青霉素的文献调研解析.ppt

关于青霉素检测的相关文献,青霉素（Penicillin，或音译盘尼西林）是抗菌素的一种，是指分子中含有青霉烷、能破坏细菌的细胞壁并在细菌细胞的繁殖期起杀菌作用的一类抗生素，是由青霉菌中提炼出的抗生素。青霉素属于内酰胺类抗生素（-lactams）,内酰胺类抗生素包括青霉素、头孢菌素、碳青霉烯类、单环类、头霉素类等。青霉素是很常用的抗菌药品。,鉴于青霉素的潜在危害，为有效规范青霉素类药物在医疗和畜牧业中的应用，各国或地区对青霉素的最大残留限量( MRL) 作了明确规定 。美国FDA规定，青霉素类抗生素在各类农产品中的MRL不超过5 gkg-1，欧盟对牛奶中青霉素的MRL规定: 青霉素G 40 gL-1，苯唑青霉素、邻氯青霉素、双氯青霉素均为30 gL-1，我国农业部规定，氨苄青霉素在可食用动物组织中的MRL均为50 gkg-1。,青霉素,氨苄青霉素,青霉素残留检测方法,目前，青霉素残留检测方法可归纳为以下几类: 微生物法； 仪器分析法( 包括高效液相色谱法、高效液相色谱-串联质谱法和气相色谱法等)； 免疫分析法( 如酶联免疫分析法和免疫传感器等)； 基于受体技术的分析法（化学发光分析法、适配子法、基于受体技术的分析法）。,-Song K M, Jeong E, Jeon W, et al. Aptasensor for ampicillin using gold nanoparticle based dual fluorescencecolorimetric methodsJ. Analytical and bioanalytical chemistry, 2012, 402(6): 2153-2161.,图1 ampicillin的三种适配体,使用核酸适配体在溶液中检测氨苄青霉素的文章：,图2 使用AMP17 ssDNA aptamer的荧光-比色法检测ampicillin,The lower limits of detection for this dual method were a 2 ng/mL by fluorescence and a 10 ng/mL by colorimetry for ampicillin in the milk as well as in distilled water.,-Wang X, Dong S, Gai P, et al. Highly sensitive homogeneous electrochemical aptasensor for antibiotic residues detection based on dual recycling amplification strategyJ. Biosensors and Bioelectronics, 2016, 82: 49-54.,Fig.1 Principle of target-induced and T7 exonuclease-aided recycling amplification homogeneous electrochemical strategy for highly sensitive detection of AMP. (For interpretation of the references to color in this figure, the reader is referred to the web version of this article.) AMP: ampicillin,Low detection limit: 4.0 pM,本文研究的方 法是将玻碳电极进行氨基化修饰 , 通过碳二亚胺盐酸盐 ( EDC) 、 N - 羟基丁二酰亚胺 (NHS) 活化作用将适配子共价结合在电极表面 , 通过循环伏安法测得的电化学信号检测标本中青霉素类抗生素浓度 。该适配子电化学生物传感器分子识别能力强 、无放射性 标记 、检测速率快 , 青霉素类的最佳检测范围是 2. 81 281 nmol/L , 最低检测限为 2. 81 nmol/L , 检测时间为 5 min.,-范婷, 张娟琨, 李敏, 等. 适配子生物传感器对青霉素的快速检测J. 生物加工过程, 2011, 9(3): 41-46.,In the present analyze, we have beneficially displayed the fabrication of a silver nanoparticles based biosensor with good reproducibility and selectivity for fast monitoring of penicillin with immobilization of penicillinase enzyme by simple physical adsorption method. Main equation for this research was: Penicillin + H2O = penicilloate+ H+,-Sistani P, Sofimaryo L, Masoudi Z R, et al. A Penicillin Biosensor by Using Silver NanoparticlesJ. Int. J. Electrochem. Sci, 2014, 9: 6201-6212.,-Broto M, Matas S, Babington R, et al. Immunochemical detection of penicillins by using biohybrid magnetic particlesJ. Food Control, 2015, 51: 381-389.,The aim of this work is focused on the production of polyclonal antibodies using a new immunizing hapten and the necessary immunoreagents to establish an immunochemical assay for penicillins detection in milk samples. limits of detection (0.1mgL-1),利用抗原抗体特异性反应的文章：,-Byzova N A, Zvereva E A, Zherdev A V, et al. Immunochromatographic technique for express determination of ampicillin in milk and dairy productsJ. Applied biochemistry and microbiology, 2011, 47(6): 627-634.,An immunochromatographic method for determination of -lactam antibiotic ampicillin has been developed. The method is based on the competitive interaction between antibiotic molecules contained in the sample and protein conjugate of penicillin immobilized on a membrane for binding with specific antibodies labeled with colloidal gold, which occurs during movement of the sample to be tested and reagents along the membrane.,The coloration in the test zone was observed in the dilute sample containing ampicillin at concen- trations lower than 10 ng/mL (in the starting sample, at a concen-tration lower than 13.3 ng/mL).,-Li H, Xu B, Wang D, et al. Immunosensor for trace penicillin G detection in milk based on supported bilayer lipid membrane