替代品(SR; Invitrogen)时，小鼠细胞ESC (ES-D3)生长率的比较 mESC were cultivated in a 6w/p and observed over a period of 5 days. mESC被培养每天观察和纪录个临界点的值 Proliferation rate was obtained by using trypan blue, a dye exclusion procedure for viable cell counts. A hemocytometer was used to determine total cell count and viable cell numbers. 通过使用台酚兰染色进行增殖率的比较，通过血球记数来决定细胞总数和存活细胞数,Figure 2: Growth rate of mouse ESC (ES-D3) using the mouse ES Basal Medium vs. KO DMEM (Invitrogen). 使用小鼠基础培养基和KO DMEM (Invitrogen)，小鼠细胞ESC (ES-D3)生长率的比较 mESC were cultivated in a 96w/p and observed over a period of 5 days. mESC在天内共小时的培养比较 Proliferation rate was measured using a colorimetric method (XTT based Cell Proliferation Kit , Cat. No. 20-300-1000). 增殖率的检测通过比色法（细胞增殖XTT试剂盒， Cat. No. 20-300-1000） Absorbance was read afte