人类疱疹病毒-1精美生物医学.ppt

人 类 疱 疹 病 毒,Human herpes viruses,Contains several of the most important human pathogens A spectrum of diseases Life long persistent infection Periodic reactivation,Main properties,Virion: spherical, 150-200nm core + capsid + envelope dsDNA, encodes at least 100 proteins 35 proteins structure of virus Replication: In the nucleus Bud from nuclear membrane Early replication and late replication,CPE: apart from EBV, others can cause CPE in cell culture Form of infection Replication infection : primary, reactivated Latent infection Congenital infection (先天性感染) Cell transformation,Herpes simplex virus (HSV),Properties Two serotypes : HSV1 and HSV2 HSV genome: encodes at least 70 proteins some associated with viral replication some envelope proteins associated with immune reaction viral penetration,Herpes simplex virus （ HSV ）,Pathogenicity,Source of infection patients and carriers Routes of transmission HSV1： contact HSV2： sexually / maternal genital infection Pathology :cytolytic infection,Primary infection most are inapparent , but result in antibody production and latent infection,HSV1 Gingivitis (齿龈炎): most frequently Keratoconjunctivitis (角膜结膜炎) Skin infection Encephalitis Latent infection : in the trigeminal ganglia (三叉神经节),HSV2 Genital herpes: common seen Skin herps : as HSV1 Neonatal herpes : during birth, is a severe disease skin, eye, month lesion encephalitis multiple organ disseminated (多器官感染) Latent infection：sacral ganglia (骶神经节),Latent and recurrence infection HSV1,HSV2 neuron virus reactivate , replication axon (神经纤维) peripheral sites, skin or mucous membrane HSV1：口唇部疱疹 HSV2：genital herpes,Varicella-zoster virus （VZV） 水 痘 带 状 疱 疹 病 毒,similar as HSV,VZV naked,VZV enveloped,Pathogenicity Source of infection：patients Routes of infection: respiratory tract Disease： 水痘(Varicella/chickenpox)： 儿童：斑丘疹，水疱，偶见并发症 孕妇：症状较重，可致胎儿畸形，流产，死胎 免疫低下：重症水痘 Latent infection dorsal root ganglia (脊髓后根神经节),Zoster (带状疱疹) Seen in adults Severe pain and vesicle, often in chest,Cytomegalovirus (CMV) 巨 细 胞 病 毒,The largest genetic content of HHV Very species specific and cell specific (种属和细胞特异性) Characteristic CPE: multinucleated cells and intranuclear inclusion Is spread cell to cell,Pathogenicity,Source of infection: patients / inapparent patients Routes of infection Close contact Mother to child,Diseases,Normal host Most are subclinical Mononucleosis like syndrome (单核细胞增多症) a mild illness Latent infection Immunosuppressed host Much more severe Disseminated pneumonia In AIDS: 胃肠炎，视网膜炎,Congenital and perinated infection 先天和围产期感染 May be severe Fetal and new born Cytomegalic inclusion disease (巨细胞包涵体病) Death of fetus C.N.S defect : 耳聋，智力发育迟缓 An important public problem,Epstein-Barr virus EBV,Viral antigens Latent phase Ag EBNA1: latent infection EBNA2: transformation of cells LMP: latent infection transform cells Replication Ag Early Ag: viral replication Late Ag: structure proteins,Pathogenecity,Speared by contact : Primary infection: more unapparent Latent infection Cell transformation,Diseases Infectious mononucleosis Transmitted by infected saliva More seen in adolescents and young adults Fever, sore throat, WBC incraesed Distinguish with leukemia heterophil agglutination (异嗜凝集试验)：heterophil Ab can agglutinate sheep RBC Bu